Agar Well Diffusion Assay | Determination Of MIC By Agar Diffusion Method |

[@neharaturi3853]

Well explained sir thank u😊

[@rehobothica1535]

Nice Job; I enjoyed this

[@de.de.3s]

IZ What does it mean?

[@babusheikh6652]

Dear sir, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?

[@tamerwali4598]

Does this give the same mic result as tube dilution methods?

[@RndmClp]

Is this process also good for antifungal test? Or there is another process for antifungal test?

[@hksm87]

Same can be used. But you have to make sure the amount of conidia s required in 1ml by hemocytometer.

[@WINmhadekCH]

Hello can you tell me what do control paper disk use to?

[@hksm87]

Positive and negative controls are required for proper analysis.

[@nivedaponmani8945]

After loading the samples in the well and while keeping it in the incubator the petridish should be kept inverted or not?

[@BMHlearning]

Petri dishes need to be incubated upside-down to lessen contamination risks from airborne particles landing on them and to prevent the accumulation of water condensation that could disturb or compromise a culture

[@shimeapagurayan1851]

Hello, we have a problem with the bacterial suspension drying out in the well

[@hksm87]

It's okay. No worries.

[@asmaabubaker7456]

Thank you so much for this

[@BMHlearning]

Any time!

[@trickibaba8386]

Hii sir I really needs your help. Can we use this method for checking the antifungal activity of any substance. Please reply

[@BMHlearning]

I think u can use it... U are just replacing the antibacterial with antifungal. I suggest u to go through scientific literature for more details.