Ghetto Serial Spore Dilution and Plating (from swab) for Monokaryons

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[@xj11w2c7]

You are doing cool stuff man

[@notecarton9832]

I want my getto Ed1k PhD!

[@bradleybryan1970]

Loving all the content you're putting out!

[@junerobinson1220]

How many mL of water can one use for serial dilution regardless of bottle/tube size. Can it be whatever ? As the main goal is to dilute and isolate ? Appreciate you

[@edwardgrand]

I find using a total volume of 10 mL for each dilution convenient. Start off with 10 mL, add/dilute/shake, then use 1 mL of that to the next container with 9 mL of new water. Do that 3 times and then you will have your 1X, 10X, 100X, 1000X. I plate out 1 mL of each of those. Then you get a wide range of germination rates to choose from.

[@junerobinson1220]

@@edwardgrand I’m going to assume this can be done with centrifuge tubes as well? That’s what I have. Very thorough response. Nuff Respect my brother 🙏.

[@AdmiralAckbar666]

Love you Edward🤘😉

[@gangsterbuilder]

you make it so easy. im learning

[@Robebabu]

I just noticed all your videos are flipped. Everything reads backwards.

[@AverI_IrevA]

where do you get these cute glass bottles

[@edwardgrand]

I reuse little sports drink bottles.

[@brucetheshroom9973]

What type of agar recipe are you using? Will the agar absorb all that water???

[@edwardgrand]

Standard MEYA. It will absorb that water in 30 minutes.

[@dustinewing6767]

You put the wrong cap on you -2 vial I'm pretty sure brother

[@edwardgrand]

Yep. Wasn't worth making the video again. Doesn't matter.

[@unknowni1190]

So do these methods to get a diko an mono an then would you take agar transfer an put both in a dish to grow out an hope it's a successful mush cross?

[@unknowni1190]

Sorry I'm jus now trying to learn the right way to breed/cross genetics I only know the ghetto swab tek cross

[@edwardgrand]

@@unknowni1190 This is the method to get monokaryons for mating with other monos or di-mon crosses.

[@unknowni1190]

@@edwardgrand oh OK yea I'm still learning about crossing. So is there a difference to cross mono x mono or mono x dimon

[@unknowni1190]

@@edwardgrand you have vid on the ghetto swab cross? Where you swab the fruit of genetics with same side qtip

[@edwardgrand]

@@unknowni1190 No. You described what you do. One swab, two fruit. Put it to agar. I never use that technique, but I'm sure someone else has a video on it.

[@Robebabu]

9:25 different cap

[@dustinewing6767]

I saw it to bub