Western Blot (WB) Visual Protocol

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In the western blot visual protocol video, you will learn how to prepare your samples before loading them into a gel, load a gel and separate the proteins through electrophoresis, transfer your proteins from the SDS-PAGE gel onto a PVDF or nitrocellulose membrane, block the membrane, stain in Ponceau red, add the primary and secondary antibodies, and visualize your protein of interest. Additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

[@MrWyrmlord]

This... this is an amazingly beautiful blot. Hard, black bars, no background noise, no unnecessary ladders. I think I'll just print it out and hang it on the whiteboard. You have my respect.

[@neurobiogirl]

THANK YOU!! Very clear, concise and articulate.

[@mohamedabdellatif1489]

Absolutely brilliant and very descriptive...thanks a million

[@ATARISING]

Thank you for the tutorial. It was very helpful and informative and has given me a better understanding of the protocol. I will be running my first ever Western Blot in the next few days!!

[@laylays_world16]

This is a great video. Very informative to collaborate with my presentation on western blotting. Thanks!!!!

[@khadijahm.m.7178]

Really thank you. This video is really easy to understand. Thank you again.

[@CR-uu1rr]

Very detailed clear exhaustive you have taken away all my doubts . Spectacular step by step. Amazing

[@zulkifleekuedo6177]

Thank you so much. It is very clear and helpful WB video for me

[@gaurangtrivedi3713]

Simply amazing..!! Brilliant

[@PatreeceCJYou]

Thanks for posting this. Helps me with my exam! :)

[@dragosgheorghe9350]

Outstanding. From someone who tested hiv negative using eclia and just found out if positive it is recommended to use this technique. I am stunned, amazing work

[@deekshamarwari7112]

Thank you so much for uploading this video 😊🙏

[@MonaraKaelle]

Great, thank you!

[@frass99]

A very beautiful blot.

[@mmst3620]

Very helpful tutorial video, thanks

[@seshadri16]

Very well explained, helped me understand the protocol much better !! Thanks for the video !!

[@NovusBiologicals]

Glad that it was helpful!

[@user-hk6qu7rq2n]

awesome. now im ready for my experiment next week

[@madeleinem9357]

Thank you. So amazing!!

[@NovusBiologicals]

Glad that you enjoyed it!

[@shahidkhanalvi9519]

Thank you 😊

[@nurture-nature...]

very well explained.... thanks a lot....

[@vidhyaanish6888]

the video was very helpful and explained each steps very clearly.Thanks a lot.

[@NovusBiologicals]

Glad that you enjoyed it!

[@BilalAhmed-vr8rq]

Very well explained

[@tilovatulkhondaker]

thank you, it was helpful

[@iranrose2002]

it was perfect i really appreciate

[@colombarillo9123]

Thank you!! Page bookmarked.

[@123superdeduper]

This is cool

[@reemalsereihi4705]

amazzzzzzzing

[@helmimuhammad7801]

GOOD

[@deekshamarwari7112]

We want more biological techniques vedioes in this manner.. please upload more videos of other techniques in detail 🎉😊🙏

[@hassoeai]

Great tutorial! I like the two chamber tray used in phase 3, membrane transfer. where did u get it from?

[@MA-dq9uz]

world

[@irenakavrakova3801]

when we incubate with more then 1 Antibody do we have to block for 1 hour before every incubation

[@MisterAlbertoPiano]

Great video! Amazingly helpful and very well explained. One little question: In this case, what was the final ECL volume used on the last incubation? I know that it depends on the surface of the membrane (e.g. 0,1mL ECL/cm^2 aprox.) In my case I have a 7 X 8.5 cm membrane, so I just need 6 mL of ECL (following the manufacturer's equation). The problem is that I'm not very sure if 6 mL would cover the membrane in that type of Incubating box that you use (mine is extremely similar). What would you do in my case? You would increase the volume of ECL even is not needed or you would incubate just with 6 mL ECL. Thank you again for uploading this great video. It help us out a lot!

[@NovusBiologicals]

Glad that you enjoyed the video! In regards to your question, to be safe use 10 ml of ECL, this is what use. Excess ECL is not a problem. The key is to make sure the surface of the membrane stays wet and does not dry out. You can add the membrane to the ECL and rock the chamber back and forth to keep the surface wet. Hope that helps!

[@MisterAlbertoPiano]

***** Thank you very much for your quick response!! It will help a lot!

[@nilsjohansson4222]

when and where do you put copper stain?

[@NovusBiologicals]

Hi Nils, thank you for your question. The Copper stain is a reversible stain that, while not required, is an optional step. The Copper stain is used on the gel itself after gel electrophoresis to confirm the migration and separation of proteins in the gel. This step would be performed prior to the membrane transfer and would require destaining the gel before proceeding with protein transfer. Hope that answers your question!

[@UNEARTHED36]

Premade gel!? I have to make mine.

[@bakhtawarkhan9547]

Thank you

[@francescogallo4562]

Bicocca gang

[@robertsullivan5450]

How i manage to do all that at full concentration working inside a lab which is monitored the whole time with a obnoxious lab lady?

[@robertsullivan5450]

a german lady to be more specific

[@TheRand20]

hmm why is she putting her hand outside the hood and then back again without cleaning them ? if everything must be sterile, you should never stick your hand outside the hood.