Comparative genomic hybridization

[@mahnoorkhan9784]

I owe my bachelors and masters degree to you sir

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@izekesama6820]

6 years later still saving lives

[@shomusbiologyofficial]

Thank you

[@subhamoydas6837]

Exacty.... 🤘Always shomu sir👌🤘

[@piotrkopytko6542]

9 years haha *

[@indian62353]

10 years later too

[@pratikshaantil9144]

Now 9 😂

[@kefeibao96]

When I try to find some video of teaching aCGH, your lecture showed up and it's so clearly to understand the basic principle and thoughts of it, so helpful! Thank you, friend.

[@shomusbiologyofficial]

Thank you

[@Sam-ie1qv]

I have genetic exam tomorrow and your videos make it so easy thank u from the bottom of my heart. God bless you

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@kashafseducationalsite363]

sir.. i m so so thankful.. u cant understand how i m feeling relief after watching this . u cleared my all confusion.. saty blessed .. thank u. from Pakistan

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@mahamijaz6001]

I can't believe that thus video is 10 years old. We both friends are getting your lectures and didn't even realise...very efficient and effective material..thank you ❤

[@shomusbiologyofficial]

All the best for future career

[@achigwecynthrus2343]

9years later and still the best video…. Thank you

[@shomusbiologyofficial]

You're welcome

[@lizbethshellysaldivar8390]

I was having a really difficult time understanding this concept from my readings, but this one video made me understand it perfectly clear. Thank you for the informative video!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@darshinimalukar3246]

This guy and his teaching skills. So grateful (y) you help us alooooot of us. Thank you

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@matthewmillett1225]

Great explanation Shomu. Thanks for all the great work you do! I love how you also explained balanced translocation almost as an afterthought in less than a minute haha.

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@raahul1303]

Sir you are simply the best. You beat all the lecturers I've had so far

[@fayzio5282]

Thank you so so much! I have a genetic's exam soon and understanding these methods was the hardest part for me! You saved me!!! *virtual thank you hug*

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@shalinividyadharan3763]

thank you sir for making the concept so simple

[@shomusbiologyofficial]

You're welcome

[@marwabelgh7403]

you are amazing you saved my life thanks a lot from Algeria

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@nayimmoissi1820]

im a french guy but you are explaining very well so i can understand everything you are saying, thank you !

[@KuldeepSingh-fu7gl]

You are a great teacher Shomu! Your explanation was very helpful in understanding the concept.

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@Robertson284]

you surprised me with your explanation. thank you so much.

[@shomusbiologyofficial]

You're welcome. Glad you like it

[@bersah4517]

Shomu u the man. Helping us for years

[@shomusbiologyofficial]

You're welcome

[@solodesign]

Your explanation could not be better... Excellent sir!!

[@soumikdeb5436]

Very nice and clear teaching... Thank you

[@elir.393]

This guy is my hero, best teacher.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@melvinmesekansalar2108]

Very well understood now, thanks Shomu!

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@rimel6446]

7 years later and this is still helping us

[@malikayoussouf9406]

May Allah bless you. You have helped us so much, you put so much effort and make it so much more logical. Thank you so much.

[@zinhninhtet157]

Thank you for the video. Good explanation.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@selsabilfattouche4337]

Thank u sir after 7 years

[@Austin-xx4cs]

Thank you man .. appreciate the demonstration .. really made it easy for me

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@YourLocalIndianSamosa]

Very nice video, briefly and yet very nicely explaining the whole concept ...Thank you for uploading in such a video. I learned a lot from it !!!!

[@Happy_Rocks]

Awesome illustration !! made it easy to understand

[@wafimalik7355]

thank you sir... u saved my live

[@shomusbiologyofficial]

You're welcome

[@DoctorKenji]

Made it so easy to understand, thank you!

[@aigbekaenraymond6049]

Really helpful. Many thanks

[@shomusbiologyofficial]

You're welcome

[@hafsakhalid8503]

Thank u..simple and crystal clear

[@shomusbiologyofficial]

You're welcome

[@SuperYaniv12]

Awesome explanation! thank you very much sir!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@CopperKettle]

Thank you! Cheers from Yekaterinburg, Russia!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@ridwansyahfkuii]

nice explanation, thank you sir

[@shomusbiologyofficial]

You're welcome

[@mohammedabbaskhan2127]

TY Shomu Daa🙂

[@shomusbiologyofficial]

You're welcome

[@nahlaomar9072]

😲😲😲Mann this guys teachin..CGH was always difficult for me until God showed me this Mann...PS: i hav completed my MD pathology

[@manjuchauhan5280]

Too much helpful .ur all videos are the best 👍👍

[@shomusbiologyofficial]

+manju Chauhan thank you very much

[@sivaji-xy7ez]

Excellent explanation 👍🏻👍🏻👌👌

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@junaidminhas6984]

you are an amazing teacher !

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@m7amad2000]

That was a fantastic explanation. Thank you Sir!

[@sukh6566]

excellent video sir

[@shomusbiologyofficial]

Thank you

[@Diwakarkumar-ms7op]

Thank u so much ❤

[@shomusbiologyofficial]

You're welcome

[@nOnAmE-nl3qu]

Thank youuuu so much 🥺 you saved me 🙏

[@shomusbiologyofficial]

You're welcome

[@jonasdekeyser4916]

Thanks from belguim really good illustrated

[@markaszkenasy7395]

Nice clear explanation,thank you.

[@soulfuldreamerr]

Thank you so much!

[@shomusbiologyofficial]

You're welcome

[@bluesofi]

Very helpful! I love your videos! Thank you so much!

[@shadinasser1]

Great explanation, thanks

[@Marco-jc3ny]

Helped me so much! Thank you so much!

[@saroshkhan6259]

I'm passing this degree because of these lectures sir.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@이영선-v2r]

thank you so much! it's very helpful for a newbie like me!! :)

[@zhuoyueyang7125]

Thank you

[@shomusbiologyofficial]

You're welcome

[@drshefalinaag2701]

Nice ,informative video

[@Rjajdjdj]

Thank you sir... I understand very easily. 😊❤ Some other confuse me to acgh btw cgh

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@aqsarehman9877]

Ofcourse it's very helpful

[@shomusbiologyofficial]

You're welcome

[@farisalshboul998]

very good illustration ,,, thanks , May Allah bless you :D

[@abhishekmukherji2332]

Great job boss 👍🏼

[@shomusbiologyofficial]

Thank you

[@testtest7727]

Great video. It was really helpful. Could you make a video about Copy Number Variation(CNV)?

[@lifeandbeyond7279]

hybridization is not between the test and reference genomes (DNA), but between the microarray oligonucleotides and the both test and reference genomes (DNA).

[@zohaibamjad153]

Thank you very much sir, brilliant video :)

[@Ahsan___]

sir kal paper ha . paper sa phla apki video dekho everything clear

[@shomusbiologyofficial]

All the best

[@kamleshprajapati1482]

Thanks a lot fr explanations.. helpful it is!

[@qwerty975311]

Helped me out! Thank you!

[@SpeedSai]

Thank you so muchh❤

[@shomusbiologyofficial]

You're welcome

[@Michael-il8ls]

more advanced information would be useful. how do the data look like? what is resolution? how are data processed (examples of data files)? also how many fluoriscent probes are on each chromosome? how do mutated chromosomes hybridize each other? can they wrongly hybrydize? how many chromosomes are there from each cells? 1 or are they amplified?

[@pramitbanerjee]

the data is image files. You use certain software to quantify the fluorescence values, then you can use matlab to analyze them

[@luyingchen8454]

Thank you for the video. It is really clear and helpful. But I think there is a small problem concerning the ratio of blue: red fluorephore. First, if the blue:red = 0.75, then there is more red fluorephore, the color should be more red. But you used the blue pen to draw it.But maybe you didn't mean to correspond the pen color to the real color. Second, the second ratio of blue: red you wrote is 1:0. But I think it should be 1:1. Anyway, the video is very constructive.

[@poyosis]

What's the difference between CGH and aCGH? Is it the same thing? And when do you use the BAC vector???

[@fari1025]

thank you , very helpful

[@MuradAhmadzada]

It was helpful, thank you!

[@kardo9381]

Perfect, Thank you dude.

[@juliablaszczyk8036]

Thank you for the easy explanation of that method! :) I have only two questions: Should the value be not higher than 1,25 in case of duplication? And the same thing, should the value be not lower than 0,75 in deletion?

[@FMob2010]

Thank you! That was very helpful! :)

[@tanisarker1112]

thank u bro............. very helpfull

[@kennixcheung2270]

Fantastic!!!

[@titusmatteo3925]

very schlau Shomu!!!!

[@miladibdah]

very helpful ! thank you !!

[@ermanistifli8074]

Dear All There are two serious mistakes in this video! 1. In CGH experiments, we isolate the reference DNA from a healthy individual. But this guy argues that both the tumour and reference DNA are isolated from the same individual. This is a scientific flaw. 2. The tumour and reference DNAs are not hybridized to each other! We hybridize them on normal metaphase spreads! 3. The term that he uses "Metaphasic" does not exist. "Metaphase cells" is used instead. Please be aware that this video contains serious scientific flaw! But it is not surprising because everyone can upload videos on youtube.

[@zedor1553]

I believe that for clinical research, it is normal to extract DNA from tumor and compare this DNA with surrounding healthy tissue from the same patient. DNA from healthy tissue serves as control for studying new chromosomal abnormalities in tumor cells. I can't encounter the scientific flaw at this point, and I'd be really grateful if you could explain this point further. And, I would appreciate if you write your references if you claim that "everyone" can upload videos here. And, as well, anyone can comment on them. And "Metaphafic" is indeed an inaccurate term.

[@ermanistifli8074]

Dear Zhannie Could you please show me a study that utilizes the same individual as a source for both reference and test DNA?

[@93Royston]

Erman İstifli, Thank you pointing out the mistakes. but instead of simplying commenting down, you could tagged him in your comment. so that the mistakes could be brought to his notice.

[@nitkaigla]

Erman İstifli Good to know :) I had the same doubts watching this video

[@93Royston]

but sometimes cells from the same person are used.

[@zaramirza7745]

great video! keep up the good work!

[@sorooshghasemi7135]

helpful thanks for sharing your knowledge with us :))

[@shomusbiologyofficial]

You're welcome

[@yossora3892]

🥺 well explianed

[@shomusbiologyofficial]

Thank you

[@manjunebhani9671]

Very nice..

[@shomusbiologyofficial]

You're welcome

[@artbylupiv]

Loved it

[@shomusbiologyofficial]

Thank you

[@poojasalodiya5100]

Thanks soo much sir

[@arunimachakraborty1450]

very helpful

[@JuhiMittal]

thank you :)

[@gayatribhatt4869]

Thnkx sir

[@Yuvi_vlogs321]

Hey bro may I know what's your qualifications? I have been following you since long and saw you explaining almost all aspect..

[@ZakaToubi]

Thanks man keep on the good work ;)

[@amylatan1122]

Thanks!

[@PoonamKaushik-z6h]

Hum hybridization of genomes m ye fluroscence kyu use krege??

[@tarigmohamed6004]

how to calculate the non-synonymous and synonymous substitution rates

[@sosa222222]

thanks

[@majidiyoussef2023]

Sequencage by hybridization

[@CristianoRaashid77]

Bro I want to fly to wherever you are and give you a hug

[@shomusbiologyofficial]

Thank you for appreciating my efforts

[@amiralialamdari590]

Greaaasaaaaaaaat😍

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@nazmulhaque4718]

u r best sir :3

[@sayamiri7574]

رائع 👍

[@bakeursaadaoui1251]

Merci