CRISPR Gene Editing: Using CRISPR-Cas9 with the Out of the Blue CRISPR Kit

[@Zane-It]

i hope this channel makes more videos on this topic

[@Meme-b2i7i]

Thank you for the clear explanation💯

[@BioRadEducation]

You're welcome! Be sure to check out all of the videos on our CRISPR playlist from Bio-Rad and other KZbin channels!

[@gaby31052000]

Brilliant video

[@prakashkannan7274]

Well explained thanks guys

[@marc8239]

Hey I’d like to make my pet hamster glow in the dark, can I get the kit?

[@TeslaWorkshop]

i want to bioengineer myself

[@BobbyMaler-fy6wh]

I want to make my arm twice as long so I can reach my glasses without getting out of bed. Any help?

[@lill_yblack4]

No problem sir, you first isolate bioluminescence from fireflies..then put in your cell it can make you glow in the dark...like your pet.

[@sprout4096]

@@BobbyMaler-fy6wh go buy some tongs

[@Shaq0112]

The best - thank you!

[@KrishnaKumar-gs5mg]

superb.

[@엄기원-f7v]

좋아요!

[@adeelmehmood7439]

Good

[@capgains]

How does this relate to cognitive abilities ?

[@misterhemp]

is there a continuation?

[@Karen-jj4fr]

Can I use this to knock out myostatin gene

[@BioRadEducation]

Hi, Karen! The kit only knocks out the lacZ gene in the bacterial chromosome. It is for educational use to teach CRISPR gene editing and isn’t able to be adapted to knock out other genes.

[@BioRadEducation]

Hi,@Pi Pony! A different guide RNA sequence would be needed to target a gene other than lacZ. The Out of the Blue CRISPR Kit only targets the bacterial lacZ gene, as the guide RNA sequence is for that of lacZ.

[@BioRadEducation]

Hi, @Pi Pony! Yes: each guide RNA is specific for the DNA sequence of the target. Thus, if you wanted to target a different gene you would use a different guide RNA, and that guide RNA would have a specific sequence that matches the desired target gene. You can watch and learn about this and how CRISPR-Cas9 gene editing works in our CRISPR Explained: Gene Editing History, Technology, and Applications video at kzbin.info/www/bejne/qn_SlHSZarl-rNk starting around 15min and 40seconds of that video.

[@BioRadEducation]

@Pi Pony The guide RNA sequences could in theory be synthesized in a lab. However, in this system the guide RNA is transcribed from the pLZDonorGuide plasmid.

[@BioRadEducation]

@Pi Pony Yes, we're here to help! Contact us at bio-rad.com/ExplorerHelp

[@HewanDemissie]

I live in Ethiopia. This kit will be used to teach students about the CRISPR/Cas9 gene-editing technology. Is it possible for me to obtain it? If so, from what source? Thank you for the interesting presentation.

[@BioRadEducation]

Hi, Hewan! Let's continue this conversation - please send us your request at bio-rad.com/ExplorerHelp

[@mateuszbylewski298]

👏

[@MT-jb3pw]

Out of the Blue CRISPR Kit and Genotyping Extension is not on your page???

[@BioRadEducation]

Hi, MT - you can learn more at www.bio-rad.com/outoftheblue

[@themaskedmandate3633]

I would like to become spider man now

[@menmyself-f9w]

is this pseudomonas aeruginosa?

[@neo-filthyfrank1347]

did you even watch the video

[@chitrajawalanj7756]

could you explain the results please?

[@BioRadEducation]

Hi, Chitraja! Check out the FREE student and instructor manuals at bio-rad.com/outoftheblue for additional information and help to understand the experiment.

[@chitrajawalanj7756]

@@BioRadEducation it is coming that this content is not available in my region.

[@BioRadEducation]

Hi, Chitraja! Please send an email to us at [e-mail] and we'll help you out. Thanks!

[@srinivass442]

Lol I guess two blue, one white, one dead

[@Heyvx01]

Please can u explain the results in video ​@@BioRadEducation

[@dianalancaster4968]

What does the heat shock step do?

[@BioRadEducation]

Hi, Diana! The heat shock is part of the bacterial transformation step used to incorporate a plasmid, either pLZDonor or pLZDonorGuide, into the cell. For more info, visit bio-rad.com/teachCRISPR

[@solcarmo]

What is in the transformation solution?

[@BioRadEducation]

Hi, Solange! Transformation Solution is a sterile CaCl2 solution. You can learn more about the bacterial transformation and the role of transformation solution from our family of pGLO bacterial transformation kits: visit bio-rad.com/pGLO and bio-rad.com/teachpGLO for more info. The manuals discuss the role of transformation solution in plasmid transformation which, for the Out of the Blue CRISPR Kit, is the method in which the plasmids pD and pDG are introduced into the cells.

[@Mr.Cheeseburger24]

I want to gene edit to make bomb ass espresso, can I have a kit? 😇😇