CRISPR System and CRISPR CAS9 Technique, The full principle (Part 1)

[@priyamvadam4928]

really good. much more detailed than most videos available on the same topic.thanks.

[@lembuhota]

True

[@anushabs6599]

Exactly!!

[@chemistrymattersgetit4161]

Very well done, great for both undergraduates and the more advanced. I found especially useful the way you distinguished between the 3 different systems, which is often not clear to those who are first learning about the system.

[@merttantari5473]

finally someone who explains this topic very clear and also detailed enough :) thank you , it was really helpful !!

[@maximilianfischer9198]

Great video, just a minor suggestion: I would give a short general overview (basically the summary that you give at the end of the video) of what the system does and the goal at the beginning of the video and then go in detail.

[@NookKana]

Thank you so much. This is so helpful. Great video and great explanation :) I can understand about CRISPR system better in just twenty minutes. Thank you

[@biomedicalandbiologicalsci4989]

Thank you for your comment :)

[@70194tanner]

At 8:12 you refer to the lower strand as the coding strand. I believe that this strand is actually the template strand because the newly synthesized strand would have the same sequence as the top strand. That would make the top strand the coding strand and the bottom strand the template strand.

[@mohinitalwar2888]

You are right the coding strand is actually the Non template strand as it contains the SAME nucleotides as that of mRNA while the template strand contains the COMPLEMENTARY nucleotides

[@fagusformigordusfagordumfl1798]

yeah right

[@LKVPAWAR_ycmou]

Exactly right.. bottom strand is template ..

[@LKVPAWAR_ycmou]

@@mohinitalwar2888 I didn't got the Non template strand concept please explain what u said in didn't understand it please

[@mohinitalwar2888]

@@LKVPAWAR_ycmou as you know there are two strands, template and non template. And because template strand has the complimentary sequences with that of mRNA (the complementary rule) that means the another DNA strand (non template strand)has the same sequence as that of the mRNA (except T in place of U) that's it is called as conding sequence as it has THE SAME CODES as that of mRNA.

[@arkveth6921]

I believe that at 10:43 you wanted to say Cas6 homolog (as written in diagram) instead of Cas9.

[@lukengai4956]

tysm this was what i was looking for for so long ;-;

[@lucasalopez-jirau4714]

Thank you!!! Excellent for undergrads having trouble with the primary sources; it really helped me. * thumbs up *

[@amrutvani2]

If a bacterium can cut off piece of viral dna in the first encounter, there is no chance of it (viral dna) integrating the bacterial dna. So why this complicated mechanism is needed ?

[@chuti_vanila]

Thanks​ you​ so much, you​ explained clearly for​ this​ topic.​

[@IbuWdesta]

Really nice presentation, much more detailed. Wait for your next presentations

[@desuranus7349]

At 9:15, cas6e/cas6f cut at the junction of ssRNA and dsRNA. The graph is not correct. It should stop at the end of hairpin loop. But this is minor detail. Many thanks for the videos. This is the only CRISPR video in English I can find on youtube that teaches type I, II, and III. Thanks!

[@lethaltrix316]

GOAT CRISPR Video

[@garyraab9132]

Informative content, with simple and useful animation. Having produced hundreds of science videos and animations, some simple suggestions for the sound track. First, write out a clear concise script. Do a second edit on the script. When recording the script, speak each line clearly, leaving a long pause in between each comment. Avoid ummms and awes! With audio editing software it’s easy to adjust the length of the audio to match the animation. With the audio editing software, it’s easy to recognize and find, ummms and awe waveforms and replace audio distractions with silence.

[@mirzamudassir3937]

Thank you so much for this vedio I exactly understand how crispr work

[@waqarhussainmian8748]

I like this vdeo.but i have question. Diff viruses always attacking due to crispr bacteria add virus genome snnipts .Does it harm bacterial Dna bcoz it is put in spacer Dna and how long will bacteria keep it in spacer Dna bcoz new viruses attacks bacteria i think bacterial genome will no space if many viruses attck for long time.plz clarify.

[@RyonBang]

at first i thought it was Ariana Grande's voice teaching CRISPR and am like "whut"

[@kathleenranihagen9256]

This was great! Now I finally understand. Thank you!

[@biomedicalandbiologicalsci4989]

you are welcome :)

[@md.ridwanahmed1769]

Do you have this crispr 2 video as an article???

[@edthoreum7625]

4:09,3 steps 1-spacer acquisition,4:21 2-crRNA processing,7:30 3-interference,10:55

[@Shadowdarknessone]

a really great video. I just dont understand one thing. where did you know where to cut. And how did crispr knew where its needs to cut and replace the dna/rna or modifie the dna/rna. As far as i understand it crispr splits the rna/dna up but where did know what you need to pick.

[@qaisarshakoor09]

Thank you very much for the explaination

[@OrlandoRodriguezHK]

Many thanks for the video, it is very clear. Well done.

[@OzgeSonmezler]

Hi! Thx for the video, helps a lot. Is there any place i could find it as pdf or ppt?

[@mohammedhasanalmashat846]

Thank you ,it is very useful video . I have Q if virus invade a bacterial cell ,it will insert genome and use bacterial cell to produce compunents to produce new phage and finally, bacterial cell will lysis . the question is how can bacterial cell save piece of viral genome when it will be degredate after viral invading .

[@thurayaelbanna5773]

Sometimes the bacteria resist the phage so it can take a part of its genome, and pass this genome to the next generations.

[@ryanhimawanwibowo6014]

Thank you so much!! well explained :)

[@wdyafw]

This video explanation is very helpful especially for a beginner like me. Could you please also explain how CRISPR/Cas9 disappear from the genome of an organism? Thanks.

[@sumantamohapatra6727]

Literally very informative mam

[@lucho0849]

very good explanation am able to better understand the processes involved

[@stephenprice3357]

seems like it would of been a nice video if the closed captioning was fixed

[@BeUWinteer]

Thank for sharing this video. It very greatful.

[@anshumathur7585]

Thanku mam to share this video Its good enough to understand the mechanism of crispr bt i hv a query regaring this as u told about crispr mechanism to bacteria protect from viral infection as immunity, bt there is also the lytic or lysogeny cycle where they protect from adverse conditions what about ds?

[@farhanabbas2728]

Very helpful video about CRISPR.... Thanks alot for guiding

[@jagannathswain5288]

WHERE TRACR RNA FORMED? HOW USE IN GENOME EDITING?

[@eddiewampande5107]

This was fantastic, i can now apply the technique of crispr in my research

[@PJ2668]

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[@mdshahidulislam6805]

Yes, it was very good to understand easily. Thanx.

[@DhobyVic]

Thank you for a very helpful explanation. It's not an easy topic and I will need to watch the video again a few times for it all to sink in but, essentially, and for the first time, I feel I do understand.

[@aws6097]

why bacteria need crispr if it can cut the dna of the phage in the first place

[@Raul28153]

The Cas9 protein has six domains, REC I, REC II, Bridge Helix, PAM Interacting, HNH and RuvC. Why did you say it has two? Is that because you are only concerned with the two (HAN and RuvC) for the purposes of this video? Or are those two the only ones used in the current state of CRISPR technology?

[@aaryashashi173]

This is one of the best detailed video on CRISPR i have seen in KZbin

[@IshitaJain10]

A great help...thanks

[@questforenlightenment441]

I have to do a presentation about the CRISPR/Cas System and couldn't find any conclusive, highschooler-friendly texts. Thank you so much.

[@CropScienceyt]

Detail information in simple language with presentation

[@giuliarossetti197]

Thank you very much! It saved my lifeeeee! :D

[@medineadin4700]

Thanks, it is a very good explanation

[@nancyanderson5413]

Gosh I saw a really good video before that was so clear, she really pooped my understanding here. She explains really well lab procedures but she sucks explaining biomolecular concepts

[@rikkigupta9546]

thanks alot for easy explanations could u pls send me its refrences

[@animeshhb]

so nicely explained ! A big thanyou for making me understand the whole process in a easy way !

[@unays]

can anyone tell me, effects and characters of short and long PAM?

[@railroadmovie]

Whats the difference between the three types of interference described here? Do different species of bacteria use different types of interference or do these three all occur in the same cell simultaniously?

[@joelrutta3658]

what is the difference between CRISPR and RESTRICTION ENZYME

[@ankushbajaj5764]

👏👏👏GREAT JOB. pls keep uploading videos on ur channel. 🤗🤞

[@adriennegallotta2890]

Yes when this mortal puts on immortality And this corruptible puts on The incorruptible Then comes the end If you have not heard God made the heaven and Earth and everything in it

[@markhajdari8591]

Hay i have a realy important question where and what would one need to buy in a super market or plant to explore ones undersanding so can i buy any enzymes in store to explorer ones own health

[@urvashibhatara3626]

Very nice video

[@christianadipratama2310]

Does it always be 5' to 3' that became the template DNA? I was taught that 3' to 5' is the one that will undergoing transcription.

[@maomaotan8270]

YOU ARE AMAZING!

[@dimitragennimata89]

Excellent presentation. Ideal as an educational resource, for explaining how the system works in bacteria. Very well done, indeed.

[@biomedicalandbiologicalsci4989]

Thank you for the nice review ... stay tuned :)

[@AnkitKumar-nh5io]

Biomedical and Biological Sciences very good explanation

[@kofipapa2886]

​@@biomedicalandbiologicalsci4989 The nice reviews are nice but the not so nice reviews are also very necessary for growth and learning your self because you don't know it all. I love your video and the motivation to help people understand. I have learned by you. Thank you. But! It still remains that the mRNA transcripts isn't complementary to the coding strand( 5'-3'). It is always complementary to the 3'-5' strand ( anti sense/ template strand). And this should be viewed as nothing but a closed loop feedback. Nice reviews feel nice but the feeling aside it does nothing by way of promoting learning as ' not-so-nice' type of reviews do. Thanks for sharing your knowledge. I enjoyed your class very much.

[@zph1806]

Thank you so much for your very nice explanation it wa really very helpful ❤️

[@sarmadElhassan]

Very helpful, however PAM position is on the non target strand 3’ to the protospacer

[@v.ariabod5488]

excellent and very useful video and explanation

[@suvethas1851]

Very well explained 👌

[@barbarvssa]

Coding strand is not the one transcribed 8:16, it should be the template/non coding/antisense/transcribed

[@RealJulWhite]

Great Video! Have suggested the channel to my professor so he can recommend throughout the next years

[@woodpecker6452]

Why does the bacteria need the crisper system if it can neutralize phage with first infection

[@ximenaze8242]

Great video! Thanks for sharing

[@ahmedmohamed-iu2fj]

Wow I’ve watched this couple times and u explained it very well. Thank you

[@danielholley2495]

Excellent video. Thank you so much!

[@Xbox360MatureGames]

pls explain this in russian its hard to follow with all the stuttering

[@zahidkhan5532]

Biotechnology without microbiology would be impossible.

[@bdhome2010]

thank you. it was an awesome video.

[@martingitonga5436]

Wow, very help basics! Thank you

[@bharathreddy9337]

thank y very much and I like your video presentations. have y made any videos jumping genes(transposons)?

[@biomedicalandbiologicalsci4989]

Thank you for your comment, I will try to do it :)

[@andreagoh6198]

thank u for teaching me what my teacher did not teach but wants to test T.T

[@aadem7744]

could you provide the Sources for me?

[@monakhazaei3324]

What is the difference between gRNA and tracrRNA?

[@arvindgond4149]

Very well explain I think 1st (this video) is essential to create base to understand CRISPR . Thank you for breakdown it and explain it in the simplest way 🙂

[@utsavvaghasiya]

very detailed and easy to understand this complicated mess. thank you

[@srishtisharma3200]

Amazing video !! You win it !!!

[@ahmedalfahad3866]

Thanks very useful

[@majdr.sweidan4606]

Hello thank you for the interesting vedio, i really enjoyed it, but I have one question, Im smolecular biology and genetics student and I have studied that the coding strand is the strand that is identical to mRNA " thymine instead of uracil" but in the vedio you said the the coding strand is the strand that was transcribed into mRNA , can you explain this to me ? Thank you

[@kianakhz4558]

Very helpful.Thank you

[@tukombemuleya9689]

well well. this was so clear ma'am. i understand it well

[@nancyanderson5413]

Her other videos were nice, this one is not good at all

[@lokeshgowda9513]

Jhhhnn

[@lembuhota]

So simply explained with details... And also very simple and nice animation.... Great work... Keep explaining topics like this.... Your video helped me a lot... Thank you 😊😊👏👏

[@hemastandard7649]

Great video I understood really nicely and loved the accent, very understandable (perhaps you can make an updated video on the subject).

[@dennisalego6548]

Thanks so much, exactly what I have been looking for 🙏

[@Theasan3]

Why start 3' and end5'? And 5' end 3'?

[@mainebehavioralhealthcare669]

I have the captions on and everytime cas is said it comes up as a different word, cats, caste, gas, class, caps,case, castor, lol everytime

[@dikshashreedevi4568]

very usefull...plz make more videos.

[@VijaySingh-tg1be]

So far the best video on CRISPR basics. Easy to understand in layman's langauge...

[@_MercyStephen_]

You made it so well

[@Wathiq199317]

thank you it was very clear and easy

[@rihsabumtahb9801]

Do Eukaryotic Genomes Also Contain PAM sequences Like That Of Bacteria(Prokaryote)????

[@itsmepiyu1227]

No it is not present but we cam add by cloning vactors etc may b ...

[@nika_cm]

thank you, this was very clear and easy to understand, it helped me out a lot!

[@saintamaria5598]

Please i have question . How to explain having diffrent size of deletion by using the same two gRNA

[@debashreedas9897]

Well explained. It helped me a lot! Thank you so much!

[@harunkimathi8515]

thank you! very clear