Citrate Test

Biology Professor (Twitter: @DrWhitneyHolden) describes the steps of the Citrate Test, a method to differentiate bacteria in a laboratory based on their ability to use citrate as a carbon source. The lesson includes the components of Simmons Citrate Agar and an explanation of how a pH indicator results in the color change observed in a positive reaction.
Want a PDF of the final white board with all the answers written in? Download FREE STUDY NOTES here: tinyurl.com/Bi...

[@oksanacyhanukova5228]

Thank you so much for your videos! It helps me a lot to understand the process of each test, in comparasant to the script that we received.

[@BiologyProfessor]

You're very welcome!

[@ibrahimabdalla2567]

Thank you for all that great effort

[@weirdestweird3369]

Ur voice makes me understand fast

[@SauravKumar-xf6tn]

U teach very well. Best microbiology teacher

[@prathapagiriharish2687]

Nice explanation mam 😊

[@rubyfoster198]

May I ask a question? Is there any possibility that Escherichia coli having a positive results in Citrate? If anyone know, please kindly answer my question. Thanks a lot!

[@ivanbombana7282]

Have you gas your answer? I think yes, but I' m not sure

[@rubyfoster198]

@@ivanbombana7282 yes, it can be postivie in a certain E. coli strain. But, thanks!

[@korea45742]

Can you explain or give document to know why When inoculating multiple biochemicals from the same culture, inoculate citrate test media first, or flame inoculating needle prior to streaking this medium ..

[@BiologyProfessor]

It is because if you inoculate another medium first that has glucose or similar sugars in it, and then accidentally transmit some of that glucose or other chemical over into the citrate agar, it can give a false-positive.

[@janaan6973]

Thanks Professor!

[@BiologyProfessor]

Thanks for watching :)

[@lamismemish4957]

THANKS!

[@mayanksangam9205]

Thank you professor :)

[@BiologyProfessor]

You are welcome (again)!

[@samahahmed1673]

Thank you so much

[@BiologyProfessor]

You are welcome!

[@gurleenvlogs6731]

Thanks mam

[@alejandrinapadilla7188]

does Bacillus megaterium could cause a positive result?

[@BiologyProfessor]

Yes, I believe so.

[@ivanbombana7282]

What Is/are the reaction/s of this test?

[@BiologyProfessor]

More info here: microbeonline.com/citrate-utilization-test-principle-procedure-expected-results-and-positive-organisms/

[@harshaddave857]

Will you please reveal how did you lose your weight???? ......Anyway Teaching Great....... looking Beautiful.......

[@BiologyProfessor]

A ketogenic diet - it is amazing!

[@harshaddave857]

Thanks for kind reply....

[@agutierrez342]

Thanks!!

[@arjupalikhey]

Oh my dear lord, you have lost so much weight and I'm still the same. Great job!

[@leewilliam3417]

Mmmm😊