Erik Jorgensen (U. Utah / HHMI) 2: Recycling synaptic vesicles: Ultrafast endocytosis

[@camilocastelblanco2903]

Fantastic talk! Thanks Dr. Jorgensen!

[@nirjharaloy6108]

Thanks Dr. Jorgensen! This is amazing! The selection between slow and ultra fast recycling wasn't clear through our conventional lectures.

[@Natedogie]

Thanks for the great lecture

[@chimerathirteen7057]

could it just be that ultrafast endocytosis doesn't occur at low temperatures and the only way to create synaptic vesicles is from clathrin mediated endocytosis since there is a lack of endocytic vesicles in the cell?

[@fips001]

isn't that exactly what he is saying?

[@chimerathirteen7057]

fips001 no, his hypothesis is that clathrin mediated endocytosis occurs when the neuron is over stimulated. Overstimulation is the stress condition he stated, not cold stress.

[@fips001]

both, look at 27:00

[@chimerathirteen7057]

Well not exactly. Yes he is stating that ultrafast endocytosis doesn't occur at the low temperatures. He then goes on to hypothesize why he doesn't see clathrin mediated endocytosis at 34C and hypothesizes that it will occur during overstimulation. It was the latter point I was addressing, not the 'how' but the 'why'. My bad, I guess I wasn't being too clear in my statement - I think the hypothesis can be generalized. It seems to me that under any condition where ultrafast endocytosis doesn't occur, clathrin mediated endocytosis would take over. Why is simple - there aren't any endosomes containing neurotransmitters in the cytosol for the clathrin to take apart and regenerate the synaptic vesicle pool. In this situation the only thing clathrin would recognize instead of the endosome surface is the cytosolic face of the plasma membrane at the active zone, which would have a similar composition to the endosome surface.