Automatic cell-annotation for single-cell RNA-Seq data: A detailed SingleR tutorial (PART 2)

[@pshubhamoy21]

This is a very useful video. Thanks for educating us. It would be a great help if you please make a tutorial about how to create a custom reference dataset. Thanks!

[@ahmedal-mammari9639]

i waited for your videos for a long time 😊😊

[@cissytabingwa2925]

Hello thanks so much for these excellent videos. Could you kindly make a video on GSEA in r

[@elafd5043]

Thank you for your great videos, could you please let us know how we can label our clusters manually...

[@natureabioros8686]

After 31:30, how do you write a column of the meta data that matches cell type to ontology ID?

[@yinxia-nl5ky]

Very helpful!!!Thank you very much!!😊😊

[@roddik160036]

Your background information are very detail and meaningful. Thanks for making it easy for biologist! Cheers B-magician! The last 7 mins is not clear: Why do we need ontology term instead of label.main? Looking forward for next video on the actual output of singleR for results figures.

[@nancunjie4560]

very awesome video! is singleR capable for the sub cell type classification? or what is your recommendation for classify the sub cell types?

[@余长]

Wonderful work! Your tutorial is clear and efficient! How do we understand the metadata(com.res2$orig.results$DICE)$de.genes? This is a list, and for each cell type, there is a list of all other cell types as well. It's confusing.

[@dr.hanyghazal3003]

Hi, doctor..I really appreciate your great content in teaching bioinfo.. Please I need a source for molecular docking and drug design expected to be as great as your content.. Thank you.

[@余长]

Using Strategy 3 to annotate, the two reference files are still having different ont names. Thus as a result, "B cell" and ''B_cell" are still both present in the final UMAP. I don' t think this is what you want, is it?

[@뚱카-y2t]

can i have some plant related species too?