Urine Analysis for Abnormal Constituent | Sugar Test by Benedict's Reagent in Urine | यूरिन सुगर

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Also available in ENGLISH. Determination of Sugar in Urine = Benedict Test (Pathology Experiment)= ENGLISH By Solution Pharmacy • Determination of Sugar...
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Basic Cause and reason for Diabetes- • Diabetes Mellitus | Ca...
Diabetes mellitus is a disorder in which blood sugar (glucose) levels are abnormally high because the body does not produce enough insulin to meet its needs.
Type 1 Diabetes Mellitus
In type 1 diabetes (formerly called insulin-dependent diabetes or juvenile-onset diabetes), the body's immune system attacks the insulin-producing cells of the pancreas, and more than 90% of them are permanently destroyed. The pancreas, therefore, produces little or no insulin. Only about 5 to 10% of all people with diabetes have type 1 disease. Most people who have type 1 diabetes develop the disease before age 30, although it can develop later in life.
Type 2 Diabetes Mellitus
In type 2 diabetes (formerly called non- insulin-dependent diabetes or adult-onset diabetes), the pancreas often continues to produce insulin, sometimes even at higher-than-normal levels, especially early in the disease. However, the body develops resistance to the effects of insulin, so there is not enough insulin to meet the body’s needs. As type 2 diabetes progresses, the insulin-producing ability of the pancreas decreases.
Complications of diabetes
(1) Brain, causing a stroke
(2) Eyes (diabetic retinopathy), causing blindness
(3) Heart, causing a heart attack
(4) Kidneys (diabetic nephropathy), causing chronic kidney disease
(5) Nerves (diabetic neuropathy), causing a decreased sensation in feet
Benedict’s solution is a deep-blue alkaline chemical reagent used to test for the presence of the aldehyde functional group -CHO which consists of copper sulfate pentahydrate (CuSO4. 5H2O), sodium carbonate (Na2CO3), sodium citrate (Na3C6H5O7) and distilled water. Sodium carbonate renders alkaline conditions which are required for the redox reaction, while sodium citrate is a complexing agent which complexes with the copper (II) ions to avoid degradation into copper (I) ions during storage.
Principle of Benedicts test
Benedict’s test is performed by heating the reducing sugar solution with Benedict‘s reagent. The presence of the alkaline sodium carbonate converts the sugar into a strong reducing agent called enediols. During the reaction, enediols decrease the cupric particles (Cu2+) present in Benedict’s reagent to cuprous particles (Cu+) which appear as red copper oxide (Cu2O) which is insoluble in water. When Benedict’s reagent solution and reducing sugars are heated together, the solution changes its color to orange-red/ brick red precipitate. The red-colored cuprous oxide is insoluble in water and hence, separate out from the solution. When the concentration of the reducing sugar is high in the solution, then the color becomes more intense (reddish) and the volume of the precipitate increases in the test tube making it clearly visible.
The procedure of Benedict’s Test
1. Pipette out 2 ml (10 drops) of Benedict’s reagent and placed it in the clean test tube
2. Approximately 1 ml of sample (urine) is added to Benedict’s reagent.
3. The test tube is placed over the boiling water bath for 3-5 minutes (can be heated directly over flame).
4. Observe for color change in the solution of test tubes or precipitate formation.
Result Interpretation of Benedict’s Test
1. If the color upon boiling is changed into green, then there would be 0.1 to 0.5 percent sugar in solution.
2. If it changes color to yellow, then 0.5 to 1 percent sugar is present.
3. If it changes to orange, then it means that 1 to 1.5 percent sugar is present.
4. If color changes to red,then 1.5 to 2.0 percent sugar is present.
5. And if color changes to brick red,it means that more than 2 percent sugar is present in solution.
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Пікірлер: 132
@28vimalkumarsahu90
@28vimalkumarsahu90 2 жыл бұрын
Koun koun exam se pehle meri trh pdhne aya h🤗😋
@priyadongre9366
@priyadongre9366 2 жыл бұрын
Mai kal exam hai
@Ahianworld
@Ahianworld 2 жыл бұрын
Mera kal
@saradagoswami4065
@saradagoswami4065 Жыл бұрын
I have exam tomorrow 😂
@saradagoswami4065
@saradagoswami4065 Жыл бұрын
And I am making my first time and last time preparation through these vedio😂😂
@nargisparveen7872
@nargisparveen7872 Жыл бұрын
Me
@xlisten9172
@xlisten9172 2 жыл бұрын
Thank you MAM.... it's totally Amazing... Tomorrow is my practical exam i was thinking i can't.... But seriously u make it very very easy.... Tnku so much 💗💗.... Love from Kashmir 💗
@pratapkumar4852
@pratapkumar4852 2 жыл бұрын
Hi
@Hitzz78
@Hitzz78 2 ай бұрын
Same thing😂
@jabasaharia5813
@jabasaharia5813 11 ай бұрын
Watching this video before 1 hour of exam😅
@dhairyashilshinde4578
@dhairyashilshinde4578 5 ай бұрын
@@jabasaharia5813 😂😂
@AbdulAwaiz-f3x
@AbdulAwaiz-f3x 4 ай бұрын
😂😂😂
@MohitPATEL-pd9sy
@MohitPATEL-pd9sy 3 ай бұрын
Same bhai 😂😂
@namratasahu8892
@namratasahu8892 Ай бұрын
@@jabasaharia5813 aur comment krne ka bhi time mil gya 😂
@Skkkkkkkkr
@Skkkkkkkkr Ай бұрын
😂😂😂
@SapnaMasih-rf7xm
@SapnaMasih-rf7xm 5 ай бұрын
Watching just before one day of my exam thanku mam
@shivangi3004
@shivangi3004 7 ай бұрын
Watching just before my viva
@odiastorytvodia
@odiastorytvodia 11 ай бұрын
Easily understood 😊 thank you ❤
@sujansheelsrivastava7420
@sujansheelsrivastava7420 3 жыл бұрын
No one can explain as good as you thank you so much mam 🙏🙏😌
@solutionpharmacy
@solutionpharmacy 3 жыл бұрын
Thank you
@totalarmy9628
@totalarmy9628 Жыл бұрын
✅Correctly
@sheetal5088
@sheetal5088 Ай бұрын
Watching during exam 💀
@faijalam3170
@faijalam3170 Жыл бұрын
I can't tell you how happy I am. You are explaining so good. ☺
@jayakaran.b4276
@jayakaran.b4276 Жыл бұрын
Video is very Good .But small changes has to be done in this test first we should heat the benetic solution only .why because means whether the benetic solution is expired or not we should check for that .we heat first benetic solution then after few minutes later add urine sample by using droper .
@solutionpharmacy
@solutionpharmacy Жыл бұрын
Thank you so much for your suggestion. We will keep thing in mind.
@shrishailgudodagi572
@shrishailgudodagi572 28 күн бұрын
Thank you mam
@md.saqlainmustak501
@md.saqlainmustak501 3 жыл бұрын
Superb same here 🤗
@Kishan__gaming74
@Kishan__gaming74 Жыл бұрын
Mam aap bohot achchese practical samjhate ho 😊😊❤❤ thank you
@ankitaraj_drdz
@ankitaraj_drdz Жыл бұрын
Amazing video U explain all the terms clearly n Sweetly 🤟✌👍
@solutionpharmacy
@solutionpharmacy Жыл бұрын
Thanks a lot 😊
@dr.medico8176
@dr.medico8176 Жыл бұрын
Kon kon exam k pahlay din padne aya hai
@Deep_blue49
@Deep_blue49 11 ай бұрын
Main 🙂
@medicaleducation8978
@medicaleducation8978 3 жыл бұрын
Lots of love from Pakistan ❤️❤️❤️
@NaveenAgrawal-k3y
@NaveenAgrawal-k3y Жыл бұрын
Thank you😊
@ytsonube
@ytsonube Жыл бұрын
very nice mam i very easily understood
@whatthezindagi6812
@whatthezindagi6812 Жыл бұрын
Thank you so much you explained it so well
@pardeepdhanjal9067
@pardeepdhanjal9067 3 жыл бұрын
5ml Benedict Solution add 0.5ml or 8 -12 drops of urine ok
@Motilalmahto739
@Motilalmahto739 Жыл бұрын
Right
@akashsurjahe4219
@akashsurjahe4219 Жыл бұрын
Thanku❤
@COMPARISON--GUY
@COMPARISON--GUY 5 ай бұрын
Watching this 20 mins before exam 😂. Thank you 🙏🏻😊
@the100thcreature
@the100thcreature 10 ай бұрын
Practical ke 1 ghante pehle dekh ra
@SangeetaBagh-fs2in
@SangeetaBagh-fs2in 8 ай бұрын
Mai bhi
@Baratang-xxx56
@Baratang-xxx56 9 ай бұрын
बहुत अच्छा
@iamindian8629
@iamindian8629 2 жыл бұрын
Thanks ❤️
@MousumiBoro
@MousumiBoro 10 ай бұрын
Thanks you❤
@asimsoomro4881
@asimsoomro4881 Жыл бұрын
Good explained
@gunasekaranr1843
@gunasekaranr1843 Жыл бұрын
Super mam thanks
@dr.chandrakantmagdum1735
@dr.chandrakantmagdum1735 3 жыл бұрын
Very good mam
@anamikasingh7068
@anamikasingh7068 2 жыл бұрын
Thanks for you
@TH.631
@TH.631 10 ай бұрын
5ml reagent me 0.5ml urine sample dalte he jiska ratio 1:10 hota h
@shamaparveen8789
@shamaparveen8789 Жыл бұрын
Can we take sucrose instead of glucose
@styledreams
@styledreams 2 жыл бұрын
Thank you 🥺❤️
@utsabkoner4907
@utsabkoner4907 2 жыл бұрын
Nice explain
@salehaam9267
@salehaam9267 2 жыл бұрын
Super 🥰🥰🥰
@salehaam9267
@salehaam9267 2 жыл бұрын
⭐🌟👍👍👏👏👌👌👌👌👌
@ananyasahoo8876
@ananyasahoo8876 2 жыл бұрын
A big thanks from my heart mam ,this is very help full
@THESHIV2.O
@THESHIV2.O 3 жыл бұрын
Good ma'am
@sateeshkumar-mw2kx
@sateeshkumar-mw2kx 2 жыл бұрын
Thank you somach medam.. 🤗
@VijayRajputMp39
@VijayRajputMp39 2 жыл бұрын
Mam alcohol fermentation per video banye.👍👍
@solutionpharmacy
@solutionpharmacy 2 жыл бұрын
Already uploaded
@Anju-k4
@Anju-k4 4 ай бұрын
Urine sample zayda liye hain apnay , its 8 drops or 1/2 ml if urine
@solutionpharmacy
@solutionpharmacy 4 ай бұрын
Its qualitative not quantitative
@Ashakumariblogsystem888
@Ashakumariblogsystem888 Жыл бұрын
Thanku ji
@sujatmanzoorbhat6616
@sujatmanzoorbhat6616 2 жыл бұрын
Which doctor to consult if hemoglobin increases in children aged 15 years۔
@paraschopra3751
@paraschopra3751 2 жыл бұрын
Thank u maam
@manojthakur7731
@manojthakur7731 9 ай бұрын
Thak u
@nandanidasondhi5696
@nandanidasondhi5696 Жыл бұрын
Mem plzz give me which article are used?
@deeznutttttttttt
@deeznutttttttttt Жыл бұрын
What do you mean ?
@zairoddinsoudagar3919
@zairoddinsoudagar3919 2 жыл бұрын
Good explanation tq
@banukumar9899
@banukumar9899 2 жыл бұрын
Super thank you so much
@ajimabegum413
@ajimabegum413 2 жыл бұрын
Thnks mem..🙏🙏🙏
@sameerraza9897
@sameerraza9897 Жыл бұрын
Maine kisi Ko protein aati ho ismein to kaise check
@ramchandrapal8430
@ramchandrapal8430 3 жыл бұрын
Hello Sir, 2021 se D Pharma ka syllabus change hua kya??
@solutionpharmacy
@solutionpharmacy 3 жыл бұрын
Yes its about to change
@safwanasafu442
@safwanasafu442 Жыл бұрын
Mam I am from kerala Please put english substile please
@solutionpharmacy
@solutionpharmacy Жыл бұрын
Video is also available in English, please check description box
@safwanasafu442
@safwanasafu442 Жыл бұрын
​@@solutionpharmacy❤
@COMPARISON--GUY
@COMPARISON--GUY 5 ай бұрын
Chup learn hindi saar
@AbhinavSwain
@AbhinavSwain 2 жыл бұрын
If the sugar is present it's colour will be orange
@Ujjwal93500
@Ujjwal93500 2 жыл бұрын
Mam, Biochemistry test practical video or notes
@ayr-i6r
@ayr-i6r 2 жыл бұрын
Mem kya analysis krke wood ki age ka pata laga sakte h 😳
@healthcarewing
@healthcarewing 2 жыл бұрын
Very nice 👌 👍 👏explanations mam
@animaoraon8665
@animaoraon8665 10 ай бұрын
Glucose solution kaha milayga
@solutionpharmacy
@solutionpharmacy 10 ай бұрын
Glucose ko hi mix kar lijiye
@sarthakkale7264
@sarthakkale7264 3 жыл бұрын
Why to heat testtube
@aaryatrivedi_1712
@aaryatrivedi_1712 3 жыл бұрын
Plz tell that..During this experiment, after heating, the smell of Urine will come ??
@solutionpharmacy
@solutionpharmacy 3 жыл бұрын
No
@pardeepdhanjal9067
@pardeepdhanjal9067 3 жыл бұрын
READ BOOK 1st dont go wrong
@kanhadiwanisujata
@kanhadiwanisujata 2 жыл бұрын
Tqu mam
@riyasharma2074
@riyasharma2074 2 жыл бұрын
Jai mata di
@clclpal5972
@clclpal5972 2 жыл бұрын
Thank you so much mam
@thekrishvikacomedy8054
@thekrishvikacomedy8054 7 ай бұрын
👍👍
@pardeepdhanjal9067
@pardeepdhanjal9067 3 жыл бұрын
did u know Principle of this test
@dipmalyapandit4755
@dipmalyapandit4755 Жыл бұрын
@samanabbas9485
@samanabbas9485 11 ай бұрын
Ji sir ham
@saijucherian9375
@saijucherian9375 2 жыл бұрын
not correct . 5 ml reagent and 8 drops of urine is correct method
@solutionpharmacy
@solutionpharmacy 2 жыл бұрын
Its not quantitative sir, its qualitative demonstration
@saijucherian9375
@saijucherian9375 2 жыл бұрын
@@solutionpharmacy okk
@riyaskpmkdriyaskpmkd210
@riyaskpmkdriyaskpmkd210 2 жыл бұрын
Malayalathil viduka
@salimrazal7103
@salimrazal7103 11 ай бұрын
Kon 2024 exam ke liye dekh raha 😂
@Vivek-ey1zr
@Vivek-ey1zr Жыл бұрын
But it's looking like orange color 😢
@solutionpharmacy
@solutionpharmacy Жыл бұрын
In camera colour may be variable
@sapnasomyasomya2204
@sapnasomyasomya2204 2 жыл бұрын
Ch bsc zoology
@manojthakur7731
@manojthakur7731 9 ай бұрын
Thank you ❤
@katy34239
@katy34239 Жыл бұрын
thank u maam
@chinnaswamyswamy4161
@chinnaswamyswamy4161 Жыл бұрын
Thanks mam
@sheshnarayansahu9793
@sheshnarayansahu9793 2 жыл бұрын
Thanks di
@Atulmaurya742
@Atulmaurya742 2 жыл бұрын
Thank you so much mam
@SarithaUnni-ye4ps
@SarithaUnni-ye4ps Жыл бұрын
👍👍
@AnilSingh-pc7qt
@AnilSingh-pc7qt 7 ай бұрын
Thank you 🙏
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