Graphical Estimation of Kd from P:L Binding Plot

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[@santanamendoza4279]

I'm very surprised that this video doesn't have more views! By far the best explanation of these graphs I have seen thus far. Thank you!!!

[@tendekaim]

omg you save lives! you have no idea...shortest and best explanation ever thank you

[@Silent_Sounds]

you are so much better at explaining these concepts than my tenured professor at a nationally recognized research institution. Props and thanks for helping me pass biochem.

[@h8charvs]

Brilliant video, thanks!

[@kalkidanberhanu7708]

This is just amazing! Thanks :)

[@muhammadkhattak6922]

Thanks a lot for the video, you are great at it.This helped me very much with my school stuff. Keep up the good work.

[@umamaheshwari1878]

+Catalyst University good

[@AmitKSingh-pk3ll]

very nice explanation

[@ching-hung-yao]

very good video help me a lot

[@fondue542]

Thank you!!

[@atawahperry1852]

thanks was useful

[@curtis4994]

you're fantastic

[@SohanurRahman09]

Nice video...Thank you very much.

[@CatalystUniversity]

Thank you!

[@shushmagudla3920]

So are Km and Kd similar? Km also substrate concentration at 0.5 Vmax. How are the two different?

[@thatboyss5576]

Would this not give the initial concentration instead of Kd. We would then use this initial concentration to solve for Kd using the equilibrium equation.

[@emilyh6442]

So a higher Ka would also mean tighter binding/higher affinity? Thank you

[@animeandstuff5377]

no problem

[@ummeamara5942]

how to get y-axis value?

[@busoladaramola9616]

why cant it be at 100% theta, completely saturated ?

[@robertluxenhofer1579]

Not using 100% theta has a simple practical reason. As the curve inevitable slowly, asymptotically approaches 100% the 100% is hard to determine experimentally. The 50% value, however, is much more simple to determine.