Comparative genomic hybridization

[@mahnoorkhan9784]

I owe my bachelors and masters degree to you sir

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@izekesama6820]

6 years later still saving lives

[@shomusbiologyofficial]

Thank you

[@subhamoydas6837]

Exacty.... 🤘Always shomu sir👌🤘

[@piotrkopytko6542]

9 years haha *

[@indian62353]

10 years later too

[@pratikshaantil9144]

Now 9 😂

[@kefeibao96]

When I try to find some video of teaching aCGH, your lecture showed up and it's so clearly to understand the basic principle and thoughts of it, so helpful! Thank you, friend.

[@shomusbiologyofficial]

Thank you

[@Sam-ie1qv]

I have genetic exam tomorrow and your videos make it so easy thank u from the bottom of my heart. God bless you

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@mahamijaz6001]

I can't believe that thus video is 10 years old. We both friends are getting your lectures and didn't even realise...very efficient and effective material..thank you ❤

[@shomusbiologyofficial]

All the best for future career

[@soumaizushiro1601]

literally 11 years later, and still does the job sir. hats off for your work.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@kashafseducationalsite363]

sir.. i m so so thankful.. u cant understand how i m feeling relief after watching this . u cleared my all confusion.. saty blessed .. thank u. from Pakistan

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@matthewmillett1225]

Great explanation Shomu. Thanks for all the great work you do! I love how you also explained balanced translocation almost as an afterthought in less than a minute haha.

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@raahul1303]

Sir you are simply the best. You beat all the lecturers I've had so far

[@lizbethshellysaldivar8390]

I was having a really difficult time understanding this concept from my readings, but this one video made me understand it perfectly clear. Thank you for the informative video!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@achigwecynthrus2343]

9years later and still the best video…. Thank you

[@shomusbiologyofficial]

You're welcome

[@darshinimalukar3246]

This guy and his teaching skills. So grateful (y) you help us alooooot of us. Thank you

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@fayzio5282]

Thank you so so much! I have a genetic's exam soon and understanding these methods was the hardest part for me! You saved me!!! *virtual thank you hug*

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@rimel6446]

7 years later and this is still helping us

[@KuldeepSingh-fu7gl]

You are a great teacher Shomu! Your explanation was very helpful in understanding the concept.

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@marwabelgh7403]

you are amazing you saved my life thanks a lot from Algeria

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@shalinividyadharan3763]

thank you sir for making the concept so simple

[@shomusbiologyofficial]

You're welcome

[@bersah4517]

Shomu u the man. Helping us for years

[@shomusbiologyofficial]

You're welcome

[@nayimmoissi1820]

im a french guy but you are explaining very well so i can understand everything you are saying, thank you !

[@elir.393]

This guy is my hero, best teacher.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Robertson284]

you surprised me with your explanation. thank you so much.

[@shomusbiologyofficial]

You're welcome. Glad you like it

[@solodesign]

Your explanation could not be better... Excellent sir!!

[@soumikdeb5436]

Very nice and clear teaching... Thank you

[@melvinmesekansalar2108]

Very well understood now, thanks Shomu!

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@selsabilfattouche4337]

Thank u sir after 7 years

[@Happy_Rocks]

Awesome illustration !! made it easy to understand

[@sivaji-xy7ez]

Excellent explanation 👍🏻👍🏻👌👌

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@zinhninhtet157]

Thank you for the video. Good explanation.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@Austin-xx4cs]

Thank you man .. appreciate the demonstration .. really made it easy for me

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@Ahsan___]

sir kal paper ha . paper sa phla apki video dekho everything clear

[@shomusbiologyofficial]

All the best

[@junaidminhas6984]

you are an amazing teacher !

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@manjuchauhan5280]

Too much helpful .ur all videos are the best 👍👍

[@shomusbiologyofficial]

+manju Chauhan thank you very much

[@DoctorKenji]

Made it so easy to understand, thank you!

[@aigbekaenraymond6049]

Really helpful. Many thanks

[@shomusbiologyofficial]

You're welcome

[@wafimalik7355]

thank you sir... u saved my live

[@shomusbiologyofficial]

You're welcome

[@mohammedabbaskhan2127]

TY Shomu Daa🙂

[@shomusbiologyofficial]

You're welcome

[@YourLocalIndianSamosa]

Very nice video, briefly and yet very nicely explaining the whole concept ...Thank you for uploading in such a video. I learned a lot from it !!!!

[@CopperKettle]

Thank you! Cheers from Yekaterinburg, Russia!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@malikayoussouf9406]

May Allah bless you. You have helped us so much, you put so much effort and make it so much more logical. Thank you so much.

[@sukh6566]

excellent video sir

[@shomusbiologyofficial]

Thank you

[@hafsakhalid8503]

Thank u..simple and crystal clear

[@shomusbiologyofficial]

You're welcome

[@ridwansyahfkuii]

nice explanation, thank you sir

[@shomusbiologyofficial]

You're welcome

[@nOnAmE-nl3qu]

Thank youuuu so much 🥺 you saved me 🙏

[@shomusbiologyofficial]

You're welcome

[@SuperYaniv12]

Awesome explanation! thank you very much sir!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@poyosis]

What's the difference between CGH and aCGH? Is it the same thing? And when do you use the BAC vector???

[@abhishekmukherji2332]

Great job boss 👍🏼

[@shomusbiologyofficial]

Thank you

[@m7amad2000]

That was a fantastic explanation. Thank you Sir!

[@jonasdekeyser4916]

Thanks from belguim really good illustrated

[@saroshkhan6259]

I'm passing this degree because of these lectures sir.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@luyingchen8454]

Thank you for the video. It is really clear and helpful. But I think there is a small problem concerning the ratio of blue: red fluorephore. First, if the blue:red = 0.75, then there is more red fluorephore, the color should be more red. But you used the blue pen to draw it.But maybe you didn't mean to correspond the pen color to the real color. Second, the second ratio of blue: red you wrote is 1:0. But I think it should be 1:1. Anyway, the video is very constructive.

[@Marco-jc3ny]

Helped me so much! Thank you so much!

[@drshefalinaag2701]

Nice ,informative video

[@shadinasser1]

Great explanation, thanks

[@markaszkenasy7395]

Nice clear explanation,thank you.

[@juliablaszczyk8036]

Thank you for the easy explanation of that method! :) I have only two questions: Should the value be not higher than 1,25 in case of duplication? And the same thing, should the value be not lower than 0,75 in deletion?

[@bluesofi]

Very helpful! I love your videos! Thank you so much!

[@ermanistifli8074]

Dear All There are two serious mistakes in this video! 1. In CGH experiments, we isolate the reference DNA from a healthy individual. But this guy argues that both the tumour and reference DNA are isolated from the same individual. This is a scientific flaw. 2. The tumour and reference DNAs are not hybridized to each other! We hybridize them on normal metaphase spreads! 3. The term that he uses "Metaphasic" does not exist. "Metaphase cells" is used instead. Please be aware that this video contains serious scientific flaw! But it is not surprising because everyone can upload videos on youtube.

[@zedor1553]

I believe that for clinical research, it is normal to extract DNA from tumor and compare this DNA with surrounding healthy tissue from the same patient. DNA from healthy tissue serves as control for studying new chromosomal abnormalities in tumor cells. I can't encounter the scientific flaw at this point, and I'd be really grateful if you could explain this point further. And, I would appreciate if you write your references if you claim that "everyone" can upload videos here. And, as well, anyone can comment on them. And "Metaphafic" is indeed an inaccurate term.

[@ermanistifli8074]

Dear Zhannie Could you please show me a study that utilizes the same individual as a source for both reference and test DNA?

[@93Royston]

Erman İstifli, Thank you pointing out the mistakes. but instead of simplying commenting down, you could tagged him in your comment. so that the mistakes could be brought to his notice.

[@nitkaigla]

Erman İstifli Good to know :) I had the same doubts watching this video

[@93Royston]

but sometimes cells from the same person are used.

[@nahlaomar9072]

😲😲😲Mann this guys teachin..CGH was always difficult for me until God showed me this Mann...PS: i hav completed my MD pathology

[@Michael-il8ls]

more advanced information would be useful. how do the data look like? what is resolution? how are data processed (examples of data files)? also how many fluoriscent probes are on each chromosome? how do mutated chromosomes hybridize each other? can they wrongly hybrydize? how many chromosomes are there from each cells? 1 or are they amplified?

[@pramitbanerjee]

the data is image files. You use certain software to quantify the fluorescence values, then you can use matlab to analyze them

[@testtest7727]

Great video. It was really helpful. Could you make a video about Copy Number Variation(CNV)?

[@Diwakarkumar-ms7op]

Thank u so much ❤

[@shomusbiologyofficial]

You're welcome

[@diogocosta7654]

I have a doubt...what about when we're talking of genomic diseases like for example cris du chat syndrome. From where do you take the 'normal' DNA? because all cells have this mutation. Thank you

[@tomasgybel1964]

I guess it is not appropriate diagnostic technique then :/ Maybe something like M-Banding, SKY or M-FISH is better for like "cursory diagnosis".

[@r3g4rds]

you can use an array. So you can use a-CGH.

[@zaramirza7745]

great video! keep up the good work!

[@lifeandbeyond7279]

hybridization is not between the test and reference genomes (DNA), but between the microarray oligonucleotides and the both test and reference genomes (DNA).

[@qwerty975311]

Helped me out! Thank you!

[@tarigmohamed6004]

how to calculate the non-synonymous and synonymous substitution rates

[@farisalshboul998]

very good illustration ,,, thanks , May Allah bless you :D

[@Sydneyinfoclips]

Hey bro may I know what's your qualifications? I have been following you since long and saw you explaining almost all aspect..

[@zohaibamjad153]

Thank you very much sir, brilliant video :)

[@kamleshprajapati1482]

Thanks a lot fr explanations.. helpful it is!

[@SpeedSai]

Thank you so muchh❤

[@shomusbiologyofficial]

You're welcome

[@이영선-v2r]

thank you so much! it's very helpful for a newbie like me!! :)

[@kardo9381]

Perfect, Thank you dude.

[@PoonamKaushik-z6h]

Hum hybridization of genomes m ye fluroscence kyu use krege??

[@Rjajdjdj]

Thank you sir... I understand very easily. 😊❤ Some other confuse me to acgh btw cgh

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@titusmatteo3925]

very schlau Shomu!!!!

[@MuradAhmadzada]

It was helpful, thank you!

[@FMob2010]

Thank you! That was very helpful! :)

[@fari1025]

thank you , very helpful

[@tanisarker1112]

thank u bro............. very helpfull

[@Aasterias]

Thank you so much!

[@zhuoyueyang7125]

Thank you

[@shomusbiologyofficial]

You're welcome

[@aqsarehman9877]

Ofcourse it's very helpful

[@shomusbiologyofficial]

You're welcome

[@ZakaToubi]

Thanks man keep on the good work ;)

[@majidiyoussef2023]

Sequencage by hybridization

[@sorooshghasemi7135]

helpful thanks for sharing your knowledge with us :))

[@shomusbiologyofficial]

You're welcome

[@miladibdah]

very helpful ! thank you !!

[@yossora3892]

🥺 well explianed

[@shomusbiologyofficial]

Thank you

[@deepthir1643]

Is this GISH?? Please reply

[@manjunebhani9671]

Very nice..

[@shomusbiologyofficial]

You're welcome

[@artbylupiv]

Loved it

[@shomusbiologyofficial]

Thank you

[@arunimachakraborty1450]

very helpful

[@poojasalodiya5100]

Thanks soo much sir

[@kennixcheung2270]

Fantastic!!!

[@amiralialamdari590]

Greaaasaaaaaaaat😍

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@JuhiMittal]

thank you :)

[@nazmulhaque4718]

u r best sir :3

[@CristianoRaashid77]

Bro I want to fly to wherever you are and give you a hug

[@shomusbiologyofficial]

Thank you for appreciating my efforts

[@bahahos7426]

i lovee u my brother tank you from DK

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@bahahos7426]

@@shomusbiologyofficial

[@sosa222222]

thanks