Benedict's test- for reducing sugars

[@tanay2143]

Principle: Reducing Sugars under hot alkaline condition undergoes tautomerization into enediol form. Enediol are strong reducing agents and convertscupric ions of the Benedict'ssolution into cuprous ions. The cuprous hydroxide formed during the process of heating is converted to cuprous oxide as red precipiate.

[@DenimDeni]

This is very big sentence

[@itsmuaz749]

What will happen if we dont heat it? Will it still change colour

[@04.d0lly]

Thank you so much....

[@lolsuprise5094]

@@itsmuaz749 nope

[@mubasharali8064]

p

[@attulurivenkatabadarinath1044]

Clean sweeep... Covered all points.. It seems... Your student is sincere, obedient than my nouty students..

[@Rajesh_Jambhulkar]

😀

[@Anush_Sivakumar]

lmfao teacher

[@Lilygirl6085]

Lol

[@MadridTV12]

Nouty 😂😂😂😂😂Rip english 😢😢Rip Such fool teachers 😢😢😢

[@pharmatricksforexams5967]

@@MadridTV12 hi... Its me... Badri nath... I wrote sweeep also mistake.... Not observed?

[@muzammilmaqsood3484]

Thank you for your effort. Please keep uploading videos of more biochemistry test. Thanks

[@rishipramanick6823]

Wow its exactly written in satyanaryana Cupric is reduced to cuprous and color is cuprous hydroxide yellow and cuprousoxide red Another good to test is osazone reaction Which involves phenyl hydrazine

[@zarishiftikhar5520]

Nice explanation...! You Are The Best ! ❤❤❤ ❤❤❤❤ ❤❤❤❤❤❤ ❤❤❤❤❤❤❤ ❤❤❤❤❤❤❤❤ ❤❤❤❤❤❤❤❤❤❤

[@nitingupta8818]

Very helpful sir by the way I m not MBBS student, I am From Paramedical (MLT) field & sometimes I need to watch your lecture sir & pls sir upload a more Practical video such as it is Benedict's test Thanks sir

[@insightfulmotivebyzehra2309]

In which clg??

[@nitinkumargupta635]

@@insightfulmotivebyzehra2309 Sanskriti University

[@thesleema6975]

Iam also a MLT students

[@insightfulmotivebyzehra2309]

@@thesleema6975 kis University se ho?

[@faizan7343]

Tatty university

[@viveksurwase499]

Thanku u for making videosa on practicals such a useful video 🙏🙏thanku so much great work

[@ahfaz6183]

Best video...make more video of test sir best

[@easytoolsupported]

Sweet voice and good experiment perform

[@advikninghot4430]

Very good.. Keep it up

[@benazeerfatima5883]

Very helpfull ,plz upload more practicals of biochemistry

[@kunalsingh2943]

Thanks Archana ma'am 💓💓💓💓💓💓

[@priyanshgupta700]

Beautiful result...... Thx sir...!

[@crumbforducks]

Does the solution turn green *to* brick red *after* cooling? I feel green ppt was due to less concentration of the reducing sugars in the solution, and brick red due to higher concentration. Right? Well explained👍

[@gunachandhiran3345]

Depending upon theamount of reducing sugar in the urine the color will deffer such as green(+),yellow(++),orange(+++) and prik red(++++)

[@kunalsingh2943]

Thanks dikhsha 💓💓💓

[@DrFaisalRajpoot]

❤❤❤❤❤ good 👍

[@jawairiasherazi5127]

seriouly!!! waterbath is used for heating so the heat spreads equally its so dangerous to shake the tube like that it can also spill

[@sgamingyt2122]

Thank u for your wonderful vedio.nyc explanation.

[@pharmasolutionpakistan7668]

Good Informative

[@satyambhola5187]

Awesome sir 👏 🙌 👌

[@Ruba-v2y]

Can we use water bath to heat in all the test of carbohydrates...i think result will be proper and easy to come..if we don't use in some test the soln is coming out of the test tube hence creating difficulty to get results

[@Rajesh_Jambhulkar]

Yes

[@shahzadhussain3146]

Please upload more videos on practicals

[@sajidaparveen6680]

Outstanding explanation

[@adams4807]

Useful

[@bhartiyadav591]

Sir if I learn nicely all ur vedios can I score well in biochem in proff?

[@drrsmishra1642]

Nice video 😊

[@hana7812-l2l]

To observe the colour change clearly it should be immersed in a water bath and heated.

[@Shiraz321]

i am going to buy test tubes .

[@fastlearningclasses330]

Home sugar positive test?

[@rc-ul7pt]

Thank you so much sir😊

[@jashalawrencin6795]

what was the sample used?

[@UdayRaj-mz5sf]

Urine sample

[@fastlearningclasses330]

Milk as a sample le to positive test hoga?

[@navitameena6832]

Or video upload kijiye sir ji practical k liye

[@tajudeenfausiyat9130]

Keep it up

[@PWagan]

What will the result be if you tested a tomato and if you tested a cucumber. Please help.

[@deepshikhasoni8019]

What is the name of this test particularly?

[@techtimes1985]

Good

[@Quick-flick05]

Why there in need to convert cuprous hydroxide into cuprousoxide form by heating??

[@aadamkhan5217]

It’s a pipette not a droper

[@Dryesno2]

Pasa marra

[@ulisessilva1814]

Can you use some kind of catalyst for this method , and deposit that copper on som surface ,or exsample gripping a copper mirror?

[@rishipramanick6823]

U are carrying it in alkaline medium is any catalyst further required

[@pratikbhiungade858]

👍🏻👍🏻🙏🏻

[@alienalien6577]

Name of the sample added?

[@Josh-yl1it]

👌👌👌👌👌👌👌

[@AryaRathi-b1g]

Mam if while heating the colour doesn't change but after 15 minutes you get red ppt settle at bottom, then will it be reducing sugar

[@learning-pe9ww]

👍👍👍

[@pushpendrasharma4669]

Sugar can be tested in a diabetes patient by। Benedict's test or barford test

[@ayanhalder5280]

👏👏👏👏

[@Abhay001_]

Thank you !

[@MirabZia-vt9xh]

Thanks a lot

[@deepasaha499]

Provide other exp too

[@HashaamShami-nd9tg]

❤❤❤🎉🎉🎉🎉

[@sdiwakarg694]

Sample means I mean kesa sample what we can take in it

[@anurakumara7305]

Sir if we have to recognize non redusing sugar and reduisng sugar which are in one solution ,how can we identify them separately?

[@Abdullahkill3333r]

you would seperate the sample, and test both separately. as far as I know - there's no way to actually test for non reducing sugars and reducing sugars within one test tube

[@aahilzaman6682]

If the Benedict’s test gives negative result then we have to slightly heat it using hydrochloric acid and then let it cool down.Then add an alkali like sodium hydrogen carbonate to break down the glycosidic bonds in the non reducing sugar and then carry out the test.

[@RajibGhosh01]

Need Benedict test for making assigment

[@ala7585]

If Benedict's test shows blue at the start, dont we already know its non reducing, so why do the things after? Y is hcl used instead of water to break down sugar?

[@chosnotes162]

i think it's to determine the concentration

[@velocimer]

Why don't u sue thermostatic controlled water bath

[@KevinRaj-w7z]

Do you do online teaching sister?

[@nooraisyahahmad8879]

Hi, may I know the benedict solution will reacts faster in less or more concentrated glucose solution?

[@rishipramanick6823]

More In alkaline medium

[@Hinakhan-oj8nm]

Why heated alkaline is used

[@rathnakararrathnakara3191]

Why should we add only 8 drops of urine because it is a semi quantitative the thing is what happens if we add more drops of urine or less ?

[@sumithrashankar9072]

If u add more .the non- carbohydrate which have a reducing property gives false positive for Benedict test .

[@husainarwaz8822]

Please tell the composition of benedicts reagent

[@ashkethum4876]

It's given in video when Benedict agent was mentioned

[@prathmeshteli3057]

Benedict's reagent react with reducing sugar color get yellow,.why? give the scientific reason easy to understand...

[@Rajesh_Jambhulkar]

Reducing sugar in hot alkaline medium forms strong reducing agent Enediol. This reduces cupric ions to cuprous form. There is formation of Cu(OH)2 and CuO2 which gives different colors dependent on sugar concentration.

[@shahriarkawsar5526]

Thanks mam

[@channabasappaangadiyavar8821]

👌👌👌👌👌👌👌👌👌👌👌👌👌❤️

[@ultimateones2990]

How to make sample

[@durjay21]

when auto analyzer was not there how pathologists measured blood sugar?

[@Rajesh_Jambhulkar]

By manual methods on colorimeter

[@iramshazadi1503]

Sample Kya cheze ha?

[@shadilhassan6890]

What is sample

[@nutritalks9938]

What are these gm%? Is these amounts for glucose?

[@Rajesh_Jambhulkar]

gm/dl

[@nutritalks9938]

Gm glucose present in 100ml solution?

[@RutendoChangamire-ce2om]

Please next time use a water bath

[@imanabass5660]

Rajesh koothripali

[@imanabass5660]

Oh my I remember him from Big Bang theory

[@imanabass5660]

Same

[@imanabass5660]

Me too

[@shotfox6371]

This is sad

[@dakshindarshanbalaji6007]

What is the sample mixed of

[@Sreekuttyvishnu1999]

Urine

[@joleyreliquias2547]

Does a non-carbohydrate substance be positive in Benedict’s test?

[@man-mk2bx]

Nope

[@crumbforducks]

No, maltose and glucose are types of sugars- carbohydrates. Non-carbohydrate substances will definitely not have these two reducing sugars present. The solution will remain blue (CuSO4- Copper Sulphate).

[@sumithrashankar9072]

Yes.Non_ carbohydrate like uric acid,ascorbic acid ,glutathione give false positive for Benedict test

[@bendovrsenpai9582]

What is gm%?

[@Rajesh_Jambhulkar]

Gm/100ml

[@payeldas7853]

Any pdf

[@BrightHero_Ultimato]

اختبار عملي

[@Ragoo-ht]

Freshy 😂😂😂

[@msthasminnaharhumayra5143]

It is not right method.

[@Ian-ei7ee]

DILI MAKA CHECK OUT. IF DI MAKA TAN.AWS VIDEO HAHHAAH

[@gws8525]

Hari bokkala