Colocalization lecture

Presentation and materials for exercises available here:
doi.org/10.176...
What can colocalization refer to? (0:48)
Need resolution to interpret colocalization (4:26)
Nomeclature (7:56)
Imaging and pre-processing decisions (9:10)
Quick check - yellow or white (17:27)
Quick check - line profile (20:11)
Quick check - 2D histogram (22:21)
Colocalization metrics (25:31)
Intensity correlation - Pearson's (27:36)
Intensity correlation - Spearman's (30:30)
Overlapping fraction - Mander's (31:13)
Comparing different measurements (38:07)
Thresholding - manual (40:47)
Thresholding - tolerable number of false + pixels (42:06)
Thresholding - automatic global methods in FIJI (45:24)
Thresholding - automatic local methods in FIJI (46:52)
Thresholding - Costes (47:46)
Thresholding in 3D (49:45)
Thresholding consistency (50:36)
Measuring per cell or per image (52:06)
Drawing ROIs (52:25)
Comparing results between conditions (53:04)
Comparing results to "random" (54:41)
Robustness of results (57:53)
An example from Jenny Ting's lab (58:16)
Documenting results (1:01:12)
Take-home (1:02:33)
Resources (1:03:24)