How individual compound is identified in GCMS

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shirwansany

4 жыл бұрын

This video explains how individual compound is identified in GCMS

Пікірлер: 40

@iqbali8944 4 ай бұрын

Can we identify compound on the basis of retention time ?

@shirwansany8706 4 ай бұрын

With GC/MS, you can identify a compound with (2) Mass spectra of the compound and (2) retention time.

@ismartiismarti-rj5gn 3 ай бұрын

Thanks Dr Shirwan

@rajendraregmi5407 2 ай бұрын

When you select some peak so many compounds were shown, how to conform the given peak is of respective compound

@shirwansany 2 ай бұрын

There are a few ways to confirm. 1. Confirm with the standard of the compounds. Run a standard of the compound, record and compare the mass spectra. 2. Based on the mass spectra of the compound, confirm the mass of ion fragments

@rajendraregmi5407 2 ай бұрын

@@shirwansany I mean when you click some peak many mass spectra is shown? I need to identify Z-7-dodecyl acetate ? how can i do that? Anyways thanks for your video.

@shivaaleyasin6256 2 жыл бұрын

thank you in advance for your explanation. I don't have experience working GC-MS .after watching in the video I have a question . output of GC-Ms is chromatogram that shows abundance versus M/Z that molecular weight is calculated . how to calculate concentration of compound?

@shirwansany8706 2 жыл бұрын

Hello Shiva, thank you for the question. I have uploaded a video on determining the concentration of compounds via the GCMS here: kzbin.info/www/bejne/injUoIOCoLShd5Y Hope it answers your question. Thank you for watching this video.

@akiamini4006 Жыл бұрын

Very nice

@scientiainquisitor5391 Жыл бұрын

Hi there are multiple compounds detected per peak So which one will be the compound corresponding to the peak. Are all the compounds are said to be present in the single peak (whichever is detected).

@rahanjdas5572 3 ай бұрын

hello did you get the ans for this question???? i am also confused

@rajendraregmi5407 2 ай бұрын

I have the same question as well.

@scientiainquisitor5391 2 ай бұрын

@@rahanjdas5572 nope

@rahanjdas5572 2 ай бұрын

@@scientiainquisitor5391 thanks for letting me know

@YoHaAnN666 2 күн бұрын

You'll never be able to be 100% sure. Here, the mass fragmentation pattern (and retention time I guess) is (are) compared to the library to find the best matches. You can see a value to the right of the proposed molecules (in the column called "qual" for "quality") which is a percentage score. The closer it is to 100, the better the match between the data acquired and the library. Also, here are displayed only the 3 best results from the library but there are more for sure. In that case, the quality score is high enough to be fairly certain of the identity of the molecule, but sometimes, when the sample is more complex (and when you have co-elution of several compounds), the library struggles to find good matches and it can be useful to consider more possible matches.

@shawonislam2724 11 ай бұрын

Very helpful

@MahnoorUsman-je7te 2 ай бұрын

I have a question that how can we get this gc ms library???? and which software are you using??? Thank you in advance.

@TheMeemkim 3 жыл бұрын

Thanks!

@TheMeemkim 2 жыл бұрын

Walaikum Salam :-)

@syarifahyantiastryna104 11 ай бұрын

Belajar lagi❤❤

@nazishakhtar2 Жыл бұрын

Wslam sir! I have to compare compounds in plants of 4 different groups, is GCMS can interpret the different concentrations of the same compound in different groups of plant

@shirwansany8706 Жыл бұрын

Salam, GCMS can identify the volatile compounds in the plant. Here is the strategy: 1. Analyze the plant extracts via GCMS. Determine the detected compounds, qualitatively. 2. Based on the detected compounds, purchase the compound standards. 3. Analyze the standards via GCMS and set up a calibration curve for each standard. 4. Analyze the plant extracts. Then quantify each compound using the calibration curve.

@giuseppezedde4553 2 жыл бұрын

Thanks so much for your explanation. I have a question for you. Is the mass spectrum sufficient to identify a compound or is it also necessary to have the IR, NMR and UV spectra? Thank you

@shirwansany8706 2 жыл бұрын

Hi, It depends on the aim of the study as follows: 1. Shall you need to identify a new compound, mass spectrum, IR and NMR are needed. 2. Shall you need to confirm the presence of an already known compound, get the mass spectrum and verify it with the compound standard are recommended. Thank you for watching the video.

@giuseppezedde4553 2 жыл бұрын

@@shirwansany8706 I thank you so much!

@samsoonmaharifa6687 3 жыл бұрын

Walaikum mussalam sir The library is showing three hits What we have to consider, only the first hit alone or all the hits Because it shows different compound name If it is same we can take that without doubting, hit is showing three different compounds for a particular peak Please help me in this

@shirwansany8706 3 жыл бұрын

Hi Samsoon. In my case, if the extract is crude, I don't consider the hits as the correct result. Then, I submit the sample to another detection method to identify the compound. If the extract is the pure one, we could possibly accept the first hit; however, please bear in mind, the FTIR only identifies the functional group, not the actual chemical compound.