Electrophoresis, Immunoelectrophoresis and Immunofixation

[@MedicosisPerfectionalis]

🦠Antibiotics Lectures: www.medicosisperfectionalis.com/ 📝 Download Notes: www.medicosisperfectionalis.com/

[@nystagmus]

I am a doctor and teacher at a medical school. Not sure if you are a student, resident, or attending physician. BUT I KNOW YOU ARE A GREAT TEACHER.

[@MedicosisPerfectionalis]

Recent graduate! Thanks a million, dear :)

[@nystagmus]

Medicosis Perfectionalis ‏شكرا

[@mbimbi6157]

My hero brother man you just saved this residents tones of reading

[@MedicosisPerfectionalis]

Thanks dear :)

[@annettesuterswiss]

Hi Bro, I am just at the same place 🩺

[@Dr.Sarcoidosis]

قسم بالله رهيب المقطع، ما تعرف قد ايش موضوع الParaprotien كان معقدني لاني مع الأسف ما لقيت /صدر يشرحح زي الناس كثر الله خيرك

[@MedicosisPerfectionalis]

🙏🙏🙏

[@ReEvolving]

1st year med student here and you are making life easier, so glad I found this channel !! Thank you :)

[@Mazzawak]

I can't believe I finally found a video that makes me understand this subject. You gonna make me ace my exams, Egyptian Sensei.

[@mohamedal-agamy3650]

Wow,I never saw amazing way of simple presentation like this.very excellent.

[@MedicosisPerfectionalis]

Thank you so much 😊

[@sajadesign6354]

I would like to say THANK YOU sooooo much MEDICOSIS ❤.. Yesterday I finished my final exam in Medicine and your videos help me to solve most of the questions in the exam 🌸🌠 .. THANKS A LOT 💕

[@MedicosisPerfectionalis]

My pleasure 😇 I am so happy to hear that!

[@millylovesyou7703]

I watched the entire hematology playlist. Amazing!!

[@MedicosisPerfectionalis]

Wow 🤩 Awesome 👏 Thank you so much 😊

[@ivo3185]

You have videos on everything. Saving my life. Thank you!!!

[@sonyalisa30]

Extremely helpful explaining that SIFE is the same as immunoelectrophoresis, just the information I was looking for.

[@MedicosisPerfectionalis]

Thank you 🙏

[@hengchenmed5652]

Never find as good explanation as you do in this presentation, thanks 10000times

[@ghitabencheikh2747]

THANK YOU IM A PHARMACY STUDENT AND YOU REALLY HELPED ME!!!

[@erayerge5233]

the best explanition about this topic ı have ever listened. thanks lot.

[@Im_Azin]

I love your hematology course the most^^ Thank you🧡

[@aizankhalid7188]

Monoclonal gamopathies Plasma cell dyscrasia B-lymphocytes- Inc prod of plasma - Inc of antibodies Electrophoresis

[@nadialachkar1816]

Such a relief to finally understand this ! Thank you so much

[@MedicosisPerfectionalis]

My pleasure!

[@salahmab1]

Excellent comprehensive explanation makes the science so easy

[@MedicosisPerfectionalis]

Awesome 👏 Thank you 😊

[@dahmanimohammed3417]

شكرا جزيلا ملخص نافع. معلم علم المناعة من الجزائر

[@paulnitz8368]

How do you know that the area under the spike isn't compromised of "normal" (in range) immunoglobulins? How do you differentiate between normal/healthy/in-range Ig's and the monoclonal type. Confusing.

[@SamikGhoshMusicals]

Hello, thank you for such a well narrated video. I have a doubt..When we do a Serum/urine protein electrophoresis for a patient, the M spike we obtain for gamma globulin, does that spike comprise of only intact immunoglobulins? Or does it quantitate both intact immunoglobulin + any free light/heavy chain because the latter are also gamma-globulin?

[@joaovitorpimentel2140]

What a great explication! Thank you.

[@DZ72ALG]

Hi, I want to ask when gamma globulin is 17.5 g/l (ref: 6-16 g/l) means a person has multiple myeloma?

[@MedicosisPerfectionalis]

Not necessarily! There are criteria to diagnose multiple myeloma. Please talk to a doctor with experience.

[@jamiedaeon5665]

Merci beaucoup, vous m'avez aidé à bien comprendre l'immunofixation 💚

[@margarita09223]

Great video however I got confused and could please please someone explain when immunofixation is carried out,is anti- A the antigen, right? So why does it say add antibodies to antigen ? Can someone clarify this one for me ??Isn't antibody binding to another antibody that is specific to it?

[@al-husseinabdelhafeez3406]

So, serum-wise, are there only two techniques (Serum electrophoresis and Serum Immunoelectrophoresis/Immunofixation?

[@al-husseinabdelhafeez3406]

I mean, immunofixation and immunoelectrophoresis are two terms of the same technique, used interchangeably

[@hayaalmly6083]

Thank you!!!! Your channel made life much easier.

[@pinkyrose2116]

Can you tell me if kappa and lambda levels ara high but ratio is normal and kidney disease what does that mean?

[@BeckJSenSt]

I believe that there is a slight difference between the protocol of immunofixation electrophoresis & immunoelectrophoresis. However, in this video, they are considered the same thing. Is that my misunderstanding?

[@jamieemcclary4874]

Can u pls make videos about immunohistochem, flowcytometry , cytogenetic studies & molecular markers Brief info about these tests

[@MedicosisPerfectionalis]

That’s a great idea...I will add them to my bucket list!

[@luciamartin6444]

Thank you SO much for your videos, your explanations are always clear!!

[@MedicosisPerfectionalis]

My pleasure! This is so kind of you:)

[@ciroesposito5395]

what's the different between the immunofixaction and immunosubtraction?

[@Massi_35]

How do we get that graph? whats the name of the process?

[@AhmedIbrahim-yb1os]

Thank you very very much for ur perfect information, u r the best ❤️

[@MedicosisPerfectionalis]

My pleasure 😇

[@julianfalciola5237]

I noticed that in the IFE Immunofixation test that IGE and IGD are not not tested, just IGG, IGA and IGD are tested only. If someone with a high Monoclonal IGE or IGD, this Immunofixation test would come back to the patient and Doctor stating no paraprotein found. Do all Immunofixation test for the the complete panel of Immunoglubins? or do they just test for IGG, IGA and IGM only?

[@mariab492]

yes we only got test for igG IgA and igM because high monoclonal igE or igD is extremely rare

[@aishasalah6135]

Is this video for biochemistry lecturer of *plasma protein and their electrophoresis???*

[@MedicosisPerfectionalis]

It’s for students.

[@aishasalah6135]

@@MedicosisPerfectionalis 😅😅 I didn't understand your answer...

[@Halllookooohlhh]

what is the iep for immunoglobulins

[@poojaawasthi9232]

Thanku so much for such an easy explanation 🙏

[@wiememo8648]

I can't be more impressed by you, Dr.Medicosis, I've been a fan of your work for more than 3 years and I'm sharing your videos everytime I get the chance to be honest. However I have one question: how come you know all these information and can explain so clearly? Did you see them in your clerkship or rotations or is only from understanding textbooks? I'm really curious. Either ways, I'm so amazed by the job. You're wonderful, keep it up and may you be rewarded in this life and the other!

[@MedicosisPerfectionalis]

Thank you so much 😊 I just read a lot, take notes, and try to connect the dots together!

[@christiechow7261]

What about Ig D and Ig E? we don't need to run on immunofixation?

[@keyjackson5302]

Can anyone speak to M spike in alpha 2 and beta regions?

[@refayat7777]

Very Well Explained

[@Txpride13]

This video is helpful. Can you help me? My gastroenterologist ordered SPEP due to high globulin levels. My gamma globs are high but result says no M protein present. I do not have any Ige, IGD etc data in my results. Does this mean I may have to wait for immunofixation portion of the test??

[@itsmustafa9366]

شكراً جزيلاً

[@MedicosisPerfectionalis]

You’re welcome

[@nooshindeilamani6370]

You are amazing 👏

[@RyanMTube]

My doctor has requested I get my Immunoglobulins and Serum Electrophoresis checked. Are these the same thing or are they 2 separate tests? I can’t seem to find the answer online. My immunoglobulin come back ‘normal’ but I haven’t had the serum one back. I wasn’t sure if they are separate or not?

[@srinivasaraosirasapalli5104]

excellent doctor

[@MedicosisPerfectionalis]

Thanks again!

[@M1234-t9g]

nice video sir, please make a video on (FISH) Fluorescence in situ hybridization.

[@MedicosisPerfectionalis]

That’s a good idea 💡

[@M1234-t9g]

@@MedicosisPerfectionalis please sir make as soon as possible this my request to you, do not forget.

[@RıdvanÖzkan-b1s]

Sir, my Total Protein value is 82. My serum albumin is always normal, but it is low on Electrophoresis. Which one should I trust? I also have iron deficiency. Beta 1 was 10.2, the upper limit was 4.8. Beta 2 zone is very good, moderate and also Age 28 male, IgA: 2.07. (0.7-4) - IgM: 0.64 (0.4-2.3) - IgG: 14.35 (7-16) and SPEP Gamma region: 19 (10.6-19.2) and " "No monoclonal bands were found in the immunofixations." Do I have a problem? Is Gamma at the upper limit and IgG near the upper limit? Moreover, there is a sFLC Total fee and there is no problem, sir, what could be the reason? Alpha 1 was also at the upper limit.

[@noor5647]

Great video. Thanks!

[@anthonyanuforo6089]

Thanks. This helped a lot

[@MedicosisPerfectionalis]

My pleasure 😇

[@trishw2934]

Hi, that was amazing. Do you have any videos on polyclonal hypergammaglobulinemia?

[@MedicosisPerfectionalis]

Thank you 🙏 Unfortunately, I don’t!

[@trishw2934]

@@MedicosisPerfectionalis Would you be willing to make one?

[@princess_katana]

Extremely helpful!! Thank you so much!!

[@MedicosisPerfectionalis]

You’re always welcome 🙏

[@sorlos]

THANK YOU!!!

[@MedicosisPerfectionalis]

You're welcome!!

[@k.f9096]

Thank you

[@MedicosisPerfectionalis]

My pleasure 😇

[@G.Bfit.93]

Very high TPO Moderately high Anti-CCP Low Gamma Globulin Low Kappa Light Chain High LH and Prolactin Thoughts?

[@manalanin9968]

You are excellent machaellah

[@MedicosisPerfectionalis]

Thank you!

[@falahhassan6610]

Thanks

[@MedicosisPerfectionalis]

My pleasure 😇

[@vasigligor6914]

Very helpfull

[@mustafaoliwy282]

I love this man

[@MedicosisPerfectionalis]

Thank you 🙏

[@amirasaied5755]

Hi, HRU? I ve checked ur list but I didn't find videos about hemapharesis(plasmapharesis and cytapharesis) And heamatopoietic stem cell transplant Thank you

[@MedicosisPerfectionalis]

I don’t have them yet...Do you want them?

[@amirasaied5755]

😊😊 Actually I used to hear ur videos, write with u and then study my notes, today I am trying to finish hematology but I didn't find these videos, I ll be happy if u explained them but I don't want to overload you. Thank you

[@MedicosisPerfectionalis]

When is your exam?

[@amirasaied5755]

@@MedicosisPerfectionalis Ist of Mai, pediatric MD But I still ve alot of branches to finish.

[@نقاءوردهالصادقه]

thanke you so much 🤨🌹🌹

[@MedicosisPerfectionalis]

My pleasure 😇

[@Hasan-qg8uh]

Great video!

[@MedicosisPerfectionalis]

Thanks bro!

[@gianinaelenna3779]

Thank you so much!!! 😊

[@MedicosisPerfectionalis]

My pleasure 😇

[@سرمد-ن4ع]

Electro Phoresis الكترو فوريسيس

[@jadec9144]

love your videos

[@MedicosisPerfectionalis]

Thanks 😍

[@phoebewang5245]

thank you so much for your sharing

[@MedicosisPerfectionalis]

My pleasure 😇

[@shkokurda2098]

amazing presentation.thank you very much

[@MedicosisPerfectionalis]

My pleasure 😇

[@luisadelacruz6431]

thank you so much

[@mdtanvirrahman5971]

Excellent bro ..thanks a lot

[@MedicosisPerfectionalis]

My pleasure 😇

[@hebamarwan7648]

Excellent

[@MedicosisPerfectionalis]

Thanks!

love ur videos !!

[@MedicosisPerfectionalis]

Thank you 🙏

[@guru5796]

I am not a medical student or in such field......i really wanna ask you something...i do not have any bone pain my cretanin levels are good...but i got a m band in gamma region with a concentration of .86g/dl ....i dont have any symptoms whatsoever...but i do have protein discharge in urine.....what do you think ..can it be myeloma? I would really appriciate your take ...my reports of bone marrow dont come till next week....so just wanted your view on this

[@MedicosisPerfectionalis]

Did you do extra tests to establish the type of the immunoglobuline (e.g. IgE, IgG, IgM,...etc)?! Also, how old are you? What’s the calcium level in the blood? How much protein is in the urine? What are the results of your complete blood count (CBC)?!

[@theofanoanagnostou8821]

pretty good!

[@ashishverma-mt1ys]

Good... Thnxs

[@MedicosisPerfectionalis]

You’re very welcome 😊

[@nuna391]

"Pay attention" woke me the hell up.

[@MedicosisPerfectionalis]

Haha 😂

[@e8sw]

thank you so much 😍👌🏻

[@MedicosisPerfectionalis]

My pleasure!

[@sofiaahmed3872]

يتعافى المرء من يخلص فاينل

[@MedicosisPerfectionalis]

Haha 😂 That’s wisdom

[@ricodsanchez6792]

Nice vid sir

[@MedicosisPerfectionalis]

Thanks a ton!

[@beyazitdurdu3367]

top man

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Thumbs up.

[@nkworld7658]

🥇🥇🥇🥇🥇🥇🥇🥇🥇🥇🥇

[@dustinkover8756]

10/10

[@MedicosisPerfectionalis]

👍👍

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Wew....

[@MedicosisPerfectionalis]

👍

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Thank you

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Thanks

[@MedicosisPerfectionalis]

My pleasure 😇

[@saddamhossain2076]

Excellent

[@hishamabu1170]

Excellent

[@MedicosisPerfectionalis]

Thank you 🙏