Beer Lambert's Law, Absorbance & Transmittance - Spectrophotometry, Basic Introduction - Chemistry

This chemistry video tutorial provides a basic introduction into spectrophotometry and beer lambert's law also known as beer's law. It briefly explains how a spectrophotometer works. It uses a light source and a monochromator which selects a specific wavelength of light using a prism or a grating. The amount of light striking the sample is the incident light Io and the intensity of light picked up by the detector is I. The ratio of I to Io is known as the transmittance. The absorbance is the negative log of the transmittance. As the transmittance increases, the absorbance decreases. Beer's Lambert law is associated with the equation A = elc where A is the absorbance, e is the molar absorptivity, l is the pathlength in cm and c is the concentration in Molarity or moles per liter. This video contains plenty of examples and practice problems calculating the absorbance, molar absorptivity and unknown concentration using beer lambert's law. This video explains how to determine the linear equation in slope intercept form given a data table of absorbance and concentration. It also explains how to determine the unknown concentration using proportions.
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chlorophyll amolar mass of 902.5 g/mol absorbs strongly a 660 nm. At this wavelength it was found that a chlorophyll asolussion in acetone of concentration 11 ug/cm^3 in a glass cell of length 1.00 cm transmitted 12.5% of the incident light What is the concentration of chlorophyll in mol/L. Determine the molar extinction coefficient for chlorophyll. Solution for this problem?

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A sample of K2SO4 of total mass 40.7 g contains KMnO4 as an impurity. The molar absorptivity E of KMnO4 is 1.80 ⇥ 104 L mol1cm1 at 520 nm. The sample is dissolved in 5.00 ⇥ 102 mL of water. Then, 2.00 mL of this solution are placed in a cuvette with 1.00 cm optical path and a UV-Vis absorption spectrum is recorded, displaying a maximum at 520 nm with transmittance value of 18.6%. Calculate the mass percentage of the KMnO4 impurity present in the initial sample, assuming that K2SO4 does not contribute to any absorption at 520 nm.

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[@kwon_shihooo]

I was confused a little bit. for the letter b question, shouldn't i recalculate molar absorptivity with the new given molar concentration (0.65M) as well, before I proceed to the absorbance computation?

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I love this video...❤❤❤ the transmittance of a solution is found to be 35%. what is the transmittance if the solution is diluted by half? Please help with this homework...

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[@eugenemacauley3206]

Thank you very much for this. God bless you But I want to ask...what if in another calculation, you don't get the y-intercept to be zero? That will make it a problem in relating the absorbance relation with the line relation. How do you solve that problem?

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Why slope and intercept are important? Can you show how to calculate slope and intercept?

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[@gauperoehrs7775]

Hello! For my physics class, I had to do an experiment to demonstrate Beer's law. I used a light sensor which gave results on lux (unit of measure for iluminance). Would it be correct to use measurements in lux instead of W/m2 to calculate transmittance? I'm thankful for any help I can get :)

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Laser radiation with a wavelength of 720 nm hits a homogeneous surface with a power density of 1 W/cm² Medium. After penetrating 10 cm into this medium, the radiation has only one intensity of 0.904 W/cm². Which medium is it? can anyone please guide about this equation?

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If I have A and I wanna find T, how do I calculate ? T=-log A?

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1. An analyst determines the concentration of potassium in five replicates of a standard water sample with an accepted value for its potassium concentration of 15 ppm by Flame Atomic Emission Spectrophotometry technique. The results he obtained in each of the five analyses in ppm were: 14.8, 15.12, 15.31, 14.95 and 15.03. Classify the error in the analysis described above for the determination of potassium in the standard water sample as determinate or random. Please help me

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A determinate error can be further categorized into two: constant determinate error and proportional determinate error. Constant determinate error (ecd) gives the same amount of error independent of the concentration of the substance being analyzed, whereas proportional determinate error (epd) depends directly on the concentration of the substance being analyzed (i.e., epd = K C), where K is a constant and C is the concentration of the analyte.

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In what units do you measure intensity?

[@2-hons-chem221]

Express the following absorbance value in terms of percent transmission 0.765 How can I solve the problems?please

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I have a doubt. If I want the absorbance of a compound trapped in a micelle, will the micelle affect it?

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Why would you not have to recalculate the molar absorptivity for part B?

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Problem 2

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[@M-baku]

Question on part 13:17. How do you find concentration when you only have the slope and y-intercept for y=mx+b?

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You got the relation between Absorbance and Concentration. So, use that to solve for values of either Absorbance or Concentration.

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so the molar absorptivity will still be constant if we change the concentration ?

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Can someone epxlain to me why is it that Absorbance and Transmittance don't sum up to 1 please? D:

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is the absorbance of water 0?

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