Coomassie Brilliant Blue Dye - the science of SDS-PAGE staining

[@mahdis.the.curious]

I love you for making this video. you're a true angel, please keep making these useful videos! thank you, thank you, thank youuuuu!!!!

[@thebumblingbiochemist]

Glad you found it helpful!

[@chen-hsuanchiu3265]

Very informative and I like the energy you share in the video!

[@thebumblingbiochemist]

Thank you!

[@muhammadkaleem4251]

Bravo. Super helpful. Thank you so much for making this video :) Thumbs UP :)

[@thebumblingbiochemist]

Glad to hear it - thanks!

[@muhammadkaleem4251]

I have a question: What if we destain too much and the bands are too faint to be visualized, can we restain the gel with Coomassie and then destain again?

[@thebumblingbiochemist]

Yes

[@MES-S]

Thank you for sharing a wonderful information

[@Darcy2531]

This was super helpful!! Can you tell why we take the ratio of absorbance 590/450 while performing Bradford assay?

[@thebumblingbiochemist]

Thanks! You take the ratio because it allows you to kinda normalize for background and make the standard curve linear: www.ncbi.nlm.nih.gov/pmc/articles/PMC3164080/

[@jandecoole5687]

very clear and correct explaination. thanks alot

[@thebumblingbiochemist]

Thanks! Glad to hear it

[@stefanodecler149]

Great explanation and so complete, thanks :) I have a question: How much time do you suggest to stain with R-250?

[@thebumblingbiochemist]

Thanks! Probably like 20 min or so - longer if you need more sensitivity but then the destain takes longer too! Microwaving can help

[@MarianaAguilarMorales]

can you share the volume of staining solution used and the time used at the microwave? thanks!

[@thebumblingbiochemist]

Just enough to cover the gel and 30s-1 min spurts usually

[@MarianaAguilarMorales]

@@thebumblingbiochemist thank you!

[@khoinguyenlehoang6000]

thank you very much!

[@thebumblingbiochemist]

You're very welcome!

[@qayyumshehzad3412]

very informative, thank u

[@thebumblingbiochemist]

Glad it was helpful!

[@morgul_r3420]

LMAO I love this intro. HAHAHA