FIJI (ImageJ): Splitting Connected Objects with Watershed

[@electronmicroscopist1986]

Great comparison of the many threshold methods in Fiji! Thanks! 🙂

[@johanna.m.dela-cruz]

@electronmicroscopist1986, Thanks for supporting my channel.

[@宋放]

Thank you for this excellent tutorial, it's really helpful.

[@johanna.m.dela-cruz]

Thanks for watching. 😊

[@FaridDE]

Hey! Great video. I'm completely new to fiji and was wondering : is it possible to convert czi files into mp4 or any other standard video format?

[@johanna.m.dela-cruz]

Hi @FaridDE. You can save your .czi files as .AVI.

[@FaridDE]

@@johanna.m.dela-cruz of course wow thank you. My laptop was taking too much time opeining and rendering 234 frames - thanks for the help again.

[@FaridDE]

@@johanna.m.dela-cruz I have another question : I'm using carl zeiss zencore and axiocam color 305. How can I go about recording a 24 hours stream without taking a ton of space on my SSD (zencore generates .czi files that are huge, like 6 gigs for 30 seconds files).

[@jamilaiqbal202]

Hi johana. Will you be able to make a tutorial on TrajClassifier and TrackAnalyzer? Thanks

[@johanna.m.dela-cruz]

Hi Jamila. I haven’t used those plugins, but I’ll look into it. Thanks for your suggestion.

[@FaridDE]

Hi again ! I want to export an .obj (wavefront) file from the 3d interactive surface plot. Is it possible? because when I went the normal way (file>save as) it resulted in a 2d file, not a rotatable obj file which can be imported to powerpoint for instance.

[@johanna.m.dela-cruz]

Hi Farid. Unfortunately, the interactive 3D surface plot plugin does not offer any other way to save the surface plot.You could try using 3D Viewer, and display the image as a surface plot, then create the animation for rotation.

[@FaridDE]

@@johanna.m.dela-cruz Thanks for your reply. Indeed Image J is unable to directly generate wavefront obj / stl files from the interactive 3d surface plot. Another solution I found is Microsoft 3D Builder (blender is a hassle lol). Thanks again !