How to Make Liquid Culture | Liquid Culture Lids | Take a Live Culture | Inoculate a Spawn Bag

[@donivanhummel3106]

Thank you, as a person who is new to mushroom cultivation, I appreciate how step by step you are with your videos, you're an excellent and thorough teacher.

[@BoomerShroomer]

thank you!

[@jesush.christ5978]

You don't need an injection port. put two layers of foil on the top of your jar. Lift up the first layer and inject culture or spore into the jar. It's been working fine for me for years.

[@trump45and2zig-zags]

Any other tips for anewbie? Spores n all in 1 grow bag should be here this week

[@jesush.christ5978]

@@trump45and2zig-zags make a still air box. Its hard to explain, you get a tote and make two holes for your hand and get some pvc pipe or something your hand can fit through. Glue some of those big rubber cleaning glove to the inside and seal everything up. I put glade wrap over the top of mine so I can see better. Put everything you need in, and spray some rubbing alcohol in, then put the wrap over it and let it sit a while.

[@trump45and2zig-zags]

@@jesush.christ5978 like a sandblaster, I gotcha, thanks. Excited to grow my first batch when I get everything this week

[@trump45and2zig-zags]

N you pretty much just make a foil pouch with the jar, right?

[@jesush.christ5978]

@@trump45and2zig-zags are you using jars and doing the brown rice flour tek?

[@joey_btw4718]

Wow! Thank you! This was by far the most thorough step by step video I've found so far, and I've watched probably a dozen or more

[@zoghunter82318]

Pro tip for anyone doing this for the first time, always run your drill in reverse when drilling plastics to prevent cracking.

[@zoghunter82318]

@Valerian Roots Yup, if you're drilling plastic, no matter the size of the hole, run the drill in reverse and don't push to hard. Works every time.

[@Boosacnoodle91]

My guy you are my hero. I’m starting up my company and trying to get all aspects for sale, including culture lids. Went to mass produce them and about had a stroke from how frustrated I was getting from the lids cracking. You are a god.

[@FLORIDAMANIAM-he2lz]

Pro tip. Use a one of those triangle stone bits and drill through like you would a step bit. Its just sand through so there is no risk of craking or breaking at all

[@madmaxofspokane1691]

Or use a drill & brad point bits...

[@fun_ghoul]

I don't understand this comment. With no special effort, I've never cracked a plastic mason jar by drilling. Frankly, if I was doing a shotload (ahem) of these, I'd probably pop for a drill press and do 10 at a time.

[@jasonnascimento9174]

I never comment but I want to thank you for the way you instruct. There are many out there but I keep coming back to your info because it is basic in the best way possible. Sometimes it gets so confusing. Your instructions are very linear. Thanks.

[@doinksinthePM]

10:45 it's also the perfect time to 'noc up a few more jars with that LC so you can save some time on turnaround. Instead of having to go from the tissue biopsy stage next time. Lovely video BTW! Makes me want to get growing again. So much faster than cannabis with zero electricity required and basically one brief moment of light just to start pinning? You can't beat that. I'm just super weary about trying to go myco-psycho again because the last go around I got arrested for unrelated reasons and wasn't able to return home for a few weeks. By that time I had 8 Rubbermaid tubs that were just an orgiastic death feast of aspergillus and black mold plus some contams I'd never even seen before. Ever since then I can't even keep brand new bread in this freaking apartment for 2 days without it molding. I'm terrified if I put new tubs in they'd have the same exact fate.

[@rartucio]

By doing the tissue biopsy you select the best genetics from the mycelium. A pity to hear what happend. Anyways if you esterilize properly Im sure your not going to have any problem.

[@semmett22]

Where do you store your liquid culture jars, A cool dark place, a Fridge…? Also how long will a jar last before it’s no longer good to use? Btw, Thanks Ashley for another perfectly narrated video!!🫶🏼

[@WhiteWolf1980]

Once growth slows down in the jar, you can store it in the fridge for up to 4 months.

[@WhiteBoyWayyysted]

I've made mine and put it straight in the fridge can I take it out and let it grow a bit and put it back?

[@rattlesnakenate8732]

This is probably the best instructional video I've ever seen!!!! Thank you!!

[@jameskreiter]

Ashley, you are AWESOME!!! I have asked you two questions, and you have answered them both. Unlike Cameron the Grain Man where I bought some grain, he did not answer me on YT or via email. And guess what, he sold me bad grain, did not spawn a bloody thing. So big huge thanks to you, what your are sharing, and doing. What you are sharing is invaluable to all of us Mycology Students.

[@BoomerShroomer]

Thank you. Did Cameron replace your bags? How many were bad?

[@jameskreiter]

@@BoomerShroomer I ordered five, the first was bad. I lost one bag, and the spores. I have four left, and there is no way I will try them. That is $50 down the tubes.

[@BoomerShroomer]

@@jameskreiter how do the other ones look? As long as they smell good and you can’t see contamination they should be good to use. I use his bags lot and rarely have issues.

[@BoomerShroomer]

@@jameskreiter I’d be happy to refund you your order from them.

[@jameskreiter]

@@BoomerShroomer They all look the same. and smelled just fine. I am afraid to even try them. Moving forward I am doing the whole process in house. At least I only have me to blame.

[@stevenscrafting2467]

If you forgot the aluminum foil also I found a really good way of keeping the coffee filters over the jars by using the original ball rim the The jar came with Nice deep and snug

[@guymontag2948]

I bought the HaBeuniver 20mm injection ports, the shorter grey ones that seem to be very common, and they'll fall right out of a 1/2" hole but a 7/16" is a perfect fit. Thought this might save someone buying the wrong bit. A brad point bit works great on the standard metal lids, btw. Great video, Ashley, as always.

[@sonotuttotuo7782]

Thank you immensely. I like your video. As a beginner. I have to watch it over and over again.

[@addisondraper3574]

The best video on the subject that I've seen. Thank you.

[@BoomerShroomer]

Wow, thank you! I appreciate it. 🍄❤️🍄

[@Gnarmarmilla]

Hey Ashley, check this out, this is from a fellow named Marc R. Keith who has been growing since the 70’s, “Throwing spores into honey or karo has to be the worst possible way to make a liquid culture and I'll be glad when this current fad passes and folks can get back to established techniques. Just inoculate some grains or brf in the standard way and you'll be miles ahead. For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores. Upon full colonization, shake the jar to loosen each individual kernel. Be sure to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc. In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains. 3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out. You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes. Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this. Remember, mycelium grows poorly in water unless under constant stir which oxygenates it. With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels. LIQUID CULTURE - A glovebox is not advanced equipment. One can be made free with stuff around the house, so there's no need for people to work in open air or fecal and mold infested bathrooms and kitchens. There is also no way to generate 100 full syringes of liquid mycelium in very small pieces within two weeks from injecting spores into honey or karo. I'm sure if people blindly used the grain method I described above, many would still have problems too, because they're inserting unproved and possibly contaminated spore solution into grains, which also isn't proper mycological technique. However, agar can easily be poured in a glovebox, and the Petri dishes used to isolate healthy mushroom mycelium away from any contaminants in less time than it takes to even know if a karo LC is contaminated or not. That agar wedge can then be used to inoculate the quart(or pint) jar of grains, that can then be turned into at least 100 syringes, or used for g2g transfer to get ten jars the old standard way. In addition, after the water is poured out of the grain jar, it can be placed back on the shelf to re-colonize, and the process can be repeated again. I just strongly feel new growers are doing themselves a disservice when they simply inject spores into honey water and then sit there waiting for something to happen. At the very least, inject directly to grains so you can visually inspect the process. Perhaps Agar's lids with injection ports will help those who have never used grains to get off to a good start.” From, “All of RR’s notes.”

[@Filmafamily93]

Hello. Excuse me can you make champignon mushroom seeds ?? i want to learn to make it

[@ericweaver1693]

Thanks!

[@Henninchs]

It's very proper but without the equipment it can work as well. I can use 5% agave syrup into sterilised water in a jar with a hole filled with silicon. Using small sterilised atones or glas marble in the jar to shake it 2 more times a week. Anyway, it's probably not as good as with this equipment but I can tell it works as well. If you grow a lot you probably improve your equipment anyway to minimise your failures and experience.

[@joepangit6938]

Amazon has those lids in colors, with silicone gaskets. They are sweet. I use 1/4 drill grey injection ports, and 1/2" stick on air exchange glued down with red high temp RTV sealer. They work great!

[@ohiosu55]

What’s the lids called?

[@ShootYourRadio]

Is that an injection Port filter? I have a question about injection ports when you tip the jar over doesn't the filter get wet?

[@karlkramer7687]

Any tips for making a flow hood? Where do you get supplies? Thanks!

[@poppyjones1892]

Very nice personality , something feels relaxing and refreshing they way she teaches 😊

[@naftalibendavid]

Always grateful for the info as well as your optimistic attitude

[@BoomerShroomer]

Thank you for your kind words! :-)

[@panda-gy4ni]

What size needle is that?

[@masacatior]

Knowing you can grow LC from biopsy instead of spores helped me a lot! Thank you.

[@heatherrichart-bishop5412]

This is really good sister. I like all the comments as well. Truly- I've been looking for the right videos.

[@BoomerShroomer]

I'm so glad! Thank you!

[@Chris-dy5fy]

Liquid culture is definitely the way to go.

[@rosssparks197]

What a greatly informative video. I would love to enjoy one or two of your fungi and then kindly request that you narrate my experience with your wonderfully soothing airline stewardess voice. So very calming. ❤

[@Bootysmoothie]

I love the way you hold the jar to get good angles on the pieces, you can see the growth off tot the side of the first little lump of mush

[@hazarkasem353]

The most beautiful channel ever I have a question 1 what kind of mashroom is this 2 is this one for magic mushroom ?

[@myco_chaotix]

👀👀👀👀👀

[@nexusnostalgia]

Love that you use unbleached filters!

[@BoomerShroomer]

Thank you!

[@tokkichainsaw2371]

this is the best tutorial so far! thank you so much!!!

[@kerrymitchell3982]

What do you do if you are using a spore print instead ?

[@krazylife224]

Same technique except just scrape the spores into the jar under a SAB or in front of flow hood, sit back and watch it grow

[@orionsbelt3827]

Hi Ashley I have a newbie question. What temp do you leave your liquid culture for colonization and is it in complete darkness the whole time? And when the jar is fully colonized, how long can it be stored for?

[@johanhallgren]

@@nailinyourmom I thought you should store it in the dark. Is this 12 hour light cycle really necessary. I have not tried LC myself yet but will soon, so am really interested in the optimum conditions.

[@robinhoode9364]

Very informative and well-made video. Top Quality! Thank you.

[@jeffgood6441]

Hey what kind of mushrooms where those they where absolute monster good genes for sure

[@heywoodjahblomie325]

Hey Ashley what gauge needle do you use to extract the LC then of course, inoculate the fresh grain jar please? I see blunt and Sharpe needles Thanks.

[@rogueleo6587]

Is it possible to get spores and create a culture from dried out dehydrated mushrooms?

[@myco_chaotix]

Yes, but the germination and/or growth potential of your sample will be quite low. Mycowilly posted a video recent on YT that walks through bringing dehydrated fruit “back”

[@chandracox6814]

Can I store the LC mason jars in the fridge?

[@judy1879]

Good question 🤔

[@juneconnor8127]

Where did you store your jars?? Do you incubate at a certain temperature ?? Do you put it in the dark/ Thanks so kindly for any information !! Wonderful Video... :)

[@homewreckerrob]

Where do you get those long needles to suck out the lc from jar?

[@naturallydope247]

Great video! Loved it. This was one of the better explained ones.

[@BoomerShroomer]

Glad it was helpful!

[@alum798]

hi , really like your videos just wondering how and how long can you store your bottle of liquid culture

[@user-qm4qb6le5v]

So wat if I don’t have a muchroom to get it out of so I can grow wear can I get it from

[@mattborski]

Chicken or the egg situation. I hear you on that!

[@JedF2010]

It's great that you put links to everything used. Also nice to see info on your camera kit. Great vid!

[@BoomerShroomer]

Thank you!

[@soccer2themax]

Love your technique and explaining style. Very good

[@BoomerShroomer]

Thank you so much!

[@ducpham2007]

Even your voice, so good videos lady !keep them up !

[@MrEiht]

Thank you so much for the footage of the development. I put Lion's mane LC in such a jar to let it grow. After a week I only see a tiny bit. But I asked smart people and they said it is alive. But way slower than the life you showed there. Good luck with your work!!!

[@hoseaahadi4889]

Hi madam Ashley,thanks for your video I have lent especially the way you find messenger and then innoculate to dishes

[@sherrypayton6871]

I have a lot of questions about this actually. I haven’t done it before. I’d like to know about the benefit of doing it this way versus just getting a sports orange because it seems like a lot of expense and cost to do it this way and as far as how long it takes, I don’t think you’re saving any time in the long run. Also, how do you store that life culture and how long does it keep? But of course, I love your videos. They are my favorites and I always watch yours when I need to learn something new.

[@DevoutJourney]

I’m brand new to this. Built a still air box, I have a colonized spawn bag coming. I’ll be making my own substrate. I have everything for agar plates, slants, and am wondering would it be asinine to also have LC? I’m growing lions mane as my first go.

[@jacobcudmore-maupai4963]

How do you keep the culture alive during storage? Do you need to feed it?

[@TutorialesdeNaraCarolina]

Gracias Ashley. Muy clara y didáctica tu explicación. Me diste mucha información útil!

[@603VIL]

Where do you store the unused portion of the liquid culture? How long is its shelf life once its "finished". Also, when making it, where do you store the jar when it's colonizing over the course of those days?

[@kellyroup4262]

Are these injection and gas release ports reusable with sterilization and where do I get them. Could probably find them but thought you might be able to let me know.

[@joshuatucker7939]

I was wondering can you run this unit without the optional stand? Thank you for your time

[@danielreyes6646]

How do you recommend storing the liquid culture or the agar dish if we wish to keep it? Thank you!

[@colliermorris]

refrigerate it

[@richmondnuamah5440]

Please with this method can I use honny Instead of agar powder?

[@Trenscendent]

Another fantastic video! Very well presented and easy to learn from. I will definitely be giving this a go in the near future.

[@BoomerShroomer]

Awesome, thank you!

[@chuckcharlebois4199]

Ashley. You’re the best. Love your channel. What gauge and length of syringes are you using. Mine seem small and these look best for liquid culture

[@Trenscendent]

@@chuckcharlebois4199 use draw up needles

[@davidmireles9774]

do you leave the stir stick in the jar before pressure cooking it? or take it out?

[@davidmireles9774]

6 months later I rewatched this and had the same question and had a good laugh noticing my previous comment was exactly what I wanted to ask again. Any chance someone can answer this question on the main thread. Thanks! 🎉 great channel

[@MerrillBrown]

Hi Ashley great video I’m about to do my first LC project. Please tell me where you got your syringe and looks like a biopsy needle. Thank you

[@rexiwhit3616]

Does it matter what kind of grain? And also how would I sterilize the grain without having to buy special bag from online

[@ClownWhisper]

Here's a question what gauge and length are those needles where do you get those I've never seen those super long needles I've only ever used regular ones or long blunt ones but if you're going through a self healing port it's best to have something sharp or you're not getting through it

[@CharlieRocRevolution]

Are you heating it as it mixes? Thanks

[@ClownWhisper]

Igrow mushrooms I've been doing it for about 10 years I would say I have no problem with infections ever on Agar. But it's a lot easier to get infections in liquid media for some reason I don't know why it just seems to be a lot easier I mean I have had probably 5050 infection to success ratio for liquid cultures that which is terrible.

[@samurachi420]

The air port things. What do they do and how does it work? Does it allow oxygen into the jar? Or does it only allow co2 to expell?

[@fun_ghoul]

The plastic lids do *_not_* seal against the jar. This may not be an issue for this process, but it's generally good for anyone using mason jars to be aware of this fact. Also, for people without a magnetic stirrer: An Oster blender (a.k.a. "Osterizer") blade assembly and sealing ring will fit a wide-mouth mason jar! Cheers from elsewhere on occupied Anishinaabe land.

[@Bonecarpenter.]

Do you tighten jar lids or leave them loose in pressure cooker? Also do you leave liquid culture at room temperature after inoculating until sufficient growth?

[@arce102]

Where do you get the long syringe needles?

[@justicemccann7916]

I have a question! If your pressure cooker only reaches 12 psi will that be good enough for LC? I've heard it's good enough for brf jars

[@cg0401]

Thank you so much for sharing your knowledge. 🙌

[@mr_hart777]

Can I use some left over liquid spores to inoculate the liquid spore culture? Great videos! Keep em coming!

[@domiversaix5375]

This is my question! Did you find an answer?? Thx!

[@pacificcoasthiking]

Liquid culture is not spores it is live mycelium Yes a syringe of culture will work but spores need to germinate into mycelium so it has to go to agar/mycelium/ then to a LC She said this a few comments prior 👍

[@Akasha_Silver]

Hi,where do you get those extra long needles? What gauge are they? Thanks

[@alazymexican5377]

Hey Ashley I have been watching your videos for the past months and I’m leaning towards growing some myself.. I was wondering if you can ship me some spore prints so I can try growing my own.. I have my agar ready and everything I just can’t seem to find any sites that sell spores:(

[@DanielTorres-rw2rz]

Did you start growing yet?

[@almcm8120]

Thanks for your Time Ashley. I am using Raw Honey from a local Bee Farmer, to do LC ..Great Video, and Thanks again for your Help with the Hepa Filter Math!..BtW Did I Mention Theres a Fungus umung us?! Lol! Have a Great Weekend Ashley ! PEACE!

[@BoomerShroomer]

Thank you!

[@jasonpelton7252]

R u using 4% honey and distilled water for ur median?

[@almcm8120]

@@jasonpelton7252 good luck it worked very well 4 me!

[@yung_god_official740]

What kind of water did you use was it distilled ? Purified or tap?

[@mattborski]

It doesn't matter that much since she sterilized the liquid, but I always prefer to use distilled water to keep lead and other minerals out.

[@rekamwaktu]

Great simple :) What size for the airlock?

[@johanhallgren]

What gauge size are you using for the needles? And how long are they?

[@Stream-o-hacker]

Really good work i had many troubles with my spawn bags... because it was always too hot, i think ur doint this since a while xD

[@davidmireles9774]

Instead of inoculating your liquid culture from an actual fresh mushroom picked from harvest, can’t you also inoculate using mycelium that you grew on an agar petri dish? Would all the steps be the same except for the what your syringing (viz your Petri dishes’s mycelium as opposed to a freshly picked split base of a mushroom)? Thank you and great channel!

[@merct2894]

It should work as long as youre using sterile technique. So, yeah

[@davidmireles9774]

@@merct2894 thanks

[@Dval-rm1px]

How long can you keep the jar and the lids full of stuff and should they be placed somewhere cold like in a fridge?

[@myco_chaotix]

Ive seen many say 3-6 months in dark room temp place; but u gotta check its virility every month or two on a agar plate as mycelium eventually does experience senescence.

[@markking7290]

Do you use the silicone gaskets that come with the plastic lids? If you use traditional metal lids, do you use them and screw on the lid cap????

[@martonkocsag8324]

What about the preparation (sterilization) of the cereal medium?

[@markrawson1435]

thanks for the links, makes it so easy, love it

[@Keyit0]

Hi Ashley, can the pressure cooker step be replaced by just putting the jars in boiling water?

[@BoomerShroomer]

No.

[@justinpearce741]

G'day Ashley Just wondering, would this still work with a dried mushroom?

[@mattborski]

Did you dry it with a dehydrator? Probably not. Heat kills spores.

[@KitPhuket-ix1en]

Where did you buy the syringe, and what size is the needle? I couldn't find that type of syringe and that needle size anywhere.

[@josuecornejoponce]

Can I use a steam sterilizer instead of a pressure cooker?

[@shardj5151]

For LC is there any reason you need a gas exchange filter rather than just using micropore tape?

[@rueben1000]

Hey, Great video! Is the magnetic stirrer completely necessary? Or does it make it a bit easier?

[@BoomerShroomer]

Just makes it easier to stir and gets completely dissolved. Not necessary. Thank you!

[@davidc.3145]

Some people use a few small pieces of broken glass in the jar, (placed before pressure cooking) to help break up the mycelium, if they don't have a magnetic stirrer .

[@Keyit0]

Hi, is the pressure cooker necessary? Can I use a steamer or just put the jar in a boiling water? Thx..

[@baldjesusIRL]

Manual shake with a marble inside is what I do as an alternative and it works well

[@iimikec]

What gauge needle did ya use. I could have sworn you said it in the video but now I can't find it 🤔

[@BoomerShroomer]

I use 14-18 gauge for LC. Big enough to suck up the big chunks.

[@ThomQafzezi]

@@BoomerShroomer how long was the needle inch wise? Having a hard time finding anything longer than 1.5 inch without being a blunt end. Thanks in advance. Love your videos.

[@icy7136]

THANKS ASHLEY! AWESOME VID!

[@pacificcoasthiking]

Is that a 5” blunt tip needle? How does that needle work in the rubber injection port? If I am getting contaminated LC jars is it more likely from the fae tape or the gasket material port when I am inoculating and getting the liquid all over the inside port area? Thank you for your work and experience

[@spygear55]

Are the lids tightly sealed when pressure cooking?

[@michellefitzgerald873]

When making agar can i use just agar powder or must I use malt extract too?

[@annaioannidi7281]

hey there - awesome video - thanks for sharing I have a question - do you keep the jar in a dark place while the mycelium is growing during these 7 days or not? Light affects growth at this stage or not? thank you very much

[@BoomerShroomer]

You do not need to keep the jar in a dark place just out of direct sunlight. Light does not affect the growth because they do not have chlorophyll.

[@jozsefhideg5166]

What about spore into the liquid culture...could it work? Ty

[@LtJAS]

Nice! Thank-you! In what environment/temperature do you store the bag, please?

[@BoomerShroomer]

I store them in my office. It is about 70-75F in there most of the time.

[@melissaguffey3226]

Do you need to use the entire culture syringe in one bag?

[@anthonyc9859]

I want to echo everyone's thanks. These videos are excellent. Regarding breaking and shaking the bag, can you do this too often and if yes how many times is enough, 1,2,3...? Thanks

[@ghostviper3935]

2 is more than enough, anything over that and you might risk killing the mycelium or putting it in stasis. I usually only need to shake once at around 35-50%

[@anthonyc9859]

@@ghostviper3935 TU!

[@queerjay1335]

Are they placed in 33-36 f or fridge temp? Are they stored at room temp alongside monotubs? Does putting it in the fridge make it go dormant like it would a grain cake?

[@a.wilkins1708]

Any extra light what extract? I live in Sweden, I don't have access to the ready mixes so need to know how I can mix the medium myself. Sweden is still in the dark ages in much respect...

[@7-ten]

Light malt extract. Hope this helps.

[@a.wilkins1708]

@@7-ten thank you so much!

[@Zack-ul9js]

Hey thanks for the video! I have a question though. Say I buy a oyster mushroom liquid culture online. Can I inject that into my own liquid culture mix and have it duplicate? Thank you!