Cloning plants using tissue culture propagation

I have been curious about the tissue culture propagation for some time and today we dive into it with special guest Jeremy Spath of Hidden Agave Nursery where we take a brief look into how he operates his boutique agave nurseries laboratory. One thing that has fascinated me about the plants in nature compared to the trade and hobby is that in nature the morphological variability is all over the map within plant populations. When you go to your local nursery things tend to look pretty cookie cutter. Big box nurseries use tissue culture to propagate large scale clones of popular selections. Whereas here at Hidden Agave Nursery, Jeremy is using it to propagate his unique and unusual agave hybrids which allows him to make small batches of his exquisite selections of his in house hybrids. Super cool to see a agave that grows in Oaxaca Mexico hybrid with an Agave that is native to Californias Mojave desert. I hope you enjoy the video please consider becoming a subscriber and if you'd like to help support the channel you can also become a supporter on patreon.
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Hidden Agave Nursery - Jeremy Spath @HiddenAgave
hiddenagave.com
Thank you so much for watching the video I appreciate you!
THE QUEST CONTINUES.....

[@phonphon2130]

All of agaves have shown in this vid are super super gorgeous. 🤩🤩🤩

[@KillsBillions]

Some of those terminal spines are absolutely insane. So beautiful

[@unknownsucculents4845]

This was my first introduction to tissue culture and amazing to see how it works while being presented by a legend, Jeremy Spath.

[@JoseFloresEC]

I’ve heard of tissue culture propagation but never understood how it was done so this was a great video. Definitely a clever way for cloning plants for home settings and those in high demand and threatened by poachers in their native habitat.

[@tcss0612]

cactus can use single areole as explant. more over since cactus flower are actuelly containing stem tissue and therfor areole. you can use cactus flower bud as explant. it;s the perfect explant for cactus TC.

[@huckwild6464]

i love how with all blue heelers fetch is a life or death game not just for fun haha

[@cactusjoe]

Nice video and interesting to see this methodology being applied to cacti and other succulent plants; has of course been used for mass propagating of so many other plants, including Orchids, for decades.

[@Tminus89]

Tissue culture is a very interesting process, loved to learn some more about it :) In an earlier video, I said to you I didn't like cacti and succulents enough to want to own them (yet) but found them very interesting... since I have been seeing more and more varieties and I am now sold haha. Bought 2 small cacti and put some marvelous Agave on my wishlist! Will keep following you on your plant journey

[@slashfeint]

Thanks for sharing this with us! This guy is obviously a pro and knows his stuff.

[@bobs5596]

he goes by 'Heisenberg'...

[@ChopsticksDIYGarden]

Looks like a nice guy. He talks and looks like my neighbor.

[@BelgianSpeedcuber]

That's an amazing way to propagate 😮😮😮 Thanks for sharing your wisdom in all your videos!! You're a true legend 🤘🤘💚

[@TerryJent]

Welcome to my laboratory! Nice!

[@crashnebula7]

He needs to update his website more often because I want to buy some but most of the cool stuff is sold out

[@billyjack3580]

He has some amazing seedlings in his catalog. Just know that when you buy a (this x that) seedling you’ll get a random seedling from the batch, not necessarily as nice as the one he selected for the image on the website. On the other hand, if he lists a named clone, like something really special that he’s selected and tissue cultured, the plant you get will look like the picture.

[@chuckgoecke]

Tissue culture cloning gets a little tricky when dealing with variegated plants and similarly chimera plants, which variegated plants kind of are. Often to come true, as in a variegated variety needs to sprout a true pup, it needs to come off the stem. Getting a clump of baby plants that are maintaining the variegation is the trick.

[@ryanisennock7049]

As someone who is super interested in doing tissue culture at home, this is a super cool video! Thanks for making it

[@southwestnomad9978]

amazing video. i didn’t know TC was a thing with cacti. i come from mycology and tissue culture is something i’m very familiar with. just so y’all know you can achieve the same results building and using a Still air box (S.A.B) for your clean environment work space. most of us can’t afford a flow hood lol. however it’s not hard, and if you can do this you’re sterile enough to grow mushrooms so kill two birds with one stone. you can also sterilize everything with a pressure cooker. much love.

[@HOODBOSS5000]

Major inspo.

[@Ascendingtom]

great to see! i have watched quite a few of Plants in Jars on youtube. She does really detailed videos on tissue culture at home mostly for houseplants

[@LarsLarsen77]

I watch her too but she has no idea what she's doing, she did it once and almost cut her finger off. What is it about youtube that makes total beginners try to teach the world?

[@AliAtapour_ProjectReef]

Another bad ass video

[@bryenanthony1890]

“You could put a dog turd in there and it will be sterile” 😂

[@jfjf-yn6wj]

you are goated mate

[@LarsLarsen77]

I've got a tissue culture lab in my kitchen but I've been struggling with cacti.

[@lostolli7766]

That's a cool video. I am getting into TC right now myself and I have gained some experience with Rhododendron sp. Gotta start to try some cacti. Btw, theoretically you could grow thousands of plants with one single cell - not even a meristem cell. In practice, you really do not need meristem tissue, but you should have some cells at least... but if you can, you are using meristem tissue. And you can think of tissue culture as something as a graft to be honest. Sure, sterility is a big pain, but you are supporting some tissue with sugar, plant growth hormones and water as well as some nutrients in both cases, but TC is more efficient.

[@lenovothinkpad8275]

I've seen Indonesian doing this with Aroids but for agaves , I never think that ........ Gorgeous plants he has , yeah 😍😍

[@jakobsulzer4831]

@cactifanatici is Socal based and does really cool TC with cacti! I'd personally love to see a Cactus Quest x CactiFanatici collab video!

[@billyjack3580]

<a href="#" class="seekto" data-time="436">7:16</a> This has to be a Dyckia hybrid.

[@thenickspohn]

What is the color coding mean in the media and what are you using to color?

[@jeffbennett-nw3cb]

I'm also curious as to the significance of the different coloured growing media @CactusQuest can you relay the question to Jeremy?

[@scottsolar5884]

I have been buying seeds and chemistry to do exactly this!

[@Jen-br5hx]

no chemistry required dude.

[@bobs5596]

look at us man! can you believe it? we 're still using SEEDS!! arrrgh!! i want my neon red and green fluorescent blinking arios NOW!!

[@MR-puffnstuff]

What is your web site for ordering information and so fourth please.

[@dj33036]

I suspect they're doing this with animal life as well.

[@wilsonb5417]

Oh it's already been done. Hundreds of animals have been cloned but scientist haven't perfected it yet because they don't live very long.

[@brianfitch5469]

@@wilsonb5417 ya they do, you can get your pets cloned for like 50k now. And live a average life span.

[@leechaille121]

Nice video! If you are interested in touring a commercial TC lab let me know.

[@MNtwincitieswaters]

Autoclave sterilizes everything except infectious prions

[@alpidiasalani3058]

È lo stesso sistema con cui moltiplicano le orchidee .

[@helgedonath1524]

why do you do something like that? it's like frankenstein. it no longer smells like nature and the desert. it smells like a laboratory and disinfectants.

[@bobs5596]

do you eat strawberries? they are most likely from tissue culture...

[@brianfitch5469]

It would be a faster way to propagate cactuses. With a lot of cactuses you can cut the tops off replant the tops and the base will pup out multiple new heads that once big enough could be cut and replanted and are exact clones of the parent plant. I do it with my trichocereus strains. If your needing clones for a commercial operation to sell this would grow a single plant much faster.