Primer design |in silico cloning | SnapGene | UCSC Genome browser

[@gutsandglory7507]

Guy you have nailed it. Thank you so much for this wonderful video. Straightforward and clear crystal. Greetings from Russia

[@BiologyLectures]

You are very welcome

[@gutsandglory7507]

Thanks once more, I tried all the steps (with 3-4 deviations) using my assignment project. It was a success. I am glad to find this tutorial on my time of need.

[@BiologyLectures]

Very glad to hear that this video helped with your assignment. Please like and share and subscribe to our channel to support us.

[@jacobbkgaard6735]

15:09 if I am not mistaken, the '8, 10, 12, and 14' in the table do not refer to digestion time in hours but rather to the number of nucleotides at the cleavage site. However, the two rightmost columns refer to %-cleavage after 2 hours and 20 hours. This information is quite critical - especially for optimization.

[@muskansyed2037]

Just the thing I needed to observe carefully before kickstarting on my first cloning experiment. Wonderfully described aspects of cloning by you Sir. Hats off

[@BiologyLectures]

Thank you very much for the kind words. Please like and share the video and subscribe us to support us.

[@ericwuluglayjr6060]

Thanks also teacher, God blessed you 🍒

[@BiologyLectures]

Thank you very much. God bless you too

[@SparkBaby-o6p]

very useful just saw this before my practical ... very well explained

[@BiologyLectures]

Glad you liked it

[@AmruMagdy]

Thanks also teacher, God blessed you

[@BiologyLectures]

You are most welcome

[@negessemekonnen1854]

very important demonstration, thanks!

[@BiologyLectures]

You are most welcome 🤗

[@mishralab2221]

Fantastic job explaining this! Thank you.

[@BiologyLectures]

Thank you very much.

[@shrilaxmibhat9713]

Thank you for the video.Excellent explanation given, 😊wish to see more such videos in future.

[@BiologyLectures]

So nice of you. You are most welcome. Please subscribe us to support us.

[@muhammadichsan2558]

Very nice explanation.. Keep going

[@BiologyLectures]

Thank you very much:-)

[@magdaescobar3697]

Excellent video. You helped me a lot with your explanation

[@BiologyLectures]

Glad it helped! Please subscribe our channel to support us.

[@dikshyapanthi7681]

Excellent explanation I was looking for something like this

[@BiologyLectures]

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[@SanjaySingh-oe1ts]

Good Explaination. thanks

[@BiologyLectures]

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[@Hari788]

Excellent work. Thanks .

[@BiologyLectures]

Thank you very much. Please consider subscribing us for more contents like this.

[@sadullaabdullaev5451]

Very useful and excellent work, it would be great if you demonstrate how to use Ensembl plants !!!! thank!

[@BiologyLectures]

Thank you, we will certainly make a video on Ensembl plants.

[@ogunoluwamayowa4749]

Amazing explanation. Can I also just design cloning targeting just one exon?. I mean like you just did for 3' UTR. Just coping the targeted exon sequence

[@BiologyLectures]

You are most welcome. Yes you can use just one exon or any other part of the gene to do cloning.

[@ogunoluwamayowa4749]

@@BiologyLectures Thank you. I subscribed to your channel

[@BiologyLectures]

@@ogunoluwamayowa4749 You are most welcome and thank you very much for the subscription. Please share the video also.

[@sreeram6416]

Sir I'm given a task of cloning the cDNA sequences of all the rna binding proteins in a vector called pEGFPN-3X-HA, which contains the HA tag. I retrieved the cDNA sequences of all the RBP's using Linux commands in a single go, and now I want to clone the genes into the pEGFPN-3X-HA such that these RBP after they are cloned and expressed will have HA tag in it and I can pull it using anti-HA antibody. My doubt is could you suggest me any software or tool in which we can synthesize primers in one go for these RBP such that they have respective restriction sites in them and they express the HA tag also

[@deejaywonderer9814]

where can i download the software you are using here?

[@BiologyLectures]

PLEASE FIND THE LINK HERE www.snapgene.com/vector-nti?gclid=CjwKCAjwr56IBhAvEiwA1fuqGgewE9pWgM2Gns0Z0rB48r4wg8U882APQYTZ9yVCjNjaW2epxaDYhhoCDa4QAvD_BwE