FIJI (ImageJ): Semi-Auto Tracing & Analysis of 3D Microscopy Images with BigTrace
Рет қаралды 1,319
Күн бұрын
@Pat_Y87 7 ай бұрын
Johanna I really want to thank you for this! It took me months to figure out this problem with 3D tracing, and I was so relieved this plugin worked out! Thank you so so much, and keep up the great work! You’re the best
@johanna.m.dela-cruz 7 ай бұрын
I’m happy this worked out for you. Thanks for your support.
@Pat_Y87 7 ай бұрын
@@johanna.m.dela-cruz Hi quick question, what is the difference when we render our image in max intensity vs volumetric? Because my image seemed to be more blurry when I render them in volumetric
@johanna.m.dela-cruz 7 ай бұрын
@@Pat_Y87. In Maximim intensity, the voxels with maximum intensity are projected in the visualization plane. Volumetric corresponds to "transparency rendering" or "alpha-blending" ray-casting. Which is better? It depends on your dataset really. You can tweak volumetric rendering so that it looks better by using the opacity slider ("α range") slider in the Brightness and color dialog.
@Pat_Y87 7 ай бұрын
@@johanna.m.dela-cruz I see! Thank you again for the help!
Johanna M. Dela Cruz
Рет қаралды 3,8 М.