Forensic DNA Profiling, Part I

More info and downloads: dnalc.cshl.edu/resources/dnal...
Although the DNA from different individuals is more alike than different, there are many regions of human chromosomes that exhibit a great deal of diversity. Such variable sequences are termed “polymorphic” (meaning many forms) and provide the basis for forensic identification. This experiment uses gel electrophoresis and a DNA chip to examine a highly variable locus within the human genome, like the ones used by the FBI for DNA fingerprinting. This locus, D1S80, contains a variable number of tandem repeats, which can be used to build an individual's unique DNA profile. In this first part of the experiment, join DNALC educator Jeffry Petracca as he demonstrates how to extract and purify DNA from cheek cells using saline mouthwash, and amplify the D1S80 locus by PCR.
Presenter: Jeffry Petracca
Audience: High School, Undergraduate

[@DNALearningCenter]

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[@robinburk166]

It's amazing how you seem to have a script inside your head and remember every word of it. Very well done, I'm a bit jealous of your teaching skills 👌

[@priyadarshini5477]

very good effort to explain VNTR and its role. we need many more videos of DNA fingerprinting

[@patipami78]

This video was very helpful. Thanks

[@priyadarshini5477]

excellent job

[@AbdulBari-yz9fg]

Excellent presentation that help me a lot in preparing my exam .Thank you for such an informative video

[@masnitamasri]

Tqvm, i've been looking 4 this kind of explanation for quite some time. Your explanation is technically very detail. Lov it. 👍👍👍♥️🤩🤩🤩

[@ashamerine6958]

At 14.03, its possible to get unique repeats for a loci, so its not necessary that STR profile of an individual should be an 100% matched to parents?

[@VinodSingh-ol2jh]

Awesome sir

[@shriyamehta617]

Have you use any restriction enzymes here? Or just DNAses to fragment DNA?

[@rubyvanveen3002]

No, for a pcr you don't use restriction enzymes

[@robinburk166]

The DNAses are actually kept INactive due to the removal of the calcium and magnesium ions. It is plain genomic DNA, of which a part, defined by the primers, will be heavily amplified in the pcr reaction.

[@Nathelie]

You talk too fast)