Affinity Chromatography

[@alejandrocanas6744]

The homie AK getting me through all of undergrad, and I already know he'll be with me all throughout med school haha. Thank you bruda!

[@AKLECTURES]

Definitely will! Creating a ton of new content geared for medical school :)

[@alejandrocanas6744]

Awesome!!!! :D

[@kennethkyle1766]

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[@kashcole5038]

@Kenneth Kyle instablaster :)

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@@kennethkyle1766 If you can't reset it through your email, or you don't have access to your email, I suggest contacting insta support

[@thekillfreaks]

I love you man, my prof can't teach, first exam tomorrow and im dying

[@jchae9820]

no such words could EVER show how thankful i am for you... my medical studies are in french and i don't get a word of what our prof say... but here you are explaining better than any prof could ever do!!! such a HERO

[@kylieharris3569]

Amazing. Getting me thru undergrad and now job interviews in the biotech industry. Thank you!

[@nurafifahmursyida3304]

totally help me to understand more about the differences between ion chromatography and affinity chromatography. thank you very much!

[@asniadach99]

you are the best! I cannot tell you how much your lectures have helped me through my biochemistry degree

[@joycetapia1932]

It is INSANE how well you lecture WOW THANK YOU SO MUCH

[@kshitijagadekar4916]

You are simply excellent!!!!!!! the way you cover the points and also the use of diagrams is really helpful!!! thanks alot!

[@marieclaire5580]

I‘m a German student and I can’t believe how easy it was to understand you. Thank you, you helped me a lot

[@RockResilient]

I wonder what do you do now

[@mary-annbuchanan9091]

Last exam of the year tomorrow and this helps heaps!

[@carolinegreen4771]

#Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures. The separation is based on differential partitioning between the mobile and stationary phases. Commonly used chromatography techniques include: gel filtration, ion exchange chromatography, hydrophobic interaction chromatography and affinity chromatography. Creative BioMart contains all different types of chromatography at all scales of matrix include: cross-linked agarose, cross-linked cellulose, dextran, methacrylic and polystyrene.

[@MelissaMaiga]

Wow. This seems so simple! I feel like I can explain it to anyone!

[@Ahmed-tu3ms]

The greatest in biochemistry THANKS

[@josephineelvina5281]

Tomorrow i have final exam on enzymology,then i am here to understand this course and it is very helpful🥺❤️

[@nimishadutta]

All your videos make all the topics so easy to understand... Thanks! :)

[@milicialina3193]

Why will the washout glucose solution outcompete the glucose on the beads?

[@hannacastano7236]

Your lectures are so clear ! thanks

[@AjayiChristianah-i9m]

I love how hou you explain the topics

[@josejr7278]

If you watch the entire video at 1.75x speed this dude is aggressively teaching you

[@stijnvanmegen1032]

GREAT! you're a really good teacher, keep it up ;D

[@AKLECTURES]

Stijn Vanmegen Thanks, appreciate that :)

[@tranthanhhoang2726]

Pardon, it is great for me to have the image after you ( I mean your lecture). Thanks, Sir.

[@dannyl3626]

thank you so much for ALL THESE WONDERFUL VIDEOS, they help me a lot to study biochemistry!

[@shahriarmeraj6746]

This man is like oxygen for a student like me studying in a subject like biochemistry and molecular biology..... 😰😰😰

[@kay22lovely]

this is a great video and I found it to be a helpful review for my studies. However as a friendly correction you stated that this technique is only useful "if you know what specific protein that molecule binds to" However, I believe you can use Affinity chromotography for undetermined binding. If you use s tag such as FLAG that is is very hydrophilic it can be used to bind / tag the protein to the column even if that have no known binding partner. Then you can look at similar family of proteins with known/potential binding partners and run those partners through the column to see what your proteins of interest binds to. This technique has been used in many publications such as " Optimal Translational Termination Requires C4 Lysyl Hydroxylation of Erf1" Feng Et AL. Again just a friendly correction. Thank you .

[@harisbinfida6624]

A great teacher ...really impressed by your way of teaching . Got to know about your lectures by random searching.Your dedication is reflecting in ur lectures ...

[@nbtquoc]

dam, you are the best teacher in the World

[@ramaos8542]

You are a life saver!

[@markishamcclenton3399]

Awesome informational video. Thanks!!

[@debahutiborthakur7820]

thank you so much.... you are a perfect teacher.

[@aseelbiology4468]

Thank you for your hard work 👍

[@davidd2008]

Thanks for teaching easley!🌟

[@antheliax1625]

great video !! really comprehnesive ! thanks a lot for making it so easy !

[@leo-r7]

you are a biochemistry God!

[@crystal.a62]

Thank you very much for this useful videos😃

[@chenksR]

Thank-you for doing this! People like you are awesome.

[@youknowitis]

Very nice Explanation 👍 Very helpful 👌👌

[@MogulGroup1985]

I'm your biggest fan man! Thank you

[@BiopureNewsNetworkWashington]

use displace instead of outcompete for reversible reactions.

[@mohammedodeh7348]

Thank you....

[@meadowsanddawn7464]

Thank you very much, your lectures are very helpful :D

[@ashfaqalam9785]

Great lecture Ever... thanku Sir

[@eetezaznafie964]

Really you are amazing lecture i used to follow your lectures,but in this one i have a notes which is whats make free sugar solution pull glucose oxidase from its attracted state ?

[@juicyalavida]

Thank you thank you so much! You're a lifesaver!!

[@PandaTheGreen]

your videos have helped me a lot, thanks!

[@asoomf3364]

thank you keep going that was very very helpfull

[@raghadhammami5364]

Thank you ♥️♥️ you’re the best

[@lyeerg9896]

How do we separate the protein from the glucose solution once collected?

[@emanfathy1016]

that was awesome , Thanks

[@davegilb3015]

Best ever lecture..👌

[@nrsm305]

man thank god i found u thanks a lot :) you are the one

[@aldobhamomo6657]

finally I understand it, many thank you so much

[@arunkumars6257]

Thank you so much!

[@amisheriff709]

do you give private lecture??

[@huynhngan3743]

you are my savior :3

[@Axe10011]

But if that second solution of glucose replaces the first one, wouldn't the protein come out bound with the second solution, thus making it still not clear?

[@Axe10011]

I don't even know what the hell I was trying to ask 2 years ago.

[@trevorjefferson4840]

lol your were just saying that the enzyme was still bound to glucose and therefore it doesn't make since that we would call it isolated.

[@arungs2645]

I think the second solution should not be glucose, but some other molecule that has more affinity to the glucose attached to the bead, so that it will competitively bind to it making our protein/enzyme of interest free so that it moves down.

[@shmuelsash]

What's the answer? Is it bound?

[@MasayoMusic]

Axe10011 Actually it makes sense.

[@apollokabukuru6506]

Amen. Thank you!

[@geokyen9713]

hi, how do we know when it's time to collect the respective eluate? as in real life, the solution would be all same in colours.

[@surajsawantarts]

Thanks Sir.

[@casualcasual1234]

may I ask: 1. what can we do to ensure the enzyme will not digest, only binds with the immobilized substrate during running the column? 2. after step E, what methods can be used to extract the enzyme of interest i.e. separate them from the glucose solution and transfer them to a suitable buffer solution since there still exists a binding force?

[@cepecul]

You the MVP

[@misan1872]

omg i love u so much ! thank you!

[@naseerahmad3675]

so saweet u

[@levelone7511]

你好中国女人

[@mohamedhamdy4981]

Will you make lectures for informatics?

[@amalatif4097]

Thanks

[@amisheriff709]

thank you

[@zachariasshark]

Hi AK, can imidazole essentially do the same thing as pouring glucose to elute the protein of interest?

[@junczhang]

thank you ^_^

[@mbhuiyan223]

@AK Lectures... What if our protein didn't have color, how would we be able to separate then?

[@levelone7511]

Mahmud Bhuiyan through SDS PAGE electrophoresis.

[@shini5478]

hi, what does crude mixture of proteins mean?

[@PoetryCornerbyTSCollections]

Crude mixture, which has unknown sample whether proteins or other compounds

[@VijayKumar-nz2sj]

nice

[@aldobhamomo6657]

can you explain Gas chromatography please

[@AKLECTURES]

***** i already did a lecture on that.

[@naaznaaz6429]

very informative lecture ,,, thanks very much to represent such a grear lectures

[@avedesco]

Blue-green colorblind? Good lecture though.

[@AKLECTURES]

avedesco You telling me green-blue colorblind tells me absolutely nothing. If you want me to correct something I might have said in this lecture, add an annotation to your comment so I can go straight to that point and make the appropriate corrections.

[@naseerahmad3675]

avedesco so well

[@PLF...]

Dude the hair... Let it go.

[@shmooveyea]

You shut your mouth.