How I Turn a 10cc Spore Syringe into 310cc's of Mycelium Liquid Culture to Grow Mushrooms at Home

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Spores aren't cheap! I use this liquid culture procedure to stretch my 10cc spore syringe into 310cc's of liquid culture.
A copy of the written procedure is located here: oneearthmushrooms.com/pages/l...

[@MycoDogs]

Nice tutorial i like the recipe, just a few comments on your other processes. Why did you inject an entire 10cc spore syringe its a waste of spores you could have added a few drops, and did you qc the syringe on agar before to make sure its not contaminated? Also at the end you ruin the sterility of your LC when you add it to the syringe in a few ways. Firstly, assuming you autoclaved the LC syringe, you negated that by placing it on non sterile surfaces, then spraying it with a sanitizer(spraying iso on a sterile object makes it sanitary and no longer sterile, 2 very different things). Second when you pull the sanitizer into the syringe you make the inside of the syringe no longer sterile. Lastly when you run the needle through the micropore tape you expose a large hole to open air then put your fingers all over it. Not trying to hate or anything a lot of people dont have the air quality to work like this without contaminations is all. Here's what I'd do differently: prep: Autoclave the LC syringe in tin foil and keep it wrapped until ready. Use a RTV injection port (you can stab those thick blunt tip LC needles thru it many times) process: Operating in a flow hood or still air box. Prepare by exposing the connection end of the syringe and place it down not touching any surfaces. Open the syringe and slide it half way out of the package, remove cap inside sterile package. Remove syringe completely and attach it to sterile needle by holding the needle from the tin foil making sure never to touch it. You can flame sterilize the needle but its not necessary if you pressure cooked it well. Wipe and spray 70% iso on RTV port. Put syringe in RTV injection port and suck up ur LC. When you finish pull the needle out(the jar remains sterile). Remove needle, you can touch it now. Put syringe in sterile package and secure the cap inside of it without ever touching it.

[@headonswivel7569]

I had the same response

[@OneEarthMushrooms]

I wish I could pin this to the top! Great advice, and I hope more people can see it in the comments. This will definitely help folks have a lower contamination rate. With the technique (or lack thereof) I'm using, I've been experiencing pretty low contamination rate, ~15%. That's not ideal, but it's working great for me and I'm okay with losing a jar every now and again. Appreciate your response and advice!

[@OneEarthMushrooms]

Update: I figured out how to pin your post!

[@MycoDogs]

@@OneEarthMushrooms 15% is decent but if u lived in some of the houses i've lived in it would be up to like 80% it really depends on ur environment and a lot of other factors like if outdoor molds are sporulating that season and stuff, im glad its working for you but im sure you could get ur contam rates close to 0% if you practiced a bit more sterile technique. I like to watch biochem lab demonstration videos on sterile technique as they've been doing it for hundreds of years in that field

[@roberttomsiii3728]

@@MycoDogs I appreciate the time you've taken to provide us all with some additional Qualty Control processes. I could see where if I were just providing my own culture just for me the extra labor and processing time doesn't sound high value for a 15% failure rate on a easily scaled system. I would definitely be following your recommendations at any point where other people are being provided that LC or consistency in my results are mission critical. In any case I will be taking your recommendation and looking into sterile lab techniques mostly out of curiosity but also to have that information available in my pocket. 👍👍Thanks.

[@SusanaGomez-mp8sk]

Psilocybin mushrooms are just filled with so many health benefits. Psilocybin mushrooms treatment saved my life from depression and excessive alcohol use. Never thought I would be saying this about mushrooms.

[@Bastianbishops]

Can you help with the reliable source I would really appreciate it. Many people talk about mushrooms and psychedelics but nobody talks about where to get them. Very hard to get a reliable source here in Germany. Really need!

[@BrownGeorge-pw2xo]

YES very sure of Dr.benfungi. I have the same experience with anxiety, depression, PTSD and addiction and Mushrooms definitely made a huge huge difference to why am clean today.

[@NicoleCtirad]

I hate that psilocybin gets grouped with drugs like cocaine and heroin. Mushrooms are a remedy, not a vice!

[@ChristopherEric-fr8im]

How do I reach out to him? Is he on insta

[@PriscillaLogan-by9ll]

Yes he's Dr.benfungi.Lsd and psilocybin are amazing teachers along its dmt mah dudes have safe trips all. Shrooms are blessings from nature.

[@TechGorilla1987]

I got a 10cc syringe from a reputable dealer. I have used a grand total of 4cc from the syringe and in return I have 10 flourishing, clean agar plates and 3 quarts of popcorn inoculated. The remaining 6cc in the syringe is so dense with spores it's amazing. The agar plates are for isolating strong specimens, then the LC comes from my favorite from that. For a $21 syringe, you can do a WHOLE LOT. For sheer ghetto points, I have never even screwed the provided syringe on. I have used 4cc of spores completely bareback from the lurelock port. I observe reasonable aseptic techniques but do not have a laminar flow system. None of the 10 agar plates are contaminated.

[@omarlittle5802]

Ghetto points awarded! 😂

[@ilovecannabisalot7324]

@@omarlittle5802 10 clean agar plates noen of em dirty and thats ghetto? here come get ur stupid award lol 🤪

[@starfishandroid]

Thank you Jared for the All in one grow kit. Blessings to you and your family❤

[@OneEarthMushrooms]

Thank you! Blessings to you as well :)

[@danielalt7508]

3:00 Candymaker has a tip for you: Sugar starts to crystalize if you only use one type of sugar with water and heat - no matter which sugar you use (glucose, sucrose, fructose). Corn syrup contains only glucose sugar (usually something like 30% of weight is glucose while the other is water). If you mix glucose with sucrose (in your case something like 1-2g sucrose into the 12g syrup as around 3-4g of the syrup is glucose), it will not crystalize and you have not to worry about it.

[@metarox69]

what is the fundemantals behind this?, i am very curious

[@danielalt7508]

​@@metarox69 It comes to the molecular level of the sugar. As sugar (fructose, glucose, sucrose, lactose, maltose...) is a crystal molecule, and it is mixed with only the same kind, then it will be able to bond and create crystals. But as fructose and glucose for example are different molecules, they will not be able to form a bond with each other, and therefore no crystallization. When you heat sugar, it vibrates and breaks out of the regular crystal form. Mixing in another different type of sugar molecule and they will mix together as any liquid will. When the mixture loses heat, the crystallization begins to form. If you cool down the mixture very quickly, the molecules will be mixed together and they do not have enough time to separate. Therefore crystals will not form. But if the mixture is slowly cooled down (over hours or days), the molecules will have time to find each other. The molecules are attracted to the same kind and will find their way to form a strong crystal bond. The longer it takes for the mixture to cool, the bigger the crystals will become, because the more molecules there are to attract to each other (this is how rock-candy is made for example). There is an article where the writer explains this with good illustrations. You can find it by searching "the sweet science of candymaking" by "Tom Husband", published in the website "ACS". I hope this explained roughly how the sugars work.

[@metarox69]

@@danielalt7508 Very nice to know the answer but is not usable within homemade mycology tools because almost everyone use glass a not pyrex(borosilicate glass) which is very expensive so cooling down quickly will result in breaking the regular glass container especialy in a pressure cooker when you cant release the pressure very fast for the risk of contamination and the sudden PSI drop can break the glass too

[@danielalt7508]

​@@metarox69 Very nice insights. Also I just notice I wrote a little bit wrong on the previous message. Paragraph 3 is not meant to refer to a mixture of different sugars, but instead to a mixture of water and one sugar... I must have written that in confusion on the usage of the word "mixture". Basically having 2 different sugars in one mixture, will never form crystals until a very long time has passed and the mixture is left untouched (for example honey that goes from liquid to solid). The rock candy is just one type of sugar (usually sucrose) mixed with water, and then cooled over long period of time. This means that having a sugar combination of 2 different sugar types in an autoclave is not going to crystallize. -------------- If I remember correctly, the mycelium absorbes easily monosaccharides (just like humans), which are fructose, glucose and galactose. The ideal sugar mixture would be a combination of at least 2 of these sugars for the mycelium, and mixing those will prevent crystallization.

[@metarox69]

@@danielalt7508 nice to know, will keep in mind when i have the best genetics from my APE ( probably years)

[@bartholomewnathaniel807]

As I am just starting out in this endeavor your video simplifies what I had always imagined could and is being done- thank you!

[@lachyj8344]

Your channel is the best on KZbin. We love you!

[@adamhumphreys8333]

Brother, your videos are fantastic, informative and straight to the point! Thank you for all you do! ❤

[@csarebel8657]

Easiest, most straightforward video ive seen yet.🙏🏻

[@journeytoascension7018]

Yes the easiest way to get you a contaminated culture. Goof luck with the lack of knowledge. Never just watch ONE video and think you "got it down". You will waste time and money watching videos from amateur growers like this.

[@justinblackburn649]

So helpful for a noob like me. You’re the man!

[@jakethemedic1]

Give yourself another gift: If you tab the tape on the roll when you're done, it makes it easier to pull for next time, plus you already have your tab ready to go! Great videos and love your calm energy, thank you!

[@RhianChambers]

Thank u so much this looks so much simpler than I had anticipated

[@inyoureyes9197]

This is the only video I've watched like this! Thank you!!

[@OneEarthMushrooms]

Well thank you! Glad you enjoyed it!

[@TomofAllTrades]

Thanks for sharing this concise and informative coni👍🏼

[@brandonsamson6278]

Nice work! Getting ready to do some of these myself and the information is very appreciated.

[@lordmozz_trip]

You can easily purchase from this specialist I usually order from on Instagram or telegram as..

[@lordmozz_trip]

Levine_tripp¿?

[@MyTube4Utoo]

Very interesting. I'm just starting to learn all this stuff and that was very helpful. Thank you.

[@kristinacoulthread6600]

I am just learning also after using kits for a while

[@TheQuest888]

Thanks for the rad work!

[@thinkaboutwhy]

Thank you, using your website. Enjoyed the video, like your style.

[@_.KLYDE._]

You seem pretty chill. Going to check out more of your videos.

[@anonharingenamn]

You made it look so easy!!

[@HollywoodMoonshine]

Awesome video! Thank you for the lesson!

[@00wop80]

Thanks. I had given up on trying to cultivate at home until watching this.

[@potassiumc]

Thank you for reporting in grams and millimeters. :)

[@electronicaeasy]

You crack me the hell up, love you broski.❤

[@Natouk01]

What a great pedagogue you are. Can't stop watching your videos. Little question here. Where (i guess fridge) and how long does your liquid culture keep?

[@OneEarthMushrooms]

Thank you, Nathalie! Correct, the fridge is where I keep the culture. I've kept them for up to 6 months in the fridge, though they may last longer. Six months is the arbitrary cutoff I've given myself to make sure they are viable when I inoculate.

[@paulrush9166]

Really great hair day for sure bro. That's really all that matters in life

[@clintwestwood1895]

Cool video bro, live culture rocks!

[@girththeogre]

Thank you friend! This is important information!!!!!!💚 "Thank you for watching." Thank you for making!!!!!!!!!!!!!lol

[@brianbordenkircher52]

Very helpful! Thanks!

[@solocede]

Thank you so much, great video

[@CasualGamerNoob]

bro thats easy af, thank you!

[@MycoJuice]

Very informative video, very helpful. 🙏🏾🍄

[@OneEarthMushrooms]

Awww thanks! Glad you found it helpful :)

[@titoondeck978]

Great video 🤘

[@OneEarthMushrooms]

Thank you!

[@d.o.t.collector4728]

@@OneEarthMushrooms Need a little help please. My spore syringe liquid is clear, I've heard you're supposed to see little black dots or it should be cloudy? Is this correct? Second, I hear using a liquid culture syringe is much faster and a lot more successful? Any help would be much appreciated 👍👍 Thanks

[@sterlingstrange6064]

Great channel.

[@ryanarmishaw3305]

@4:35 - that is the most beautiful Snow Globe ever :)

[@Letsgotoluna]

You are a rare breed on KZbin, sir. Your videos are direct, not gimmicky, easy to follow, and delivered in a professional manner. Thank you for your efforts. One question. What gauge is the blunt 4 inch needle?

[@OneEarthMushrooms]

Well! Thank you for the kind words! The blunt tips are 12ga.

[@MadScientist816]

Hey brother Love your channel Thanx for all the information you provide Also busy with my own cannabis and mushies channel Mad love bro Peace

[@kuksiBG]

+1 subscriber my man! good work

[@OneEarthMushrooms]

Awesome, thanks!

[@llllllllllilillii]

Thank you

[@carloscardenas5413]

Thank you 🙏🏻

[@MadScientist816]

Hey brother Hope you are well Just wanted to tell you that your channel inspired me a lot I'm also trying to start my own channel now growing cannabis and mushies Mad luv Peace Mad scientist

[@jeffreyhalverson2611]

Ya man, you look fabulous! grin.

[@jonathan-smith]

Awesome vid

[@LowTierAutist]

Thanks

[@Mong00se22]

Thankyou

[@peaceseeker123]

Rock on buddy thank you

[@lunarflares2659]

Thank you!

[@OneEarthMushrooms]

You're welcome!

[@SHITS2DGIGGLES]

Wow.. thankyou.

[@MrYusha]

LME liquid malt extract will show growth in a couple of days, i used to use karo but LME is a game changer!

[@floso1916]

I loved the proprietary joke 😂😂😂

[@waynesvan4676]

Thank you for sharing. off to have a mooch on your site..

[@joelwhite3591]

I'm getting mine from @benjidelice

[@BadddDoggg-id4po]

Just found you, I subbed!

[@jeff3809]

It takes 1 to 3 weeks for lc to colonize. Thanks for the updated steps!

[@FantomFirez]

No it doesn't, it can take as little as 3 days

[@RNG-999]

@@FantomFirez Multispore Syringe to Liquid Culture will take 10-21 days. Agar to Liquid Culture or Liquid Culture to Liquid Culture will take as soon as 3-5 days. Multispore Syringes have to germinate first. Then after germination, they have to find other nearby spores through sending hyphal threads outwards in search. Multispore Syringes as a result take 2 - 3 times as long to colonize than any other method. And your Multispore Syringe/the spore print that was used to create said Multispore Syringe could have had contamination that you will not have noticed until very far later and very much further down the pipeline of growing, nearing fruiting conditions or right after spawning to bulk substrate.

[@ruth1486]

thank you

[@OneEarthMushrooms]

You're welcome!

[@dr2be50]

Just ordered my starter kit. Been looking for something like this.

[@OneEarthMushrooms]

Heck yeah! Thank you, shipping out first thing in the morning!

[@reklezzz9038]

@Hoffman Jeck be careeeful bruh😂 idk where you’re from, I’m from texas, the drug war still going STRONG here. Used to sell weed lsd and shrooms, meth eventually when money was tight(it was the most popular at the motel I lived in) Had best friends planted in my life, informants setting me up with cheaaap felonies. My advice if you’re gonna advertise and try to boost your clientele, stayyyy moving. Move around a lot. Hard to hit a moving target.

[@carolday3381]

Just wanted to add on the contamination worries especially for us first rate newbies,.. if its too overwhelming to make mason jar blenders and opening jars etc,.. make the liquid substrate jar with an injection port,.. and inject bought liquid syringes. This is not to knock the poster’s skills,.. i just find this video very advanced compared to where i am at this point in time. Also i live in a 200 year old house from hell (building a new place thankfully) but theres every possible way to contaminate in this house i am in right now. It would increase my chances of success if i bought a pre done spring and injected it into a sterile jar of liquid culture. I can learn to make that jar, to add nutrient and inject sterile air etc. but making the Petri dish, cutting it etc thats a ton of opportunity to add contaminants when done by me😊.

[@SnufflePig]

great videa dude

[@OneEarthMushrooms]

Thank you!

[@aaronsmith2054]

Awesome video dude! Question, I want to do this but syringe is a live culture syringe with mycelium in it instead of spores. Can I do the same process and just inject the jar with the mycelium I already have instead of spores?

[@TimothyChibueze-pk7hv]

THIS IS ONE THE BEST CHANNEL I HAVE COME IN CONTACT , PLEASE TELL US THE NUTRIENT YOUR USING . IS IT LME PDA SYRUP HONEY PEPTONE??? REPLY BEFORE I MAKE MISTAKE THANKS AND REAMEN BLESSED

[@chuckcharlebois4199]

Hi. I just found you and have enjoyed the videos I’ve seen so far. I’ve also checked out your website. I’m very new but already looking ahead. I was wondering was size your pressure cooker is. I will certainly be a customer on your website.

[@OneEarthMushrooms]

Hello Chuck! The pressure cooker I use is a 23 quart Presto. I bought this one at Ace Hardware (I'm assuming you're in Canada, and I'm not sure if they have Ace there). Thanks for watching and for the kind words!

[@curtis026]

Hahaha 😂 dude is WRECK’T! I love it

[@grooty_yop1055]

Thanks for this awesome video, I gotta question, can I use Honey instead of kernal syrup ? If not, what else can I use in case I dont find kernel syrup in my country please

[@johnnyt7900]

Cool 🎉

[@cameroncarter8620]

Thanks for the videos im on 15days n there is still alot of spores in my jars did i do something wrong infollowed everything to the t

[@mr.m.o.g.o.m.]

Did u make the lions mane harvest video from two years ago?

[@ianpardee7505]

do instant pots work in place of a standard pressure cooker?

[@PutHimOnBlast]

Hey man! Is the rock special or is it just for mixing the LC around instead of using a magnetic mixer?

[@meldeviaedit7478]

Did you use a cabinet against air germs?

[@DiscoStuLikesDiscoMusic]

What are the "nutrients" u inject in jar prior to pressure cooker process?

[@CidFFVII]

Folding the tape is called a buddy tag.

[@randymetz2616]

Can you transfer a grow bag with mycelium to a monobox with substrate? Ty

[@1ntwndrboy198]

I would like to see you test with using biochar in the substrate. You would probably have to innoculate it with the rest of substrate. Still think it would be beneficial, especially on multiple yields.😁

[@OneEarthMushrooms]

hmmm biochar, eh? I have a big bag of charcoal sitting in the shed that I intended to use for biochar. Are you suggesting adding biochar to the liquid culture?

[@questioneverything4633]

@@OneEarthMushrooms I think he means adding it to the substrate as a good source.

[@cristinacassidy604]

Your hair really does look excellent today 😂

[@dand7056]

Nice hair. So do you see contamination in the LC or do you just roll the dice? Is it safe to assume that the same thing can be done from LC to LC? How manc CC of LC would you add to 800ml of solution?

[@TimothyChibueze-pk7hv]

GOOD LUCK TO YOUR WORK SIR, AM ABOUT TO START NOW , THE NUTRIENT YOUR USING IS IT LMA OR AGGA AGA. NUTRIENT AGGA ? PLEASE REPLY BEFORE I MIX . THANKS AND REAMEN BLESSED

[@bagchaser77]

What kinda nutrients do you use in that first syringe?

[@CCPW_chicago]

Do you admin 10cc liq cult into 5 lb all-in-one. The same as you would 10cc spore?

[@262Purity]

Would Senescence occur the same way as grain 2 grain does?

[@joshhawn3988]

bro look lit af in the begining.

[@davidhoffmann7710]

What exactly are the nutriants you are adding to the water?

[@dineshmunthry854]

If I want to use peptone and malt extract how much of each do i use?

[@S-C-O-R-P-I-O]

Can you use jelly powder(no gelatin) instead of agar powder in Petridish? Pls reply fast

[@danielodonnell6334]

Awsome

[@OneEarthMushrooms]

Thank you!

[@BostonDort]

Would you knock a jar up with the whole 10cc of spores, or how much per jar? Plan to use 600ml LC.

[@emiliothechicano3231]

Can i use lc instead of spores? Just putting 10cc Liquid culture in the jar?

[@karmakaotic1803]

If u add honey or kyro syrup and place a heating pad near it u could have tripled that growth and if u used that spore syringe and did 3 different jar tripled the amount as well. Still a great video tho!

[@johnandrews9433]

@@decentwoodz5172 the corn syrup that was mention 15 seconds before that lmao 😂

[@DCsPr0ductions]

@@decentwoodz5172 literally syrup was what he added which this original commenter suggested.

[@timothymccarthy7747]

Won't too much mycelium in your syringe clog up the needle point?

[@DCsPr0ductions]

@timothymccarthy7747 no, it will be forced through, alive and okey.

[@556SMOKE]

Wdym fam im about to try and do it for the first time and I got 10ccs of spore and a pressure cooker

[@UndrSkorMusic]

What would be the steps to get the same result in an instapot?

[@neveildaniel8523]

How long can it stay in the jar before putting in syringes

[@mdoggm13093]

Great video. Thank you for your information. So now you have 10 30cc syringe where you drew mycelium (not spores in through a 12ga needle. When you are ready to dispense into spawn jars or bag,, do you have to use same sized 12ga needle? Or a more standard sized injection needle?

[@Outlier117]

I take off the 12ga blunt needle and use a 16ga pointed needle when I’m ready to use. Has worked for me pretty well.

[@area___]

what did you use as nutrients?

[@shane80122]

I had an issue or problem that maybe someone has suggestions for. I had two setups, identical to yours in a pressure cooker, and water boiling at 20 psi (vs 15) caused rock to jump around tapping the bottom of the jar and splitting the bottom off both 312mg setups. Just like tapping glass for a controlled break the bottom was 360 tapped and broke. Any suggestions other than not have a rock in there? Great sterile technique. My water level was a little deeper than yours and just touched the glass bottom

[@KB-Reigns]

In order to get mycelium growing the quickest… is it faster to turn spore syringes into LC rather than just inoculating my grain with the spore syringe?

[@johnbauby6612]

Really great vids. I like your approach.

[@OneEarthMushrooms]

Thank you, John!

[@johnbauby6612]

@@OneEarthMushrooms Right on. The simple approach is best. I prefer your straight forward and simple but well made vids over the overly produced ones. Hey, how do you like shopify? Does it work well for you?

[@OneEarthMushrooms]

@@johnbauby6612 Shopify is pretty great for me because I don't know much about web design (and frankly, am not very artistic when it comes to designing anything). Pretty much anyone could easily use Shopify. Of course, you have to pay for all of that, but it feels worth it to me. Are you considering starting a website?

[@johnbauby6612]

@@OneEarthMushrooms Thanks for your reply. I "kinda" use Etsy because they take all the hassle out of having a website but even Etsy who claims to be "artsy" friendly is just a money whore that nickle and dimes you to death so I have been looking into my own site. I do a lot of woodworking and glass blowing. I guess we have to pay one way or the other. It just really burns me when these companies make billions for doing next to nothing off of the hard work of everyone else. I again thank you for your very helpful , educational and entertaining vids!

[@greasyweeds861]

Hey there is RO water ok to use?

[@jeffsmith8065]

What type of syringes do you order?

[@LoveWins]

I've got a question, Would this work by using a 💉 of liquid culture? Or does it have to be done with spores only? Also, I wish you sold liquid culture on your site, I'm in the market for some as I am about to do some research pretty soon. ❤

[@patrikholoch7547]

Does the alcohol not harm the Micelium or the Spores?

[@riley8429]

What is the cleaner youre using?

[@Mo.English247]

Can I use another sugar instead of the corn syrup?

[@randymetz2616]

New to this can mycelium look like powder in the grow bag I'm having trouble growing mycelium