Facile preparation of a self healing hydrogel

[@markwyn2040]

Thank You for sharing your scientific method!

[@justinjohnson2510]

How would I make this adhesive for a sucking chest wound seal? I want to make my own chest wound seals. This is perfect, so how would I make it adhesive?

[@sasarello8124]

Dear Justin, I strongly do not recommend any medical applications. These experiments are for hobby fun only. Best regards

[@anitarani3419]

I'm doing research on Collagen based hydrogel. I watch your vedios and got impressed by your work very much.Dear I request you to follow this article method (novel collagen based hydrogels with injectable, self healing, wound healing properties via dynamic crosslinking interaction) to make a video for me. Because I don't know how to synthesize the hydrogel of this article.

[@sidraarshad-l1f]

I follow your method and made the gel...but its not adhesive......

[@artbygunnu]

Why bubbles formed,is there any reason

[@Whitedeath63]

Thank you for this wonderful video. I have a question. Could we incorporate graphene or reduced graphene oxide to make it conduct?

[@sasarello8124]

I think that graphene/graphene oxide can be incorporated but I don't know if it will be conductive.

[@ichrakalbouchi3256]

Thanks for this great video, could it word with alginate sodium cross linked by calcium chloride?

[@sasarello8124]

I think I would not be self-healing.

[@l-o-o-h]

Wow, this is fascinating! If I heat up the hydrogel, would it become viscous again?

[@sasarello8124]

Sure, it will recover the viscosity

[@l-o-o-h]

@@sasarello8124 Could this be made with Xantham gum as well? Or any other gum?

[@sasarello8124]

@@l-o-o-h In my opinion not. this is because the reaction with borate requires two hydroxyls close together as in guar gum. I am not aware of any other polysaccharides with such characteristics. Best regards.

[@l-o-o-h]

@@sasarello8124 you’re amazing, thank you!

[@BravoSixBC]

Thank you for this information. Would it be possible to add Activated Carbon so it becomes more conductive to ions? What about adding NaOH to make it more active for batteries ? Thank you in advance . Kind Regards

[@sasarello8124]

Dear Bravo, I think it is an interesting idea. My concern is regards the guar gum long term stability in contact with NaOH; but you can try. Best regards

[@BravoSixBC]

@@sasarello8124 thank you for your reply. Yes, indeed your video is an excellent achievement , no doubt about it. I don't know if the NaOH would destroy the healing stability . I will try definitely . Do you know how long your gel remains hydrated ? Thank you in advance .

[@sasarello8124]

@@BravoSixBC I have samples stable after 6 months. Borate is an antimicrobial agent. Best.

[@makayultia828]

I am wondering, is there a way to make the crosslinking process slower so that I can remove the bubbles inside of the hydrogel? I would like to use an empty glass bell

[@sasarello8124]

Just a crazy idea: add more glycerol in the recipe, this should slow down the speed of crosslinking or inhibit it. Then, whasing the hydrogel in water the glycerol could be extracted. I can not guarantee that this will work. Best

[@WorthyBuzz]

If you mix using an Ultrasonic Emulsifier, you should be able to dispense with most of the air bubbles

[@HritankhiTripathy]

If we keep it in water, will that get dissolved?

[@sasarello8124]

Hi, No it will not dissolve because the gel is crosslinked

[@alaaalahammadi6795]

thanks for sharing ,, bu I got a question ,, can I use pectin powder instead of guar gum ??? also can I use CaCl2 insted of Borax as cross linker ?

[@sasarello8124]

I did not tried with pectin but I think that with pectin the reaction does not work. Calciun would work as ionic crosslinker but the reaction with borax is totally different.

[@alaaalahammadi6795]

@@sasarello8124 do you have an email or any thing i can connect with you by , cuz I am doing my graduation project and all my attempts just went wrong , i have a problem i cant find ,

[@sasarello8124]

@@alaaalahammadi6795 [e-mail]

[@mithiltrivedi5204]

When I try to washing it with distilled water for convert crude gel to pure gel but from this way hydrogel is fully spread in water. Also water distributed the shape of Gel it mean it convert in viscous solution…there is any other way to washing Hydrogel???

[@sasarello8124]

In my opinion the chitosan should be washed when it is in base form (it should not gel), after the hydrolysis with NaOH. Then, after acidification it will became a gel and adding water it will disperse and viscosizing the solution.

[@makayultia828]

I am a bit confused (and I'm sorry about it); how does the vitamin B12 get involved?

[@sasarello8124]

Vitamin B12 was used just to color the gel. Regards

[@makayultia828]

Thank you for answering!@@sasarello8124

[@makayultia828]

Thank you very much for answering! ​@@sasarello8124

[@Chembatt_IITR]

During preparation of GG hydrogel i have used 2m ZnSo4 salt solution instead of water. can you please tell me what will be the effect of this? Also can you please highlight the reason for the formation of bubble in the hydrogel. Thanks in advance

[@artbygunnu]

Same doubt plz anyone answer

[@JeffYuYaoChiun]

For the artificial skin example, can you please provide the volume of water you used?

[@sasarello8124]

Dear jeff, the recipe was: 58 g of a 1.5% Guar gum and 5% glycerin solution mixed with 7.5 ml of a 4% borax solution. If we consider approximately the density of such solutions equal to 1, 65.5 ml (58+7.5) of final material will contain 1.33% guar, 4.43% glycerin, 0.46% borax and so 100-1.33-4.43-0.46= 93.75 ml of water.

[@JeffYuYaoChiun]

I am specifically referring to 4:05 in the video, when you mentioned decreasing the water content for a denser hydrogel. Thus instead of 1.5% guar gum, what was the higher concentration?

[@sasarello8124]

Dear Jeff, honestry the samples was left to dry at room temperture and so it lost moisture. Unfortunately I can't retrieve the exact number. Two suggestions: 1) prepare the 1.5% guar gum gel as in the video and let it to loose moisture (room T or into a ventilated oven at 40°C) or 2) start directly with an higer concentration of guar gum (e.g. 2%) but in this case will be difficult to prepare a gel with high concentration of polymer. I would prefer the first method 1)@@JeffYuYaoChiun

[@JeffYuYaoChiun]

@@sasarello8124 Thanks kindly. This is good guidance and I'll experiment a bit.

[@chrisplaymusic]

Correct me if i am wrong but this type of hydrogel can also be used as a scaffold for cell seeding right?

[@sasarello8124]

I think yes. Moreover the borate into the gel may work as preservative

[@ibragim68]

Hello! Please tell me what it is used for? And can it be used for cosmetic purposes, for example, to create patches? Thanks)

[@sasarello8124]

Hello, to my knowledge it is used mainly as a toy for kids. In my opinion it could be used to create patches. Regards

[@ibragim68]

@@sasarello8124 Oh, thanks) I'll do it soon

[@likithj638]

I tried to prepare the same above as you mentioned in the video, but I didn't get thick gel as you got. What should I do??

[@sasarello8124]

Hi, This is very strange because gelling of guar gum with borate is (generally) very easy. Basically you need: guar gum, borax, water and glycerol. May I ask you which kind of reagents did you use? Best regards, Alessandro

[@likithj638]

@@sasarello8124 the same as you used

[@likithj638]

I did more trials, but the result was same. Also are the quantities which you mentioned are exact or rough??

[@sasarello8124]

@@likithj638 I looked at the video: in fact there can be some confusion. As regards the preparation of the gel, 0.45 g of guar are used on a total volume of 50 g, so the concentration is about 0.90%. But when crosslinking I used a 1.5% guar solution so to prepare 58 g as in the video you have to use the following recipe: 0.87 g guar + 2.9 g glycerin to 58 g with water. Then crosslink by adding 7.5 ml of a 4% borax solution. Let me know if this finally works!

[@TensinghJoshua]

Hi sir, when should we use cyanocobalamine? I don't know if I am missing something.

[@sasarello8124]

Dear Joshua, Yes you could use cyanocobalamine. Best R

[@WorthyBuzz]

The B12 you ask about was only used as food coloring to demo the mixing taking place during self healing of the product

[@manalichakraborty5175]

Can we make a hydrogel by using chitin by this same method?

[@sasarello8124]

Sorry for the late reply. Unfortunately I think not, chitin si not soluble and I think it should not react with borate ions

[@kartik3528]

does 4% borax mean wt/wt and how much time before you do cross linking

[@sasarello8124]

Yes, 4% means wt/wt. Regards time before reaction, I would say that is very fast; it depends on the concentration of the borate solution but I would say it is a matter of 10-30 seconds.

[@AutomaticFlax4470]

Is it possible to synthesis a version of this that is hyperthin, perhaps thinner than a millimeter?

[@sasarello8124]

I think yes. You can try depositing a thin layer on a glass slide and wait, the nature of the gel will cause it to flow and decrease in thickness. Alternatively, you can deposit a more diluted solution and wait for the excess water to evaporate by decreasing the thickness

[@herrkommandank675]

Hi, I'm planning to manufacture my own non-toxic and ideally natural hydrogel, guar gum, glycerin and the deionized water are alright, but I'm worried about the borax solution, where the FDA considers it a toxic chemical agent and could cause unwanted symptoms. 1. Is Borax Solution safe for medical application for wounds and for medicine in general? 2. Is Borax Solution really needed? 3. Is there an non-toxic or natural alternative to borax solution? 4. What study did you base this on? Thanks for your response.

[@sasarello8124]

Dear kommandank, Borate has a long history of safe practice into consumer products, (e.g. detergents). However, it is under investigation by authorities since a lot of years. Unfortunately there are not, in my knowlede, substitutes that works in the same way for guar gum. I would suggest you to try another gel system based on sodium alginate crosslinked with calcium ions. Best regards.

[@herrkommandank675]

@@sasarello8124 Thank you for your response!

[@artbykshama8482]

@@sasarello8124 sir what is the sources of this formulation?

[@sasarello8124]

@@artbykshama8482 In the video I cited an article; however, the reaction between guar gum and borate is very well known since long time. I hope this helps

[@zainabjawad122]

Can i use myrrah in stead of guar gum? Myrrah also belong to the family of gums. And can i use this hydrogel for hard tissues regeneration, Such as bone grafting applications?

[@sasarello8124]

Dear Zainab, first of all sorry for the late reply. Going to your question: I do not think that myrrah could be used instead of guar gum. However, you can try....

[@duytai852]

Cool!!! Can i use it for my video about self-healing hydrogels?

[@sasarello8124]

Yes, go ahead!

[@duytai852]

@@sasarello8124 thanks sir...

[@aaradhyborode5576]

Can I add encapsulated essential oils in the formulations?n in what conc.?

[@sasarello8124]

I think yes. I would start with a 10% of capsules. Regards

[@behnampn9908]

can you explain how to make it injectable?

[@sasarello8124]

I hope this answer is not too simplistic: in the indicated proportion the gel can be aspirated and dispensed from a syringe. Regarding why this happens, I believe that the nature of these gels is very sensitive to shear stresses: if not subjected to shear they can appear solid but once subjected to a shear force their viscosity decreases. For example, sand mixed with water in a container can be solid but if you start vibrating it becomes liquid.

[@kartik3528]

Is 7.5 ml tap water

[@sasarello8124]

No, in each case I used demineralized water. Best Regards

[@kartik3528]

@@sasarello8124 so i made it using demi water itself but mine turned out a bit on liquidish side i think the linking wasnt done properly i added 5ml of borax more than required so now i am going to make it again what precautions should i take this time on

[@kartik3528]

@@sasarello8124 Thanks for all your replies, I am really thankful to you.

[@christianoberlin1996]

U rock 🪨

[@meysamkordi4944]

Goodbye 👋