Flow cytometry : basic principles | What the use of flow cytometry ? | Cell sorting by FACS

[@TheLionheart94]

Currently applying for an acting senior role in an immunology laboratory as a biochemist. I haven't studied flow cytometry for many years and it is a core area that the role looks after, therefore this video has been a brilliant starting point to try and get my head around things so thank you very much! :-)

[@animatedbiologywitharpan]

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[@angie565656]

I am French and I understood everything, thank you ! I’m applying to a job and I have an interview tomorrow (I have never done cytometry before 😰)

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All the best for your job interview

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lol same

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[@danaab9735]

That's an excellent and a very simplified and straight to the point clarification! Thanks a lot! I love your channel.

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[@H_golds]

Thank you thank you thank you for this series. This would have taken me hours to understand using google. Your videos are so easy to follow and concise, explaining the basics of a complicated technique in simple terms, and your visuals/diagrams are fantastic. Cannot thank you enough, you might have just saved my grade.

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[@josephdahdouh2725]

That was so clear thank you. I watched several on this topic, and could only now really understand where that graph comes from and how the pressure, flow, light, size, and complexity all somehow play a role in the final result of the diagram and the separate forward and side diagrams. Thanks, didn't expect I would understand it this well...

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@@animatedbiologywitharpan I wish I could, but I come from a broke ass country, and I am almost broke... Me leaving a comment will give this video slightly more traction and will indirectly help this video get more views.

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[@earthandmedicine3667]

Thank you! This was very helpful for my pathology rotation as a 4th year medical student.

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[@zaphodflys]

Very clear explanation with great minimalistic diagrammatic explanation! Thank you!!

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Glad you found it helpful! Please share this with your friends or classmates.

[@heleniroanya]

The way you explained this oh my gosh, I cannot thank you enough!

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[@brianayee884]

Very much appreciated this video. Can't wait for more!

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[@brianayee884]

@@animatedbiologywitharpan Will do!

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@@brianayee884 Thanks a lot

[@roshnibhatt2801]

Thanks!

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your support is our motivation. Thanks a lot

[@phoebejuma6954]

Wonderful. Makes it so much more clear!

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thank you! this was a very nice intro before going to class

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[@yasasiperera2948]

Hi I'm from Sri Lanka. Thank you for the very clear and comprehensive explanation.

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[@ramenscreams231]

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[@Harold_Ofori]

Very clear and straight forward about FC basics. Thank you

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[@fatima8305]

Thank you so much! Your effort is appreciated.

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[@commongrade89]

Great explanation! And it only took mere minutes. You are a wonderful teacher👍

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[@truc1201]

I started my day watching your video (I am going to a course of Cytometry today but it will be in French, which will be complicated for me). In short, you saved my life😂 Thank you so much

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Thanks for the compliment. It makes my day nicer.

[@rezkyilhamsaputra8472]

Thanks a lot, it really helped me understand the concept in just a few minutes

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[@antoniotommasopinto3462]

thanks so much, this is a very clear summary of flowcytometry

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[@bardacha100]

Man, many many thks for deep work and clear presentation!

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Thank you!! This helped me so much for my exams. Keep it up ❣️

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[@wbgw6476]

This was a WONDERFUL video! Thank you very much!! :)

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[@candiceord1590]

Great simple explanation, thank you!

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[@sunlydia7718]

So a nice video, very clear.It's a good beginning for my immunology lab

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[@drio9589]

Thank you sir! This is a very good, clear, and concise video.

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[@manjildiganta9556]

how do you read graphs that have 4 quadrants, and then in the x axis you have FITC and in y axix you have APC. thanks

[@meghasharma2505]

So wonderful explained! Thanku so much!

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[@thunderstorminmyblood3705]

super helpful and clearly explained, thank you!

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[@dmoots2663]

this was so so so helpful, thank you!

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[@abhyarthanabehera4802]

Wow. Thanks again for giving me a better idea. 👍

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[@tripathyseema]

Thank you . It's very helpful and easy to understand the concept .

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thank you so much for the explanation, this has been incredibly helpful! x

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[@BernardCouvreur]

very complete and clear !

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[@ceylan4948]

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Very clear introduction. Thank you

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[@mr.lonely.S.T.]

Thank you so much！🙆‍♂️This is really helpful！

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Well presented. Thank you.

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[@natalias374]

thank you very helpful for chemical biology mid term

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[@mariatso3889]

thank you, this was so easy to understand keep up the good work!

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Thank you very much for uploading this :)

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[@tinepone]

Phenomenal .. I understood so fast!

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[@kiaekim3466]

Thank you very much! It is so helpful

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[@ushanikaushalya3044]

Thank you for your amazing explanation🥰

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[@EthanRiver]

It’s very clear, helps me a lot!!!👍

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Very helpful, thank you!

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Best explanation 👍👏👌

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[@rivasarkar]

Thank you. Helped a lot

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Thanks for this!

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[@anyoon8829]

The side detector seems to focus on how long the cell is. The forward detector can only detect how wide the cell is. Gravity factor comes into play on this machine

[@SofiaColledan]

thank youuuu awesome and clear!!

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[@davideschiliro]

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[@tayyabmohammad3845]

Nicely done sir . Appreciated

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TRULY HELPFUL!

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Again Sir you won heart, Thank you very much 🙏

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@@animatedbiologywitharpan Sure Sir; Sir, Mahendra Sonawane Sir is in TIFR now?

[@milica9136]

Hello! Thank you for this information! Its easy understandable! I have a few questions. What are the odds of flow cytometry? How likely is that device, that is based on flow cytometry, counts cells wrong? For example, to count lymphocytes into monocytes? In what cases device might be wrong (I am asking only in context of flow cytometry)? Could you direct me to the sources where I can read more about these questions? Thank you very much! :)

[@animatedbiologywitharpan]

If parameters are not set well it will show wrong things

[@nikitalokhande4063]

Thank you. Good explanation & presentation

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amazing explanation!

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Very helpful 💯👍🏻

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[@amanuelbekuma8373]

Good work!

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excellent video

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[@ahmedsadique9916]

Really good content

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[@shubhanginisrivastava672]

Great work!! you explained it very well and made it look easy👏👏 Also i cant find ur next video about FACS, can you pls provide link of FACS video?

[@tejaskhire]

I was also looking for FACS video. Can I get the link? Thanks !

[@koespiana]

thank you so much for such a good explaination!

[@animatedbiologywitharpan]

You're very welcome!

[@aishwaryamhatre5032]

Thank you so much sir 🙏❤️

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

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Excellent straight-forward video! Thank you!

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[@Nature-Walk-With-Me]

Excellent video

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[@simmywong3538]

Thank you so much for the incredible video!! :) May I know why PBMC instead of whole blood is used in flow cytometry?

[@paramitadas1756]

thank you soo soo soo much bro ..you are really great ..grateful to you

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[@snehalshende-z4h]

Hello sir..... by that graph we can understand which one lymphocytes and which one is monocyte or neutrophile????? Plz ans my question

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Yes it is possible

[@laurenwalker7165]

Great video

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Thank you so much!

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Thank you for your explained

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Thank u for great explanation

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Very nice video

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[@fallseason5163]

Perfect 👍🏻

[@QueenBee-kh7bx]

thank you so much! very nice video

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Thank you 🙏

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[@tripathyseema]

Thank you sir .

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Thank you so much Hare Kṛṣṇa 🙏😇

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[@skyminds9192]

simply super.

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[@libra1807]

very useful. thanks 👌

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[@abdallahouchene2697]

I love your vid thank you

[@animatedbiologywitharpan]

Thanks for watching!

[@stardreamix786]

Thank you so so much :)

[@animatedbiologywitharpan]

You're welcome!

[@fallseason5163]

Would you please help in analyzing data resulted from flow cytometry in some examples?!?!

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[@magicalcosmos]

very nice ...keep it up

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[@magicalcosmos]

@@animatedbiologywitharpan sure 😇

[@shailvishrivastava4403]

Thanks a lot sir

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[@gloriasolomon6165]

Wowww thank you so much

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Thanks a lot 😭🫶🏻🤍🤍🤍

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[@shilpanaik8930]

Hlo sir, I have one dut... Why flowcytometry will give different colours?

[@animatedbiologywitharpan]

Good question Shilpa. Fluorescence activated cell sorting (FACS) of live cells separates a population of cells into sub-populations based on fluorescent labeling. Sorting involves more complex mechanisms in the flow cytometer than a non-sorting analysis. Cells stained using fluorophore-conjugated antibodies can be separated from one another depending on which fluorophore they have been stained with. For example, a cell expressing one cell marker may be detected using an FITC-conjugated antibody that recognizes the marker, and another cell type expressing a different marker could be detected using a PE-conjugated antibody specific for that marker

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Do you have lecture notes for this?

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Thank you so much it's great

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Helpful

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Great stuff thank you.

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Thank you so much

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