ESTIMATION OF HEMOGLOBIN - SAHLI'S ACID-HEMATIN METHOD | HAEMATOLOGY LAB | PHYSIOLOGY

Estimation of Hemoglobin
- Sahli's Acid-Hematin Method
- Haematology Practical
- Physiology and Pathology
- Sahli's hemoglobinometer Instruments
- Procedure
- Viva questions and Answers
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#hematology #physiology #hemoglobin

[@drhardikmistry]

HEMOGLOBIN THEORY LECTURE kzbin.info/www/bejne/e163kKGnr5d6nc0

[@rabbit5802]

Thank you.

[@firojabanumansuri9743]

Sir , the best thing about your video is the questions / answers which are usually asked in viva .💚.....thank u sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@panda-pw6eg]

Yes sir

[@sanem_khan]

Thank you so much. It was so helpful Ans very easy!!! Tmrw is my practical and you literally saved my time!!

[@drhardikmistry]

Thanks for your positive words. Please keep sharing.

[@rummufirdous9705]

Thank you so much sir I have my sem practical exam in few days and due to covid did not have chance to perform every practical practically this vedio made my understand visually and made my concept clear .Require more such vedios sir

[@drhardikmistry]

Best luck 👍🏻 Please keep sharing.

[@rujulbhavsar8745]

Thank you very much sir....once I had missed my clg hematology practical but after watching your video, I could understand the whole practical. Thank you very much for your efforts...

[@drhardikmistry]

Thanks. Please keep sharing.

[@rujulbhavsar8745]

@@drhardikmistry sure sir...🤗🤗

[@suvethaduraisamy3821]

@@drhardikmistry which type of hb can be estimated by sahli method sir?

[@medicKarim]

Sir congratulations to you for making this type of work well explained. I'm a Bangladeshi MBBS student. Love your dedication. 🎉 🎁

[@drhardikmistry]

Thanks. Please keep sharing.

[@prity4521]

Tomorrow is my practical exam thanks for this video I am searching this kind of video in which I understood everything Thanks once again sir 😊

[@drhardikmistry]

Best luck! Thanks. Please keep sharing.

[@syednashra.__]

Ughhh.. Finally found the video that i wanted.. Here procedure given is so easy to understand and write down on practical notebook... Tysm

[@31nikitakhairnar83]

Thanku sir🙏 ...I am frist year D. Farmacy student ..sir the best explained about ur this video is Q& Ans ..which are useful asked in viva 😇

[@drhardikmistry]

Thanks for your positive words. Please keep sharing.

[@nirbhaykumarsingh]

I am first year vetenary student Sir, Your video is very helpful for my LAB.

[@drhardikmistry]

Thanks. Please keep sharing.

[@swagatadas2557]

Sir your teaching process is so amazing. I want to be like you. You are my inspiration of medical field. Much love for you ❤❤.

[@bshashidhar354]

I absent the this experiment in college .now I understand this by seeing video . thanks sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@rukhsana2018]

FEMALES- 12-15gm/dL, MALES- 14-17 gm/dL, INFANTS- 16-18gm/dL, NEWBORN- 20-24gm/dL

[@yesharaj5611]

Thank you so much sir . You covered everything and questions answers too. Thank you so much again.

[@drhardikmistry]

Thanks. Please keep sharing.

[@candy13x]

The video is very informative... Most importantly it contains questions that maybe asked in viva Thankyou so much sir ✨

[@drhardikmistry]

Thanks. Please keep sharing.

[@nishitachaudhary9025]

Well done hardik thanks for providing us good material for achieving grt knowledge regarding practicals which are not doing by colleges ... thank you ❤️

[@drhardikmistry]

Thanks. Please keep sharing.

[@ritumattu4428]

Thanku so much sir. Great job. Having gud knowledge.. Great 👍

[@geekymedico.8099]

Thank u for making practical vedios sir... It's very helpful...🙏🙏🙂🙂🙂

[@drhardikmistry]

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[@rukhsana2018]

Some more VIVA QNS OF Hb RELATED TO BOTH THEORY and PRACTICAL POINT OF VIEW-- I STUDIED THIS SO JUST THOUGHT OF SHARING WITH YOU GUYS. 1. fxns of HB? TRANSPORT OF OXYGEN AND CO2 , BUFFERING ACTION, ALSO STORES IRON 2. Hb is tetramer or dimer or monomer? 3. Hb contains how many heme groups? 4. 1 Hb can bind how many molecules of oxygen? 5. Adult Hb contains 2 alpha and 2 beta chains. 6. Components of HB-- 4 heme groups and 1 globin protein. 7. Why the oxygen dossoc curve is sigmoidal? -- explain about how binding of oxygen takes place with Hb by theory of cooperativity. 8. some hemoglobinopathies names you should know.-- methHbemia, sickle cell anemia , and thalassemia 9. what is heme? It is an iron containing pigment 10. where is Hb present? RBC OF THE BLOOD 11. hemoglobinuria? presence of hb in urine. 12. STR OF HB? each hb molecule consists of 4 heme groups and one globin protein molecule-- one heme molecule consists of one protoporphyrin ring and an iron molecule and the globin consists of four polypeptide chains (2pairs). 13. SYN OF HB? you should have a basic knowledge -- hb syn starts in PROERYTHROBLASTS. HEME is syn in cytosol and mitochondria and globin syn in ribosomes. (full pathway you must know for theory exams) 14. how protoporphyrin fromed? 4 pyrrole rings joined together to form one protoporphyrin rings. 15. TYPES OF HB IS MUST. TYPES OF HB IS BECZ OF? it is beacuse of differnet globin chains. (the heme group remains the same) . 16. ADULT HB (HbA)- 2alpha and 2 beta chains 17. FETAL HB (HbF) - 2 alpha and 2 gamma chains. 18. what is oxygen dissoc curve show? it shows % stauration of hb with partial pressure of oxygen. 19. what is p50? it is the partial pressure of oxygen at which 50% of oxygen binding sites are saturated. 20. SICKLE CELL ANEMIA-- point mutation, autosomal recessive, at 6th position of the beta globin chain of the Hb. glu changes to valine -- which results is sickle shaped rbcs-- which reduces the life span of the rbcs-- and thus causes destruction of rbcs and hence -- hemolytic anemia. 21- METHhemoglobinemia-- ferrous iron converted into ferric iron-- methhb cant bind oxygen-- results in chocolate cyanosis-- that is brownish discoloration of the skin and the muscous membranes. 22- Thalassemia - autosomal recessive, the defect is in the production of the globin chains-- either the alpha or the beta chains are not syn-- if alpha not syn then known as alpha thalassemia and if beta not syn then known as beta thalassemia.

[@user-hs1du1wo3e]

Excellent demonstration thanx alot. Pro.Dr.SABAH AZIZ J. AL-OBOSI. IRAQ.BAGHDAD

[@drhardikmistry]

Thank you sir. It's a big complement for me. Please share with your groups and related circles.

[@deepaksingh-ut7lh]

Vividly explained ❤

[@prathikshanandhini1559]

Thank you for this video!!! really helpful

[@drhardikmistry]

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[@snehab6927]

Finally understood the practical

[@drhardikmistry]

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[@ganiyatmusa3225]

Thanks so much sir your lecture is easy to get

[@drhardikmistry]

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[@Anjali-xe5uc]

​@@drhardikmistrysir when I bow off the blood in tube it didn't go down I tried several times ... Is it because of clotting?

[@springsofsoul3560]

Excellent sir. thank you so much!

[@drhardikmistry]

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[@sumayatahira7819]

Best..our beloved teacher

[@drhardikmistry]

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[@alonymachor8163]

wow! u did a great job. thank you for the work done

[@drhardikmistry]

HEMOGLOBIN THEORY LECTURE kzbin.info/www/bejne/e163kKGnr5d6nc0

[@arindam_08]

Very good explanation Thanks 😘

[@prangyapritilipsapatra2593]

Sir plz uploading all methods.. Very nicely prepared this🤩

[@drhardikmistry]

For full hematology playlist Please visit: kzbin.info/aero/PLcVgXSU9gf3vXvZnT6w9Iw3p7kKCL5Ymj Thanks. Please keep sharing.

[@bhargavisanapala8031]

Please upload a video on jugular venous pressure examination

[@pragyabharti5447]

Very very nicely explained sir..best vdo ever..thanku sir 😀😀😀😀👍🏻👍🏻👍🏻👍🏻👍🏻👍🏻🔥🔥🔥🔥🔥✨✨✨

[@drhardikmistry]

Thanks. Please keep sharing.

[@vineethavini7990]

Helpfully video I like it 👍🏻

[@drhardikmistry]

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[@architadutta7344]

Thank you so much for this!

[@drhardikmistry]

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[@dishapatel7342]

Great explanation 🙂

[@drhardikmistry]

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[@HannahsHaven84]

Sir excellent class ❤

[@drhardikmistry]

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[@vijaypratapchoudhary763]

Very well explained 👏 👌

[@drhardikmistry]

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[@shinykj8536]

Thank you sir for the detailed explanation

[@drhardikmistry]

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[@merinapanyang3150]

Best video ever

[@drhardikmistry]

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[@nightrider-qf5uw]

Thanks such an nice explanation ❤️❤️❤️👍🙏

[@drhardikmistry]

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[@shivanishivani4705]

well explained 👏

[@pritamjitoinam8639]

Is the result obtain by sahli's method accurate? Or does the result obtain has some variation?

[@drhardikmistry]

No, it shows aproximate values. Thanks. Please keep sharing.

[@aminadilawar2969]

Sir this is the best vedio. Everything isdescrib

[@drhardikmistry]

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[@mdrummanuzzaman]

Best Covering all aspects

[@drhardikmistry]

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[@nayirarao5503]

Tqq very much sir this is veeeeery useful video

[@drhardikmistry]

Thanks. Please keep sharing.

[@aneeqahmad9100]

Can we use any other acid other than HCL for the estimation of hb? Why?

[@uniquewhatsappstatus7527]

thank you sir. detailed explanation....

[@drhardikmistry]

Thanks. Please keep sharing.

[@supriyabhagat3161]

Sir while checking the color of the solution in comparator box should we keep the stirrer inside the solution or outside?

[@drhardikmistry]

Stirer should be above the solution but must be present inside the hb tube. Thanks. Please keep sharing.

[@athena777_]

You are amazing ✨✨✨.

[@drhardikmistry]

Thanks. Please keep sharing.

[@AhmedKhaled-ou3dq]

Very good, doctor

[@drhardikmistry]

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[@aryakanudawala4847]

Thank You Sir!!

[@drhardikmistry]

Thanks. Please keep sharing.

[@akhilam626]

Very clearly explained

[@drhardikmistry]

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[@AB27editz]

Thanku Sir .Really helpful .❤️

[@drhardikmistry]

Thanks. Please keep sharing.

[@Vd_prabhat]

Thanku sirji💞

[@drhardikmistry]

Thanks. Please keep sharing.

[@trueline5563]

Thank u so much sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@hazel8537]

After putting hcl in the tube, should we mix the sucking blood sir

[@karthikeyavanimireddy3693]

Thank you so much sir 😌💖 💖

[@drhardikmistry]

Thanks. Please keep sharing.

[@ahmedrohy6469]

Thank you sir ☺️.❤️

[@drhardikmistry]

Thanks. Please keep sharing.

[@bookyaravi6289]

Good explanation

[@drhardikmistry]

Thanks for being here. Please share these videos with your groups.

[@Aiimsonian13]

Thankyou sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@kirubadevi2582]

Sir why the stirrer should not be placed opposite side inverted

[@tharanginipeetlatharangini7821]

Tq so much sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@bshashidhar354]

Super sir

[@aalokshah6207]

Sir it’s colorimeteric comparision or calorimetric?

[@akankshapriya2070]

Best video💕

[@rajasekar4239]

We have very useful video sir and again thank you so much sir

[@user-ze6gs5zo3u]

It really helpful 🙏

[@drhardikmistry]

Thanks. Please keep sharing.

[@surbhiv2024]

Vry nice sir 😊

[@drhardikmistry]

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[@ramyaadevi6036]

Well explained

[@drhardikmistry]

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[@user-ze6gs5zo3u]

Thank you sir 👌

[@hetvishah6641]

So much thank you💕

[@drhardikmistry]

Thanks. Please keep sharing.

[@riarathore274]

Thankyou Nice explanation

[@nibedita7445]

So helpful sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@jadumonigogoi403]

What if you take N/10hcl upto 10 mark instead of 20 ?

[@ishika_wanders]

Thankyouuu

[@drhardikmistry]

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[@patelvitixa656]

Very good

[@drhardikmistry]

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[@rebeccahmar1688]

It's very special

[@khushiarora8865]

Big fan of your's sir 🙏

[@drhardikmistry]

Thanks a lot. Please keep sharing.

[@aestheticqueen461]

Thank you so much

[@drhardikmistry]

Thanks. Please keep sharing.

[@akankshapriya2070]

Sir why in infants hemoglobin level is high?

[@Asquare369]

Awesome

[@drhardikmistry]

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[@prathameshgole2145]

Very descriptive video. Thank you sir

[@drhardikmistry]

Thanks for being here. Please share the knowledge with your groups.

[@arashdeepkour3361]

Awsmmm thank u 💯

[@30_gayatrigadariya35]

Thank you ! 😊👍🏻🙏🏻

[@drhardikmistry]

Thanks for being here. Share with your groups.

[@manuranjankumar5756]

Hii sir amazing lacture. Sir. Next video is how protin measured in urine?¿?

[@drhardikmistry]

Urine protein analysis is the topic of Biochemistry. Thanks. Please keep sharing.

[@rajasekar4239]

You do nice explanation

[@indianfashion2010]

Thanku sir it's too much helpfully for me

[@drhardikmistry]

Thanks. Please keep sharing.

[@nirupamroymajumdar2733]

Sir on the haemoglobin tube what does the "percentage" (not Gram percentage) denote? Today I performed this test.gm percentage value was 9.6 and percentage was 65%.

[@drhardikmistry]

Percentage is oxygen saturation value. Thanks. Please keep sharing.

[@Edutainment369]

Excellent

[@drhardikmistry]

Thanks for being here. Share videos with your groups.

[@Anagha2498]

Thanks sir 🤗

[@MedTechTalk]

Sir can you please make it clear that , in principle the comparison is calorimetric or colourimetic, it’s confusing me

[@drhardikmistry]

Colorimetric

[@MedTechTalk]

@@drhardikmistry thanks 👍

[@mazharmhd1416]

Thank u sir. Very nice presentation

[@drhardikmistry]

Thanks for being here. Please share the knowledge with your groups.

[@akankshapriya2070]

Why hemoglobin level is more in infants ..plsss answer,,, tomorrow is my exam?? I didn't get satisfied answwr anywhere

[@jismiabraham558]

Excellent sir..🙏🙏🙏🙏Thanks alot

[@drhardikmistry]

Thanks.

[@drstrange6706]

Nice video bro

[@drhardikmistry]

Thanks for being here. Share with your groups.

[@bravehearts9663]

Its really helpful.. thanks alot

[@drhardikmistry]

Thanks for being here. Please share the knowledge with your groups.

[@arpanbora8423]

Sir...this normal values are in accordance with which book??

[@Abijith..]

Thankyou sir Very well defined

[@drhardikmistry]

Thanks for being here. Please share the knowledge with your groups.

[@aryamalu4767]

Thqq sir

[@drhardikmistry]

Thanks. Please keep sharing.

[@dr.kuldeepojhavlog6514]

Nice sir👌

[@smilygrl2545]

Kown kown merejaise practical exam ke pehle din ye vdo dekh raha he

[@mayurgarg7626]

Sir it's humble request pls 🙏 upload video on ESR