Quite helpful. I was confused on the part of screening but i think this video puts it together nicely. So, it's a conjugated enzyme that finally binds to the secondary antibody. On binding, the enzyme converts the colourless substrate attached to it into a colourful insoluble product that sinks down to the nitrocellulose membrane emiting colour. Got it! 💪
@antoniomele4365 жыл бұрын
This was very helpful! Thank you!
@CatalystUniversity5 жыл бұрын
Glad you liked it!
@noha18784 жыл бұрын
Great video. Thank you.
@saharzaki23104 ай бұрын
But what is the point of the blocking step if the antibody would never bind to anything else?
@叶之-p8z17 күн бұрын
because you wouldn't see it on the membrance, colourless. And the purpose of this one experiment is to purify the protein, meaning get it out of all the crowd. When the secondary antibody binds to tthe soluble substrates and form those insoluble products, it will stick on to the memberane, and only them are the ones left.