Cell Fractionation

[@hak8755]

man u make me cry . i wished u had make thisssss before . u know how much time i have spent to learn this staff . u are very teacher and u explain staff veryyyyyyy goood . thanks

[@AKLECTURES]

h ak lol thanks!! :)

[@fk2328]

better teaching than my biology teacher at school

[@eddiele3939]

5:12 clear view of the board

[@safeenagul2768]

Thanku sir Bcoz of u I understand this topic otherwise I miss it thanks alot keep it up God bless you sir

[@stanislavyordanov6909]

You are awesome, it's the most helpful channel when it comes to proteins, thanks, thanks, thanks!!!!

[@AKLECTURES]

glad to hear that!

[@poutineprin2210]

Thank you so much! This was very clear and helpful.

[@jamshidahmad8971]

xcellent sir, realy enjoyed ur lecture and learnt alot

[@casualcasual1234]

I would like to ask why grinding cells only break plasma membrane, how can we control such that the membranes of organelles are not broken during this process?

[@fk2328]

@Tony Lee before cell fractionation takes place, the organelles are buffered, isotonic, and placed in a cold solution. Buffering - so that the pH doesn’t change and therefore the enzyme structure/function doesn’t alter (change). Isotonic - has the same water potential inside + outside to PREVENT ORGANELLES from shrivelling/bursting. Placed in a cold solution - to reduce ENZYME ACTIVITY so that the enzymes can’t break down the organelles. Hope that helps.

[@casualcasual1234]

@@fk2328 Thanks a lot for the explanations for each step, now I understand clearly :) 1 quick question: can we also add some protease inhibitors (cocktails) together with the cold solution to further minimize digestion?

[@norrisjanoudi6771]

Thank you! It is a very helpful lecture! but i have hard time seeing which one would be the next session you mentioned in the video.

[@AKLECTURES]

+Nawras Janoudi Check out my website for a ordered playlist

[@norrisjanoudi6771]

+AK LECTURES (Andrey K) I think i have found a gem! your website is awesome and the videos are extremely helpful! I really appreciate your hard work.

[@samantahir1442]

You made it so easy thank you so much

[@brnygtg281]

Thanks for whole your videos about biochemistry.

[@kk-sz2lv]

How long are the tubes centrifuged for? (at 1000gs and 10000 gs)

[@miramaltouhamy2981]

thanks a lot. could you talk about ultracentrifugation ?

[@samflores3332]

Can you please describe and explain how cell fractionation and ultracentrifugation can be used to isolate mitochondria from a suspension of animal cell @AK lectures

[@protoxpire0]

if we want to separate the proteins, why can't we centrifuge at 100,000g's at once and get the supernatant?

[@adeshkumarmahraj5014]

Love from Pakistan 💕💯

[@arunkumars6257]

Thank you so much!

[@pabzbestpredicts4040]

Thank you for your wonderful presentation, can you please do ‘flow cytometry

[@kimpham3695]

I love you!

[@ridewya]

your the best

[@AKLECTURES]

thanks!

[@chiomaokeke1119]

ily

[@ogagadortie9487]

I can't a thing of what you're saying. you're too fast sir