Bioinformatics for RNAseq

A recording of a live Zoom training for Bioinformatics for RNA Sequencing Analysis from the Tufts Data Lab, with Wenwen Hou, Rebecca Batorsky, and Albert Tai.
Recorded in May 2020.

[@Knoyka6665]

Rebecca's explanation is clear about normalization without accounting for gene length. Thanks!

[@kiplimosimon1429]

Excellent work. Much appreciated

[@MrRamaeri]

thank you very much, it is very helpful to me

[@linhlibuit2232]

Nice!

[@Checray]

Is the dna seq workshop mentioned in this video available to watch as well? I'd love to watch that too!

[@myzzz01]

Unfortunately the DNA seq workshop was not recorded and hence not available on youtube. But please check out the self-guided tutorial here: rbatorsky.github.io/intro-to-ngs-bioinformatics/

[@samuelmensahbaffoe8303]

how is the condition assigned to the design before using dds

[@zhanglisa4548]

I am going to perform a pilot experiment with two samples from the WT group and two samples from the Null group. Are there other samples I need to send for RNA sequencing? I saw other people also included controls for sequencing. I don’t know what I can use as controls.

[@pedriclaassens3145]

Heads up, with only 2 samples you won't be able to do stats on your RNAseq and it will tell you very little. It will be a waste of money.

[@pedriclaassens3145]

Is there a way for me to get access to the DESeq2 and Functional Enrichment code? I have finished my Differential expression using the old and trusty Tux pipeline. But the post differential expression data presentation I am struggling with.

[@myzzz01]

Here is the github page this video used: huoww07.github.io/Bioinformatics-for-RNA-Seq/ More information can be found here: hbctraining.github.io/DGE_workshop_salmon_online/schedule/links-to-lessons.html

[@mariacarolinaescalonaweldt7446]

Hi! Where can I find the script to process the reads ??