Basics and principle of Fluorescence & Phosphorescence measurement | Learn under 5 min | AI 06

Analytical Instrumentation - Fluorescence & Phosphorescence measurement
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You may have come across terms like fluorescence and phosphorescence. You may have a lot of questions in your head regarding this topic like: What does fluorescent mean?, How does fluorescence works?, How does fluorescence spectroscopy work?, How does fluorescent occur? You will find answers to all these questions in this video.
In the following video, viewers will learn about the basic principle of fluorescence and phosphorescence. The generation of fluorescent and phosphorescent emissions is discussed in detailed using an energy level diagram of a molecule. In the energy level diagram we can see different levels of molecular energies. The electrons when in pair are either in singlet state or triplet state. The basic definitions of singlet state and triplet state is covered and pairing of electrons and their spins is explained in the animation. Once we understand the energy levels corresponding to singlet and triplet state, it becomes quite clear to understand fluorescence and phosphorescence. The fluorescence and phosphorescence emitted by a sample under test is used to perform qualitative and quantitative analysis on the analyte. It is performed using spectrofluorimeter. The basic principle of measurement of fluorescence is also explained in the video.
At the end of the video, viewer will also have a clear idea between the differences in fluorescence and phosphorescence and will be able to compare the two topics.
Hope you like and enjoy the video. Give us a thumbs up. Stay calm and keep learning. Subscribe to the channel to learn about such more interesting concepts and to never miss any new updates.
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[@PracticalNinjas]

Get amazing content and support on Academic Courses, GATE Courses, Professional Courses and Career with Ekeeda. Go to ekeeda.com/ platform and register with the referral code EAPHAR74. Use the coupon code Ninja10 on purchase for special discount! We have an exciting news for everyone! We have developed a course on PLC basics in collaboration with Internshala where you can get good discounts using the coupon code ISRP16947850. Go check it out here- trainings.internshala.com/plc-programming-training

[@bosong2515]

I really like the video but I would like to suggest that you remove the background music. It is distracting!

[@PracticalNinjas]

Thanks Bo Song for your valuable suggestion. We are glad you liked the video. We will try to remove the background noise or atleast reduce it's volume level in our future videos :)

[@vrajpatel429]

No back ground music is good I like it it effective

[@sanhitamarjit895]

Exactly,why the hell is background music there

[@jermiinus]

@@PracticalNinjas Would you please reupload this video without the background music?

[@PracticalNinjas]

Due to certain technical issues we are unable to upload the video without background noise

[@vinayegalapati2191]

You're explanation and PPT was awesome 😍 But, Please decrease background volume I'm unable to concentrate on the concept

[@Alex-vs8ev]

Man, I'm from Germany. And idk what the hell is going on with our teachers but I understood this topic better in not even 5 minutes than in 2 weeks of school lessons. And your video is in English !!!

[@soumyamuttagi]

How I couldn't find this video earlier. Really amazing and beautiful explaination. Finally I can score and understand at the same time 🙌🙌🙌thank youuu

[@cherryberry6492]

Good explanation.But music is make concantrate difficult.

[@PracticalNinjas]

+cheery berry. Thanks for the feedback. Will try to keep music volume low in the next videos 😃

[@cherryberry6492]

Thank you for your consideration :)

[@PracticalNinjas]

Hope you enjoy other videos on our channel as well :)

[@ACswe91]

Yes keep the music but little lower :)

[@PracticalNinjas]

Sure ACswe91 :)

[@minhavidavai]

This was great. I needed to remember these concepts in short time. Thanks

[@whiteboardanimatorhamna]

Beautifully explained... Thank u for making it quite easy to understand!

[@user-fd7hy9ko3h]

The 1st second I was like "music😫", but then I was into the mood,thank you so much, I was having real trouble with singlet and triplet state 🌺

[@LadyShaydex]

I think you should add why fluorescence light is higher in wavelength, due to energy being lost to vibration

[@gameon4195]

Higher in wavelength dear please correct

[@LadyShaydex]

@@gameon4195 Lower in wavelength to the incident light is what I meant

[@LadyShaydex]

@@gameon4195 Oh wait you're right

[@gameon4195]

@@LadyShaydex thanks 😊

[@TestEric]

WOW, amazing job, this is exactly what I was looking for. I'm shocked more people are not interested in this topic.

[@harshitabhati4747]

Awesome!! I can never forget the concept after watching this!!

[@PracticalNinjas]

Glad you liked the video Harshita Bhati :)

[@chitrakeshwani4047]

thank you so much for making this video you have explained this phenomenon in a very simple and comprehensive way

[@PracticalNinjas]

Thanks Chitra Keshwani for such a positive feedback. We are glad that you liked the video!!!

[@KR-jz9xv]

Thank you so much for a very clear and straightforward explanation!

[@PracticalNinjas]

We are glad you liked our video Khawllah Roussi

[@ncarti1601]

Great video, really liked the visuals and it helped me understand it more!

[@amitvele]

Excellent work. Colorful and creative. Makes the video engaging.

[@PracticalNinjas]

Thanks +Amit Vele.

[@savitact7960]

thankyou. very well explained. it would me better if no background music.

[@PracticalNinjas]

Thank you +Savita Ct for the support and feedback. The background music in the future videos will be kept to a minimum so that it does not distract the viewers from the main concept :)

[@yasinbaraqouni9395]

Agree with you

[@leif1075]

@@PracticalNinjas why is it called triplet state instead of doublet which makes more sense since there are two?

[@leif1075]

@@PracticalNinjas Thanks for the video. I actually liked the background music..Could you tell me who the composer is?

[@safwanahrahman4109]

simple explaination and i understand the whole thing better!!! THANKYOU

[@PracticalNinjas]

Thanks Safwanah Rahman for such positive words.

[@pdiala2]

Perfect explanation. Everything I was looking for

[@PracticalNinjas]

Glad we could help you :)

[@kashishkanodia5629]

Thankyou for the simplified explanation!

[@dariyatukhmetova1172]

this video is amazing, thank you vvery much

[@Smoothcurveup52]

Thank you so much sir Superb explaination

[@sjoerdklomp3931]

Thank you this helped a lot i have to make a oled from Ru(bpy)32+ and this helped me learn some of the basics

[@PracticalNinjas]

Happy to hear that the video was helpful :)

[@innocentchemist2556]

Good work. it was really helpful for me. this video cleared my basic concepts!!

[@hceperepnoname8752]

Cool!Thank you so much!

[@paritoshsharma2841]

Thanks a million !!!! Nice explanation . Keep it up guys 👍

[@PracticalNinjas]

Thanks Paritosh Sharma for the support :)

[@cartikey3743]

@@PracticalNinjas but bro my question is why that Intersystem crossing takes place

[@vrajpatel429]

Very nice discription very good way to explained keep it up

[@sukanyaghosh6503]

Beautifully explained!!!

[@PracticalNinjas]

Thanks Sukanya! Glad you liked it.

[@kimsd6269]

thanks you for your efforts, Indeed.

[@annurukay7040]

Asome sir u define it very easy to understood 👌👌

[@mahnoor6216]

Thanks for clearing out concepts 😊

[@rabiyanoor4459]

Tremendous explanation... Helped me alot

[@01244070178]

Nice video sir. Excellent explanation 👍

[@mdasifansari2061]

Very nicely explained 👍👍.... Thank u so much

[@missdaydreamer6777]

Helped me a lot. THANK YOU! Suggestion though, remove the effect on the texts. :)

[@PracticalNinjas]

Thanks a lot for your feedback :)

[@ketitzudi8495]

understandable.Keep up the good work

[@PracticalNinjas]

Thanks +ketiyala tzudi. Glad you liked it

[@torlarsen2212]

Amazing video sir! Thank you so much

[@malaneyy408]

This was so good thank you. Agree with others about removing the music though.

[@sowjanyaponnada7234]

Nice and telugu explanation... Is better to understand

[@tehminashafique74]

It was so simple and easy to understand thank u

[@chaski315]

Overall this is a great video. Thank you!

[@laszlokecskes8072]

well done! thanks!

[@tejasshewale953]

This explanation is really beneficial ...

[@bushra6319]

Best 👍 jazak Allah Khair

[@jesper1320]

does this jumping on and off of energy states mean changing orbitals ? or something different ?

[@3ashmawy660]

useful and easy to understand explain . thanks

[@3wordsoxforddaily786]

thank you so much Now I understand

[@PracticalNinjas]

we are glad the video could help you :)

[@kundaiashleychakona1565]

This helped me a lot! Please edit out the music.

[@nallasurenderreddy6355]

Thank u Helped a lot

[@pratikadarsh6894]

Very helpful. Thanks

[@snehithatalla7018]

Gud job ...keep going👍🏼

[@PracticalNinjas]

Thanks +snehitha talla. Glad you liked our work.

[@abhiriseagain9303]

Thank you so much❤️❤️

[@vineetkhantwal9410]

Awesome 🤗🤗🤗

well done thanks.

[@PracticalNinjas]

Thanks +Furkan CAPRAZ. Glad you liked the video.

[@sumonagarg3428]

Very helpful Thankyou so much

[@mohanrommala8252]

really simplified explanation

[@hariom9328]

I appreciate the efforts of making this video with intention to simplify the concept. However, i noticed that you have forgotten to mention several things including the internal conversion, types of molecules that can fluoresce etc. Another thing is that you have wrongly mentioned the lifetime in the singlet excited state as varying from 10^-8 to 10^-4 seconds. These l lifetimes are I few nano seconds only.

[@prakashjoshi6796]

Hi, Where do you make this videos, in which software

[@sidewaysfcs0718]

The Jablonsky diagram doesn't depict orbital energies, but the energy states of the molecular electronic system as a whole. You cannot put electrons on this diagram like you would in a molecular orbital diagram. Basically, every energy level in a Jablonsky Diagram represent its own personal molecular orbital diagram which is omitted.

[@nishadkaippally8247]

The background music adds so much meaning to this presentation. Perhaps more volume could enhance our understanding.

[@yasaine7896]

music volume down or a more no music would be better, but excellent video and very easily understandable, awesome job.

[@PracticalNinjas]

Thanks +Yasmine Bingei for the feedback :) Will surely try to reduce the volume in the next videos. Glad you liked the video.

[@ELLE-zr3cs]

awesome music!and explanation

[@19arathiv49]

thank u so much

[@lonehumi8350]

Thanks a lot sir

[@PracticalNinjas]

Glad you liked it

[@somoprovahalder1819]

Sir,thanks a lot for your video... Can you please tell me , that how will I know , whether a given compound is capable of producing fluroscence or not ?? For example, say acetone... Will it show fluroscence ?

[@lokeshgnanasekar]

Acetone may fluoresce when a high intensity monochromatic sources are made to fall incidentally. It is difficult to analyse whether all compounds fluoresce or not, since we consider that phenomena generally in the visible spectrum; What if it emits in invisible wavelengths...Hope you know that's why we use UV/Vis spectra, thought many paper suggests they can identify, it has many limitations Edit: For vibrational energy we use IR

[@LOADEDUPTV]

Great content

[@kaveeshasandeepani6105]

How does the wavelength increase after

[@princessprincee3265]

Nice

[@muhammadshuaib406]

very helpful for beginner

[@NavneetPannu-qu9hv]

sir please tell why the emission wavelength is greater than the absorbance wavelength ?

[@kiranyadav9782]

Thanku soooo much sir 🙏🏼🙏🏼🙏🏼🙏🏼🙏🏼

[@raeesladduawesomebhaiya1429]

Thanks sir

[@PracticalNinjas]

Glad you liked our video :)

[@Gopi_6094]

supper...b i really like this video

[@Vedrajrm]

The isc should happen horizontally, so to a same energy level From there due to vibrational relaxation it comes to T1

[@savitact7960]

thankyou. very well explained .it would be better if no background music

[@PracticalNinjas]

Thanks +Savita Ct for the feedback. We will surely try to reduce the background music.

[@somebody9824]

Voice of narrator is really good

[@nerym.aguilar4297]

You are amaziiiiiiiiing!!!

[@meerasubu1788]

Great explanation, I ave a quert why is excitation of radiation at 90 degree

[@Rajput0818]

Nice one thanks

[@PracticalNinjas]

:)

[@dwipayanhalder4388]

Good explanation

[@RaniYadav-gx4yt]

Nice video sir

[@mauroariascontreras9284]

Nice video man =) and I din't have troubles with the background music... actually I found it nice hahah poor little children crying

[@PracticalNinjas]

We are glad you liked the video :)

[@Ufaqunnisa]

Can u plz upload video on franl condon principle ... Its urgent.. Thank u sir

[@PracticalNinjas]

The channel is majorly dedicated to engineering videos. The Frank-Condon principle is a part of quantum chemistry where we have no expertise. Still we will try to understand and if possible upload a video on it. Thanks again for the approach :)

[@user-vu6kp9pp9b]

Perfect

[@sumonroy3766]

Is it molecular transition?

[@bugzy9476]

Good explanation..keep it up

[@sheikhfahmi6810]

nce vedio thanku

[@PracticalNinjas]

We are glad you liked our video.

[@muskan2486]

👍

[@anupamajoy199]

Sir, why phosphorescence and fluorescence always happens from first existed state

[@sayanjitb]

What is stray light radiation then at the end of video?

[@atvoriginals]

_Awesome_

[@piyushsen7502]

Thnx. 💗U

[@faisalwighral8647]

very well explaind

[@PracticalNinjas]

Thanks Faisal Wighral

[@sumonroy3766]

Molecular transition to higher electronic state....I didn’t catch that... Is it molecular transition??

[@m.skumar147]

superb..

[@PracticalNinjas]

Thanks +Shravan Kumar Muppidi. Glad you liked the video.

[@saadrashidusmani832]

love the video maker

[@bablykhatun3928]

Good content but In study videos try not to use background music

[@pratapGk21]

How to Calculate Arbitrary Units and fluorescence intensity?

[@nutansingh5194]

Plz tell about atomic absorption spectroscopy

[@PracticalNinjas]

We had uploaded a video on the topic you asked. But due to some technical doubts we have pulled that video down. Once those doubts are cleared, we will publish the video again.

[@mdkhabeerazhar216]

thanks

[@PracticalNinjas]

Glad you liked the video.