Gene mapping part 6 Fluorescent in situ hybridization

[@ayeshanadim6911]

Your explanation is very clear sir..... best teacher

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@ЛидияСамарина-ч5ъ]

Thank you Mr. Suman, your explanation is very clear.

[@jennyroy7431]

Thank u so much sir .... a big fan of ur vedioes and physical mapping in clear through this vedio

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@SaumyadipSarkar]

Very useful. Even I have future planing of doing FISH. I must say i have seen multiple videos this not at all fishy. Keep pouring your knowledge.

[@supriyaa2511]

Thank you so much sir Apna Vedio gulo sob somoy e khub helpful hoi💖🙏

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@rishita_sharma4805]

Thanks sir for making this video it's very helpful for me 😊

[@shomusbiologyofficial]

You're welcome

[@trivedi2784]

King of biology 💫👍

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@soumyaranjanmahanta9127]

Thank you so much 😊 sir 🙏 for these videos..And for making Gene Mapping crystal clear 🙏😊.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@khushboo-e-mausiqui8559]

Thankyou so much sir,it helped me a lot

[@shomusbiologyofficial]

You're welcome

[@saroshkhan6259]

Thank you so much sir. This is very very helpful.

[@shomusbiologyofficial]

You're welcome

[@clinicalworld5077]

A very nice video. Keep going and spread the knowledge 👍👍

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@HCLpradeep2010]

And I belive when you referring a segment here ..it means you referring the a gene in side that chromosome....bcl6 gene you targeting by bcl6 probe against chromosome 8 to find a pattern fusion or red or green color to conclude normal or abnormal at loci 8q24 for example...

[@ArpanHaldarFlute]

It's very helpful. Thank you

[@shomusbiologyofficial]

You're welcome

[@maitreyeehira3463]

You are the saviour...🙏 thank you very much

[@ervinchan9919]

hi great video, but i have a question here that needs further clarification, this FISH method is done inside the nucleus of a cell of interest and is viewed under microscope, my question is how do the designed probe attaches itself to the un-denatured DNA strands that are present inside the cell nucleus or that this FISH method targets mRNAs instead?

[@iconmir]

Actually the probes designed are specific against the gene of interest. Your question is how does the hybridization occur with in the nucleus as the reaction is in-situ. Apparently, the DNA of interest is denatured leaving the strand of interest partially apart and the designed fluroscent probes are incorporated and raised which are complementary to the DNA of interest and the intensity of flourescence is measured

[@saicharanvarma411]

Ervin Chan which DNA is taken for making probe?

[@arunkumarchopwad9915]

THANK U VERY MUCH SUMAN

[@uddhavkumawat1217]

happy b'day sir

[@arunkumarchopwad9915]

Uddhav Kumawat thank u

[@minalchaudhary1746]

But Sir How we know that probe is bind with our gene in which chromosome number(1,2,3,....

[@HCLpradeep2010]

Thank you so much dear..can you please give a session on FISH ISCN nomenclature ..I am not able to get a clear picture on the notations from various cases

[@hrkikram2089]

Thank u so much

[@shomusbiologyofficial]

You're welcome

[@jayhind-ln5se]

Awesome sir

[@shomusbiologyofficial]

You're welcome

[@alizatasnim4702]

Is the genetic material not lost when we are cutting the dna for insertion of neuclotides???

[@red-draken9880]

I must admit, your looks deceived me into thinking it will be one of those garbage videos, but boy was I wrong. Very well explained.

[@nemesisdeimos92]

That's pretty rude

[@prajaktadekate5022]

Very helpful... Thanks..🙂

[@langpuarti1389]

Thanks

[@razosman9581]

Thank u so much it's so great

[@nailaimtiaz3617]

What is denaturation temperature

[@nailaimtiaz3617]

I am confused you said dutp but RNA IS NOT deoxy only DNA IS DEOXY

[@parvathalumadgula3742]

Same doubt

[@firozsheikh3352]

great video👏👏👏

[@bhartiuppadhyay5234]

thnks😊

[@ZimbardoBandura]

At around 18:50 what do you mean by "plating chromosomes"??

[@Anietta777]

+Farouk Aizat "painting chromosomes" ! it's like a comparative molecular cytogenetics technique

[@kelius158]

graziah

[@alizatasnim4702]

What is genetic marker??

[@shomusbiologyofficial]

Specific genes

[@arunkumarchopwad9915]

It is now understood that I did not get that far