DAVID Analysis, Gene Functional Classification

Analysis of cluster of Affy genes against HG-U133A background. Stringency set and evaluation of ontologies

[@ihnefee]

Thank you! Very helpful!

[@anjalipatni2580]

Background means what exactly?

[@MikhailDP]

The background is the "universe" of all genes, from which a list of genes of interest was obtained. For microarrays, these are all genes on the array, for RNA-seq, these are all genes, often subsetted to protein-coding transcripts. See "14 - Functional enrichment analysis" section here mdozmorov.github.io/BIOS567.2017/syllabus/ for more details on the topic of enrichment.