Homology-Directed Repair: How the Cell Edits DNA After a CRISPR-Induced Break

Sometimes DNA breaks because of insults like x-rays, UV rays, or genetic scissors (e.g., CRISPR-Cas9). DNA breakage can have serious consequences if not corrected. Luckily, the cell has mechanisms to repair those breaks, including one known as homology-directed repair. In this fun animated clip, scientists describe how homology-directed repair works in an accessible way. You’re left with a haiku to better remember it.
This clip was taken from HUMAN NATURE, our documentary which explores the discovery and future implications of the CRISPR-Cas9 genome editing technology.
#HDR #HomologyDirectedRepair #CRISPR #DNARepair #RepairMechanism #DNABreaks #DoubleStrandBreaks #CRISPRCas9Mechanism #FyodorUrnov #GeneEditing #HumanNatureDocumentary
Related Resources:
Human Nature, the documentary: www.humannature...
CRISPR 101: Homology Directed Repair (Addgene): blog.addgene.o...
Credits:
This clip was taken from the feature-length documentary Human Nature. Find credit info at: www.humannature...
© 2023 Science Communication Lab™. All rights reserved.

[@kotosqoposrly]

The quality of this video is ridiculously amazing!

[@shannaw7865]

I am a biotechnology student. I came here to figure out what is the difference between non-homologous end joining and Homology directed repair, but never expected to end up watching such an amazing explanation and video about this. Really appreciated it. Marvelous and astounding video !

[@scicommlab]

Welcome and thanks for watching!

[@monakiani191]

exactly same here :)

[@bobu5213]

This video is so perfect.

[@scicommlab]

Thank you. We are glad you like it.

[@js.4383]

Amazing video! loving the samurai cutting the DNA on the t-shirt :D

[@yaseminylmaz6291]

How you fool the cell?

[@gregorysmith1134]

With the donor repair template, the repair is made via hydrogen bonding before the cell's natural repair mechanisms can spring into action.

[@kedarnavsariwala348]

why tf did I understand HR better in this video rather than reading an entire research paper on it

[@biotechstories]

If we are capable of modifying the sister DNA molecule and transporting it to the location, then why don't we just modify both of these DNA. Why rely on HDR?

[@SabrinaBerberi]

Wow….. questo video è veramente impressionante

[@vaishnavig6559]

this video is damn amazing

[@TheGuy..]

This repair capability seems to point to purposeful engineering. Instead of the cell just dying, how would it "know" that it needed to be repaired? How does there just happen to be a spare template around to copy from? Where would the information come from to direct the repairs? Where would the just-right enzymes come from to assist in the repairs? Too many coincidentals for a working repair system to be in place at once. Good video.

[@lonelyelectron5283]

cells have their own grup/class of surveillance protein known as MRN they expressed by cells over time no matter if theres any break or not, these group of protein Patrol in the nucleus, scann every piece of ur DNA strand to catch DNA break, once it catch, it trigger many chain reaction in ur cells, thats how cells know if theres any break in its own DNA or not

[@TheGuy..]

@@lonelyelectron5283 I understand that it works and also realize that there are successive chain interactions to make it work...sort of like ladder logic...something that we use in electronic programmable control systems. My curiosity is how people can think that this working system can come about to be operational through successive natural processes. Or, how did the cell fabricate the properly matched MRN protein/enzymes. Also, a transport complex and molecular machines would be necessary to build and transport the replacement segment. One would think that if a cell just happened to naturally come to be, then if it did not yet have a functional repair system, that it would just die out. There's just too many steps and complex enzymes to just arrange themselves without an engineer involved in the first place. If I misunderstood you I'm sorry. I can't tell for sure where your sentences begin and end.

[@İbrahimBal-g2p]

Hello, i want to ask you something, could CRISPR Technology be a solution for clinefertel syndrome (47 xxy)?

[@Shilpa-v7m]

BRILLIANT

[@lonelyelectron5283]

my Question is how cells choose given DNA template over its sister DNA template in HR

[@TheGuy..]

I wouldn't think that they "decide". It's all programmed in the regulatory networks.

[@gregorysmith1134]

It is first come first serve. The donor repair template reacts to the break faster than the natural process does. Think of it as a can of prepared biscuits versus those you have to make yourself from scratch.