Haemoglobin Estimation- Sahli's Method (हीमोग्लोबिन टेस्ट) By Solution Pharmacy

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Haemoglobin is the major component of blood which carries oxygen to the tissue for various function. when that goes down below the normal level one feels lethargy and fatigue. it's always better to estimate the haemoglobin level in blood.
After watching this video you will be able to understand- what is haemoglobin test, haemoglobin experiment, haemoglobin in Hindi, Sahli's hemoglobinometer, how to check haemoglobin in blood, haemoglobin estimation test, find your haemoglobin count, haemoglobin level in man, haemoglobin level in the woman,
Haemoglobin can be estimated by two methods-
Sahli's Haemoglobinometer and haemoglobin colour scale.
Step by steps procedure to estimate haemoglobin in blood by Sahli's method.
1- Take out the Haemoglobinometer and arrange its component in a stable place.
2.Prepare 0.1N HCl
3.Fill the 0.1 N into Sahli Haemometer tube up to 2% mark
4. Now place the pipette in Sahli Haemometer at a given place (in between two windows)
5. Now sterilize the fingertip and prick it with the help of sterilized lancet and fill the blood into Sahli Haemometer pipette up to 20 microliter marks.
6.Now transfer that blood sample into Sahli Haemometer tube in which we have already taken 0.1 N HCl.
7.Wait for about 10 minutes to let the reaction occur.
8.Now slowly-slowly dropwise add distilled water to its side and shake well with the help of given glass rod.
9.Keep adding distilled water till the colour of blood match the side window
10. Observe and note down the reading, that’s the percentage of haemoglobin
हेमोग्लोबिन रक्त का प्रमुख घटक है जो विभिन्न कार्यों के लिए ऊतक को ऑक्सीजन लेता है। जब यह सामान्य स्तर से नीचे चला जाता है तो कोई सुस्त और थकान महसूस करता है। रक्त में हीमोग्लोबिन स्तर का अनुमान लगाने के लिए हमेशा बेहतर होता है।
इस वीडियो को देखने के बाद आप समझ पाएंगे- हेमोग्लोबिन परीक्षण, हेमोग्लोबिन प्रयोग, हिंदी में हीमोग्लोबिन, सहली के हीमोग्लोबिनोमीटर, रक्त में हीमोग्लोबिन की जांच कैसे करें, हीमोग्लोबिन अनुमान परीक्षण, अपने हीमोग्लोबिन गिनती, हेमोग्लोबिन स्तर में आदमी, हीमोग्लोबिन स्तर औरत,
हेमोग्लोबिन का अनुमान दो तरीकों से किया जा सकता है-
सहली के हीमोग्लोबिनोमीटर और हीमोग्लोबिन रंग पैमाने।
साहली की विधि द्वारा रक्त में हीमोग्लोबिन का अनुमान लगाने के लिए कदम प्रक्रियाओं के चरण।
1- हेमोग्लोबिनोमीटर निकालें और इसके घटक को स्थिर स्थान पर व्यवस्थित करें।
2. 0.1 एन एचसीएल तैयार करें
3. 0.1 एन को सहली हैमोमीटर ट्यूब में 2% तक भरें
4. अब दिए गए स्थान पर सहली हैमोमीटर में पिपेट रखें (दो खिड़कियों के बीच में)
5. अब उंगलियों को निर्जलित करें और नसबंदी वाले लेंस की मदद से इसे छेड़छाड़ करें और रक्त को 20 माइक्रोलिटर अंकों तक सहली हैमोमीटर पिपेट में भरें।
6. अब हस्तांतरण करें कि सहली हैमोमीटर ट्यूब में रक्त नमूना जिसमें हम पहले ही 0.1 एन एचसीएल ले चुके हैं।
7. प्रतिक्रिया होने के लिए लगभग 10 मिनट के लिए प्रतीक्षा करें।
8. धीरे धीरे धीरे-धीरे ड्रॉपवाइड अपनी तरफ आसुत पानी जोड़ें और दी गई ग्लास रॉड की मदद से अच्छी तरह से हिलाएं।
9. खून के रंग से तरफ खिड़की से मेल खाने तक आसुत पानी डालना रखें
10. पढ़ना और पढ़ना नोट करें, यह हीमोग्लोबिन का प्रतिशत है

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[@asrithasrirekha4954]

The explanation is good... But we need to wait for atleast 10min after adding blood to n/10hcl .. For formation of acid haematin

[@solutionpharmacy]

Yes you are right. I forgot to tell this point . Thank you

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[@rajat4520]

Just a note: The stirrer should be lifted when comparing the colors, but not completely taken out of the tube. It's so as to avoid any potential loss of fluid which would lead to a lower Hb estimate.

[@solutionpharmacy]

Yes you are right. We did a slight mistake here. Sorry for that

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[@hindikaumparast6715]

For God's and your own sake, learn the correct method from somewhere else.

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After mixing blood with acid ,,wait for 10min and then add distilled water drop by drop

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nice ans.

[@yogesh3296]

The finger should not be squeezed to take out the blood, otherwise tissue fluid might come out,thus giving the lower estimation of haemoglobin

[@Joeythegoats]

Yeah but most of the cases we have to squeeze the finger to get enough blood at 1 time otherwise bubble will produce which will largely effect it and the blood in capillary tube may clot in no time. When we performed in lab most of students had to squeeze it or have to prick at different place on finger to get enough blood. With a tiny prick

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Not squeezing but can gently press

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[@arbulnisar8326]

Very helpful information. But u have not mentioned after placing blood in N/10 HCl in sahlis tube wait for 10 minutes And also In no case we don't have to take the stirrer out, add distilled water but don't take the stirrer out as it will contaminate the solution.

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Yes you are correct. Sorry for these

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Dude, who's gonna wait for 5 mins after adding 20 cu mm blood in dilution tube filled 0.1 with hcl for the formation of acid hematin.

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Take 5 to 10 minats observe time than add drop by drop D. Water

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swdesh sharma Exactly. We kept that for abt 8 mints but forgot to inform. Thanks

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Thank you. All the best.

[@nirupamroymajumdar2733]

Sir on the haemoglobin tube what does percentage (not Gram percentage ) denote? Today I performed this test. Gram percentage value was 9.6 and percentage value was 65%

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Thanks for explaining good explanation

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Some laboratories multiply the meter reading with 8 and 9 to get the result in decimals i,e:- Meter reading.....13 13×8= 10.4 gms% I still have a doubt

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[@doczeelkodia]

I obviously dnt knw better than u sir But it’s not advisable to remove the stirrer everytime Coz the blood loss occurs with the stirrer..! P.S pls dnt get offended sir Bt i just shared wt i knew :)

[@solutionpharmacy]

Zeel Kodia Your point is right. But we have already mixed the blood with DW so the blood volume is sufficiently maked up and it will affect the final result. Although your suggestion is highly acceptable and we are thankful for this.

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All the best

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Thank you

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Tq so much...

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shreya bl Its my pleasure madam. Thanks for your comment. Please take a look at our facebook page for more videos and study materials facebook.com/pharmavideo/

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Thank you Sir.

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Farheen Qureshi my pleasure

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All of my teachers

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You are most welcome sir. It's my pleasure that I could clear your confusion. please keep supporting solution.

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[@solutionpharmacy]

Great. Thank you so much for your feedback

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shouldn't we wait for 8-10 minutes after adding blood in hcl ?

[@solutionpharmacy]

Yes we should wait for few mints.

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yes correct...!!!!

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Write

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Something 10 minutes

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Thank you

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tyx sir

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DNYANESHWAR KADAM welcome sir

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Awesome video sir..sir, but distilled water humne side wall se ku add kiya centr me se ku nhi??

[@solutionpharmacy]

Slowly jaye isilye

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While mixing never take the glass rod out 😐😐....

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mera 20 min baad

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@@liasiid 30 mins🙂

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15 min

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Sab wahi kar rahe 😂😂

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Bohot acha hai sir 😊

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Thank you so much

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DMLT wale like karo

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Thnxx sir for dmlt students May Allah bless you 🤗

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Kon Kon practical hone K 15 min phle Dekh rha h mere trh🙋🤭

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Hume ye experiment na to btaya na he krwaya fir bhi dekhna pad rha h

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Aj maine b kiya but ye video dekh ke clear ho gayA

[@solutionpharmacy]

Priya Mehra thank you for your feedback

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Waching this video just before 1hour of viva 😅😅and never done this practical in lab. #backbencher 😂😂

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You try to give the video more brightness it will be great if you do

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Very well explained sir

[@solutionpharmacy]

Thank you