How To Clone Mushrooms

[@thombaz]

Banana for scale, good old times.

[@101Skywatcher]

I understood that reverence

[@mintyfresh2620]

Remote control or coke can also works lol

[@nopeno2350]

Lol

[@andrewgoodbody2121]

One of us! One of us!

[@michaelhurlburt4502]

I always used a AA battery

[@microska2656]

This guy looks straight into your soul

[@Ankh_Hippy]

Swampert D. I was literally about to post this 😭😪

[@johnpilapil9250]

@@Ankh_Hippy dude same here!

[@imamoronand9199]

Or slightly above it

[@skippydeenice]

it's the mushrooms

[@Ankh_Hippy]

Chip Osgood he’s seeing the souls of millions and it’s working through the lens ...mushrooms are dope

[@LauraMartinez-rf3dm]

I'm so happy I found your videos. I'm a science teacher trying to get my students interested in outdoors and gardening. I think they would be fascinated with growing mushrooms. Also I want to learn myself :) I'm going to check out your website. Any suggestions for first time growing? Thank u!

[@FreshCapMushrooms]

Awesome! Glad you like them :) My suggestion would be to start with a "kit" to get an idea for the process- then it's down the rabbit hole- happy growing...

[@bananabreadyum9663]

I wish you were my teacher! Greatings from germany

[@Chris-dy5fy]

Invest in doing things right. If u invest in the right substrates, sterilization procedures, flowhoods or glove box, obtaining sterile cultures... It becomes very easy. It's difficult if u cut corners, and mistakes will cost u more down the road then investing in proper procedure.

[@TheMldytwkr85]

I wish I would've had you as a science teacher. My current Biology Professor down plays anything on mushrooms, in fact he gets annoyed whenever I bring up fungi or mycology. I kinda feel most of the science community has a superiority complex when it comes down to the mycology department?🤷‍♂️

[@caseywillie443]

About time man you needed to have done this video long ago. I have been wondering how to do this, Thanks!

[@FreshCapMushrooms]

Your right, I've been slacking!

[@tomfoolery8100]

​@@FreshCapMushrooms how much higher is contamination rate if i do it in a plastic box that i cut 2 holes in for handling.

[@donutkillmehh]

Flow discipline that you were mentioning is something that never occurred to me. That's really smart keeping where you're going with your transfer/clone etc as close as you can to the hood.

[@Cheddarbob32]

Couple questions: Is this tryptic soy agar? Incubate at room temp? How long are the plates good for when stored in fridge or freezer?

[@filax4500]

The amount of eye contact in this video is proportionally equal to its awesomeness!

[@woody9283]

Thanks for showing what contamination looks like. No one ever shows what to watch out for.

[@kaorumugen991]

Yeah, but it kind of makes sense that they don't. For every contaminant someone shows you, there's a thousand others they can't. A much better way is to simply learn what healthy mushroom mycelium looks like, then assume everything else is a contaminant.

[@robcroyle]

Best mushroom videos on KZbin! Every mushroom lover and grower should watch your vids! Video suggestion; how to make a spore print/spore seringe and how to properly store them.

[@abdullahzayadin4671]

Can you explain what is the next step in the process? And did you find any useful data answering your question?

[@robcroyle]

@@abdullahzayadin4671 I'm not sure I understand what your asking? As far as making spore prints my research has taught me, you take the mushroom cut off the stalk/stem with sterile scissors or a scalpel, making sure to cut it flush so the cap can rest on the paper, then put a drop of water on the top of the cap and place a glass over the whole thing overnight. To make spore syringes you take the spore prints and put them in the desired amount of water and allow the spores to leave the paper and become suspended in the water. Finally sucking the water up into the sterile syringe. As for storing them I'm still not sure, all I know is keep them out of light and high heat

[@dg5450]

For active mushrooms check out arvin fortyseven

[@daraa151]

jackson.wilmes Absolutely, this is the best and most useful videos on mushrooms I have ever came across! Thank you and keep the good work!

[@tarabonee8855]

Great video...would love to see a video explaining cleaning up a culture also. Oh! I just got the tshirt I ordered in, and duuuudde...I love it! Super quality

[@tarabonee8855]

I just saw the transfer video, lol. I must have missed it before.

[@overratedprogrammer]

You should make a video on maintaining/storing cultures, spawns, and mushrooms

[@chrysovalantislamprianidis8893]

Hi and thanks for the videos. They are really instructional and easy to understand.Maybe you answered this one already. Where can we store, what temperature and for how long both the empty agar dishes and the ones containing mycelium.

[@Budnbuf]

Excellent. Anthony you are so good at spreading the word. Thank you and good job.

[@klean8198]

What type of conditions do you leave the agar plates to grow? Like temp, lighting, etc.

[@matthewkbikes6845]

Temp is about 21 - 27 degrees C, not sure what it is in Fahrenheit

[@Maolo92]

1. How do you store the agar plates? 2. How long can you store an healthy culture?

[@princetonsv8126]

After you did cloned, where did you keep the plate? in room temperature or in the fridge?

[@sadhu7191]

You eat it. Did you even watch vid?

[@danielcrawlson1174]

@@sadhu7191 I watched the video twice at no point does he mention the conditions after the cloning process. where do you keep them while its growing? and what about storage conditions after? And whats the air stream thing called I couldn't understand him with the thing turned on

[@salmansadeq1167]

@@danielcrawlson1174 The thing is a laminar flow hood. You should look up the ideal incubation temperature for the specific species you're cloning, but room temp is usually fine. Not sure about storage. I haven't actually started cultivating yet.

[@rustyvessel5086]

@sadhu I ate it. Nothing man

[@Liliisntiiriis]

Is it better than doing an spore syringe? Could you make a video about pros and cons of each? BTW... I started this rabbit hole because of your videos! Thank you!

[@rushinbushin]

The precision makes me so happy

[@DDawg1144]

You’ve got a great channel, already made grains thanks to your vid, soon will graduate to agar.

[@emmanuelsebastiao3176]

Thanks for Sharing Tony ... You are a genius. And i Love how you've done your operations so carefully. you are a Champion

[@chenjiachen5547]

Where do you store the Petri dishes for further culture? An incubator ? After the mycelium fully takes over, how to store them for long-term use?

[@philq01]

Can you reuse the petri dish? Or must you just throw it away?clean it with alcohol and or expose it to uv light?

[@chanteuhtlicuauhtlatoa6115]

Thak you so much for making these videos!! I have some queations; after you put the mushroom pieces on the agar in Petri dishes, how much days are one going to expect them to be ready, and how is it preferable to store the petri deshis, like in a dark fresh place, with no iight coming in? Then, after we have the spores blooming, can we make them into a liquid cultive?

[@haangloose]

Great video! Where Do i have to store the Agar plates with the tissue inside?

[@Filekv5]

I love the content you put out. Keep doi g the great job!

[@FreshCapMushrooms]

Thanks so much!!

[@mycoalliance1909]

Awesome! I shared this with students from my recent class. Very helpful info. Keep it up =)

[@FreshCapMushrooms]

Thanks!! I will definitely keep it up 😀

[@davidmorrisii69]

Nice video brother, i really appreciate it!!

[@johanneshalberstadt3663]

Couldn't you also directly take pieces of mycelium to put on the agar?

[@jcjensenllc]

You didnt explain the bag holding Petri dish. Does your culture need to be at a specific temp or humidity?

[@fishmut]

Simply Human .id say doing it at room temperature when the agar has set in the Petri dish then add the culture is my guess from watching the video , Research your mushroom type your would be doing. Some like cool temps and others warm to hot ,you will have to work it out do some homework.

[@frederickscutts9780]

Wheres the best place to store the dishes in order to encourage the mycelium to grow?

[@aleksgregorcic3868]

Dark warm place (around 23-27℃)

[@daraa151]

You are my hero now! Can you please show us how to prepare liquid cultures?

[@aleksgregorcic3868]

You need to get mushroom spores and mix them with sterilized water

[@naesism]

Hello, thank you for the informative video. I tried the king oyster and it worked, but not for portobellos and enokis. Any suggestions for why that might be the case?

[@calisav223]

Wondering what temperature the agar plates are to be kept at for growth? Interesting stuff

[@aleksgregorcic3868]

For growing i would say 25-30℃, for storing i would say 2-5℃

[@Axelrun1994]

Can you clone by just putting a scrape of mushroom right into a jar full of sterile substrate to make a cake/spawn or does it have to be mycelium? I know you want agar so that you reduce the amount of possible waste from a contamination but hypothetically speaking...?

[@FreshCapMushrooms]

That is definitely possible in theory- but might not work so well in practice. It can be done though, people do it to make "cardboard spawn" by simply taking pieces of the mushroom stem but and placing in wet cardboard. The above method is much more likely to succeed though.

[@hashtastic3537]

I know it's possible, I've seen pics from other guys who've used a chunk of mushroom to clone. I thing your best bet would be to take a chunk out of the inner flesh of a mushroom while in front of a hood or inside a glove box then place on your sterile agar. Of course your looking at an uphill battle, the guy I'm thinking of likes to show off his skill. I've seen him grow on a layer of $20 bills in a foil tray.

[@Axelrun1994]

@@FreshCapMushrooms thank you very much for your reply, I haven't gotten a notification. I appreciate the advice

[@latinkaellis152]

@@FreshCapMushrooms Thank you so much for all the videos you have made to help,they are very informative,brilliant! :)

[@allandehn]

@@hashtastic3537 Do you happen to have a link to this gentleman's channel?

[@deniskarateev7171]

Wonderful video! By any chance, is it possible to clone this ways wild mushrooms such as Boletus Edulis or Lactarius Deliciosus?

[@Mycostinzonig]

Mycospinz

[@Mycostinzonig]

On IG

[@OmniClimHazard]

Love these vids! Question. How long does an agar culture last/ whats the best way to store it?

[@aleksgregorcic3868]

Fridge. I am not sure how long can they last tho

[@user-jn7nf6xu8l]

after complete colonization of the mycelium on the entire surface of the agar, where and at what temperature and how long can the mycelium be stored?

[@shenyanbin]

Is there a difference between a mother culture created from cloning a mushroom and a mother culture made with spores? Would there be difference in term of yield and quality of the final product? Thanks

[@rustyvessel5086]

Damn good question. I've been asking that on other channels as well. I'm betting I'll do research and get back to u on it

[@ShotgunLlama]

What do you do with the agar dish once they have a culture? Do you just put the agar directly into a sterilized substrate and let it grow from there?

[@hgrunenwald]

I am wondering the same thing-where do you go with the culture once you get to that point? I noticed your question is from a year ago; curious as to whether you found this out. I will do a little more digging online...

[@ShotgunLlama]

@@hgrunenwald never really did. Sorry. Got a couple oysters to grow once in some cardboard but nothing big

[@Redneck_Renaissance]

I'm intrigued in making and using agar plates to clone per your previous how to video........when a plate produces pure strain can you sterilize and reuse after or should you always throw them out? Or do you just throw out the contaminated plates because opening them would contaminate as you mentioned in this video?

[@FreshCapMushrooms]

Hey there! I use disposable plates, they come presterilized, just add the sterilized agar. I wouldn't bother to reuse them, just not worth the hassle, unless you have glass plates, but again, probably not worth it. And ya, when a plate is super contaminated I would rather just not even open it.

[@Redneck_Renaissance]

thanks for the advice.....I'm going to buy my supplies from you.

[@philso7872]

Do you just tie up the contaminated plates and other contaminated materials in a plastic bag and put it in the household trash or do you take some other precautions?

[@ambientaldrone]

Very helpful. Just a quick one, the ziplock is really necessary considering you already sealed it with the parafilm? Doesn`t it stop the oxygen to get inside? Cheers from brazil.

[@aleksgregorcic3868]

It is better to put them in a bag just to make sure as little contaminations can get in

[@Aethelvlad]

how much agar powder/water do you use in a dish this size?

[@pauldolton9118]

How long will these last in the culture pots and where do you store them?

[@aleksgregorcic3868]

They are stored in a fridge

[@lizziebee315]

Hi. Please advise as to the food substrate that you added to the agar.

[@aleksgregorcic3868]

He posted recipe. Or if you are looking for cheap homemade recipe here it is: 2g salt 1-1.5g yeast extract (or vegemite or anything like that) 6.5g gelatin powder (basic one, without sugar or flavors)

[@paulprior5846]

Great work Tony! Can anyone tell me where I can buy the rack to place the plates on? I’ve looked everywhere!

[@Vodafone16v]

I was going to ask the same! Need one of those Stainless Steel racks!

[@salmansadeq1167]

@@Vodafone16v It's just from Home Depot or something.

[@ljamest2112]

Thanks Tony!

[@dariuszwesierski1928]

Can i clone mycelium from fruit body straight to sterilized grain? Like You on agar plate, take 3 pcs and throw them to jar

[@ThatBritishHomestead]

if you grow them in deep multch and keep adding to in with they ever stop growing?

[@seraj.]

How can you store it and how long does it last?, great video btw

[@randymartosoewito7892]

Can you do this with dried mushroom also?

[@BigBodyBiggolo]

Try it, people been saying you cant do this and cant do that but theb 20 years later its a genuine tek. Dont know about dried mushroom myself but why not right?

[@bony3679]

Soak them?

[@SAMI-gc1hx]

How do you prepare the agar mixture? Apart from agar what else do you have to put into it?

[@pattybb2823]

Thanks for the video. Are you scraping from the flesh or gills? Does it matter if it’s either?

[@jadenbroadway173]

What’s a good temp for incubation?

[@mihailmihaylov4974]

Could tell me what would be the best way to preserve mycelium or spores long-term? Keeping grain spawn / liquid spores / agar dishes / print? My hypothesis would be a spore print taken in a sterile environment and kept in dry sealed jar.

[@and7barton]

Information is very good - Music is not good. I've been growing mushroom for a year or so. I'm going to try the cloning process.

[@creatodayin4924]

Great helpful video but sir how I store the plates and how long I use a culture for Micilium growth? Lots of love from Asia

[@jakebeaker4243]

Does that work for magic mushrooms aswell?

[@jonathanfreed5745]

Where did you get the little rack for flowhood

[@SunnieDIY]

How long do they last and where to store them once it has developed on the new dish

[@chucky2959]

That oyster looks so yummy ~

[@Al_i8]

thanks Tony

[@mirzarashidbaig]

Love your videos. Just curious to know that how many days does it take to fully colonise.

[@FreshCapMushrooms]

Thanks! Depends on the species, anywhere from 1 -2 weeks is typical.

[@xxpowwowbluexx]

If there’s a tiny bit of contamination, would the mycelium potentially grow into and break down the contamination, given enough time?

[@sacacorchosbarravel4909]

Depends on the strain of the mycelium. In my experience, some mycelium types do indeed win the fight, some do not.

[@AnonAnon-oj1rt]

I’m confused, what do you have as a result of doing this? Does it turn into mushrooms? Or are spores the result? And if spores are the result, can you put the spores in a syringe and use them to grow mushrooms? I’ve heard the stuff inside of a spore syringe be called spore solution or liquid culture, if spores are what you do have as a result of doing the steps in this video, and if you do put the spores inside of a syringe, do you have to mix anything with them to create spore solution or liquid culture before putting them into a syringe?

[@aleksgregorcic3868]

Once mycelium grows you can take few pieces out and put them in grain. Liquid culture is mix of spores and destiled water and not mycelium, but you can still play around and try if it will work

[@markmiller5004]

Liquid culture is mycelium suspended in a sterile nutritious medium. And a spore syringe is spores suspended in sterile water.

[@bryanadz]

Hi, How long time you can conserve the Liquid culture and the discs and where ? Thanks

[@chrismartin5010]

How many times is it possible to ”reclone” ? Meaning you manage to get the fruiting bodies an repeat the operation indefinitely?

[@marlonmoore5000]

Cool it in the dish? Is there water in it? You never stated.

[@nuflix320]

Hi. I've never tried growing mushrooms before. I don't have access to most of the scientific stuff in my country such as petri dishes. Is it possible to clone a store bought mushroom just by using stuff like jars, pressure cooker and homemade still air box. I just want to start growing on a small scale to enjoy the process at home. I also saw some guy grow store bought mushroom on coffee grains in a Jar, idk what was the process called exactly but something like that would be great. I think it would also be a great idea to make a video about it for kids and people with same problem I have.

[@GameTesterBootCamp]

I realize this is likely the "best" way to do something like this but is it possible to just mince up a mushroom and put it in some mulch/wood chips and get some more mushrooms that way? Is it even possible to clone that way?

[@algonzalez6853]

waiting for that answer

[@AnonAnon-oj1rt]

Could you use just see through tape instead of parafilm?

[@aleksgregorcic3868]

You can use that paper ducktape that is used for protection when painting rooms

[@guardianofthesacredflame1649]

Is it best to take the tissue from the cap or the stem?

[@asthmaenthusiast]

Wouldn't the donor mushroom remain edible if cleaned with ethanol? Grain neutral spirits, for instance.

[@datnguyen-ul9vm]

I love your videos but it would be nice if you making a video processing of the mushrooms grown

[@williamkreth]

Thanks for sharing your knowledge dude appreciate it

[@M21zen]

Excellent. Did you cut the strips of parafilm or buy them that way? I wish I would have thought of that a few years ago. lol

[@berger77]

I use to cut plastic wrap rolls to two inch lengths. This was before amazon and didn't work well as I had a lot of problems with contamination. I see amazon does have some parafilm but a science supply might be cheaper.

[@FreshCapMushrooms]

Hey there! Yes, it comes in a big roll, 1 inch wide, I cut the strips with scissors and have them ready. A big roll lasts a long time

[@revolution5547]

Would it be possible to use a still air box instead of laminar flow hood

[@shaimabukhamseen6451]

Hi, thank you so much for sharing, can I do it with button mushroom ?

[@dzdzful]

Thank you so much, Now I have to find out how to grow mushroom using this culture on petri dish...

[@fredrikschubert2692]

Such great videos! Could you put a tissue sample straight into liquid media and expect mycelium growth or is that more likely to result in contaminations?

[@dylanjones7798]

Old comment, but if you're still wondering... It's entirely possible, yes.

[@ramonlarson6806]

Also do you have to keep the culture dishes in an incubator or anything?

[@dillicous136]

can you reuse agar plates if there's contamination? like clean them out and reuse them after re-sterilizing them with alcohol or peroxide or something? same question for fruiting block bags....

[@RachaelBally]

You can reuse plates after sterilization, but you have to create new agar agar solution for use with the plate

[@noventainueve6633]

how long can you store it from those dish sir?

[@Mycostinzonig]

On IG

[@Mycostinzonig]

✅✅

[@iweirjkdkfmk]

Doesn't putting three things in your agar plate multiple your contam chances?

[@dialecticalmonist3405]

When you say, "contamination," does this always mean "mold," or is there another form of "contamination"?

[@FreshCapMushrooms]

Typically trichoderma mold which is very prevelant, but could also be bacterial contamination.

[@AndAbel-AOD]

Bacteria and mold.

[@axsanchez1193]

If I clone a mushroom and have a good mycelium, fast and powerful. Then I pick the bigger or the faster specimen and repeat the process, the strain will be even more powerful? Can I call it Cloning Second Generation? Even a Third Generation is is possible or the beneficts of cloning are just for one time? Good vibes from México!

[@FreshCapMushrooms]

You could definitely start to select traits from genetic variations, and clone fruits to continue developing a strain, but that would be a little more complex than what we are doing here. Basically, this is just making a genetic copy of the mycelium that created the fruit body. Super valuable for creating wild clones, probably less important when cloning fruits from commercial strains, like this one.

[@lucadavidson3936]

I'm just getting started in mushroom culture, but as I understand this particular technique and A-level biology, what you're thinking is not the case. The cloned strain would not be stronger or more powerful, because it's just a meiotic clone, so there would be no genetic variation created by the process. You're replicating exactly the traits from the parent to the child. It's a lot like fruit tree culture I think.

[@SouthtownHound]

Look into Stamets P-value. It may help you keep track of your generations.

[@FreshCapMushrooms]

Yes, this is a good way to do it! Still can be hard to keep track though lol

[@bretdaley6869]

Do you build your own laminar flow hoods?

[@Cbarnes256]

He does he also has some instructional videos on his channel and info on their website.

[@nahceil]

How about smaller mushrooms like pan cyan? Can you still get a tissue on it for agar and get mycelium spores?

[@mrrrl795]

with the culture, could you then just transfer it to a grain spawn and then to a substrate? or could you go straight to a substrate?

[@jadechanges1379]

you typically cut some pieces out of the myceliated agar and go to grain spawn.

[@acykat2069]

There is a tiny reishi mushroom growing on a hackberry stump in my yard, but the surface of the stump is completely white in a patch--it looks like a block getting ready to fruit. Being January, it's growing very slowly.. is it enough mushroom to cut off for a tissue culture, or do I need to wait? There's maybe a half inch of red showing on the fruit body.

[@raymondhunter2401]

Do you need a flow hood for this?

[@mikehunt4797]

Wouldnt more pieces per dish also increase the chances for contamination vs 1 piece?

[@quickchop1098]

Are you able to do the same Process but instead of an agar dish, can it be straight into a Liquid?

[@ramonlarson6806]

When you do transfers do you just try to cut the good pieces out?

[@trevorvanzuydam8115]

Does the alcohol (and fumes ) kill spores or the shroom body?

[@tatanleanduck]

Is it true that cloning from fruits that aren't fully mature (still growing) will provide you more aggressive mycelium on agar? I read that somewhere, but it doesn't seem to make much sense to me. The aggressiveness of the mycelium should be based on the genetics of the fruit, not its life stage, right?

[@CMAenergy]

I'm not familiar with those dishes,What is in them that allows the my-cilium to grow, Can we make this easily

[@jameswilson5533]

Agar is in them, the dishes are called petri dishes. Agar plates can be bought pre-made or you can make your own using agar agar and something like potato dextrose or light malt extract mixed with water. :)

[@CMAenergy]

@@jameswilson5533 Thanks, I just hope your method of propagating mushrooms actually works, as I've never seen anyone remove the bases of mushrooms to do this, as no one has ever expressed it or shown me that, I'm going to try it, and mushroom dealers here I contacted requesting some spores and they never got back to me, Thanks and I really hope this works,

[@lorenzovega8716]

Dude such great vids. This guy is great!

[@dorfriedman9115]

Is it possible to skip the Petri dish phase and just put a piece of sterile steam into the ready grain?

[@liamfbules2999]

Could you use high proof ethanol instead of isopropyl alcohol to avoid making the mushroom inedible? Or does isopropyl just work a lot better than ethanol.

[@Coolestnogar-ot6mt]

Is this normal agar or agar with added nutriënts?