How to Determine your Blood Type Using EldonCard

[UPDATE: I recently used spring-loaded lancets for this lab. They worked great. Here's a video showing my experience with them: • How to Use a Spring-Lo... This is great to show kids to take away the fear of using this unknown object. :) ]
I made this video to show my students my experience with the EldonCard. After I did this experiment with around 75 students, I have some tips that I don't show in this video.
1) If a person is helping you (which I highly recommend) have that person wear gloves.
2) When disinfecting with the alcohol swab, use an outward circular motion (instead of sweeping microorganisms back onto your finger)
3) Use a pipette to get the one drop of water. (Don't use the bottle that Carolina provides)
4) Be properly hydrated before pricking your finger (this allows your blood to flow faster after being pricked)
5) Have everything ready and to do the steps in the correct order instead of by doing by memory (this caused the students' wounds to clot before getting out the blood)
6) The person that is pricked should squeeze out his blood on the EldonStick. The person helping should hold the EldonStick for them while they do this
7) Prick the finger towards the side of the finger, rather than the center. I think this might cause less pain since less nerves are located there.
8) To help get blood out quickly, after being pricked, make sure you are standing up. Place your arm with elbow upwards and hand pointed at the ground (like an upside down "L"). Then with your other hand, make a very tight fist on the pricked finger and squeeze tightly downwards, like you are trying to milk a cow.
9) TILT THE CARD IN THE CORRECT ORDER. The one thing that really messed up some of my students' results is that they didn't tilt upside down first and then right side up. If tilted incorrectly, the water in one field can spill into another field, causing the antibodies from both fields to mix and the results to be inaccurate.
10) Protect your results using clear packing tape if your card doesn't come with "fixation foil"
11) If someone faints at the sight of blood (I had a student faint while watching the instructional video) be sure to allow that person to lay on the ground until they recover. Picking up their legs also helps restore blood flow back to the brain .
12) Using lancets from a glucose meter doesn't work because the prick is way too small to get a decent amount of blood out. Use the lancet that comes with your kit.
If you want a single EldonCard, I found this link on Amazon for a card that costs $11.95
www.amazon.com/Blood-Type-Kit-...
If you're interested, this is the EldonCard kit that I bought from Carolina if you need a class kit of 25.
www.carolina.com/product/abo+r...
I don't recommend this kit for a few reasons.
1) The bottles they give (in place of the micropipette) are useless. Not to mention, they give 4 of these bottles for a class of 25 students which makes no sense to me.
2) They provided a DVD for both of the kits I had ordered, which just seemed wasteful. I thought this should be sold separately, since I now have 2 identical DVDs
3) The DVD shows items provided with this kit that aren't provided by Carolina, like a protective cover for your results, a cotton ball, and most importantly, the pipettes!
(I'd rather order a kit that comes with the protective cover, a.k.a. fixation foil, for each card; however, I've provided packing tape for my students to tape up their cards which works just as fine I believe.)

[@bruteshade]

Hey guys, a tip for more clear results; When "tilting" the testing paper, just slightly tilt and do not allow the mixture to run off of the test paper. After only slightly tilting in all directions, allow the mixture to dry. This will give a more clear result for reading and I suspect your positive RH factor result would be more evident. Good video, I used it to show my class! Thanks!! :)

[@AquaVlogs9001]

I watched this video before. It was so hilarious. Enjoy a new subscriber.

[@Mojoremixxx]

that was beautifully demonstrated :)

[@Xolette]

Real good point. Thank you!

[@Xolette]

Yes! The side of the fingertip is better than the middle. :) Thanks for sharing!

[@Xolette]

Thanks for the advice! My students are about to do this lab again, so any tip on how to help them get the blood out helps. :)

[@Xolette]

Yes, I forgot. But I had no open cuts and washed my hands very well afterwards.

[@XoletteScience]

Thank you!

[@abandonedaccount1643]

For anyone confused... Reaction to Anti A: *Blood Type A* Reaction to Anti B: *Blood Type B* Reaction to Anti A and B: *Blood Type AB* Reaction to Anti D: *Positive Blood Type* No Reaction to Anti D: *Negative Blood Type* Reaction to Control: You made a mistake For an example, if you had a reaction to circle Anti B, and circle Anti D, you would be a B+.

[@No_name860]

Dead Channel what if you are 0

[@JessicaLister899]

@@No_name860 if you are O, you won't have a reaction in A or B. And the Anti D will still tell you if you are positive or negative.

[@darthmichael12]

Very interesting indeed!

[@TorchySmurf]

These are so fun! Great for Holloween, 😳

[@blackonix13]

I'm so glad that I found this video because my blood type kit is due in the mail any day now, and I have no idea how to do it. haha My Biology professor said that we can't do this in class because it would get too messy and we cannot have people's blood all over the lab stations. Fortunately I'm doing it at home and sharing my results for fun.

[@Xolette]

Good for you! Be sure to read the description for more tips. I was also afraid to do this lab with students but it was by far one of the most engaging labs for my kids. I only did this lab too after so many requests. Best of luck to you!!

[@coyner080]

Xolette Have you ever had a professional give you your blood type? If so, was it is same as on the EldonCard?

[@Xolette]

Jake C Yup. The EldonCards work if that's what you're asking. I've also had students report the same thing. As long as you follow the directions, it'll give you an accurate reading.

[@coyner080]

Xolette well that is good news... thanks

[@blackonix13]

My parents said that they knew my blood type when I was a baby but they forgot through the years. I finally got my EldonCard in the mail, and it said that I'm A Positive. Good thing that it wasn't done in a classroom setting, because my finger bled a lot more than expected.

[@Xolette]

When it's dry, compare the Control to the D circle-- if they look similar (no clotting) then you're definitely be A-. it's possible for you to be A- with an A- parent and A+ parent. Your A+ parent could also be a carrier for Rh- factor (as Rh+ is a dominant). On a side note, being Rh- means that if you do have a child, you have to be wary of Rh disease, in which if your baby is Rh+, your body seeing the Rh+ fetal blood as foreign, will attack the baby.

[@sadella]

Xolette there is no contact between the baby's blood and the mother's in a normal pregnancy. In some pregnancies, some conditions like extra uterine pregnancy can lead to the baby's blood entering the mother's system. Then the rejection would happen. But in normal pregnancies there is no issue having a baby with a different rh.

[@NortelGeek]

Just took this test from Amazon. The instructions packet was not as flashy or nice but they give you an automatic lancet.

[@BINHNGUYEN-ox6hc]

THANK YOU

[@followmei4789]

You needed more blood, but I'm guessing he's A, maybe A positive. I'm glad that you did this because I was thinking about buying one to test my blood.

[@Xolette]

Yes, this is all you need. I saw on amazon that an EldonCard costs about $11 - $15. I can't believe it costs $150 in a lab. Make sure to read in the description to this link tips on how to help you get blood out once your finger is pricked. My first class had a hard time and then I figured out what they were doing wrong. :)

[@iminco9844]

Its $300 now.

[@XoletteLife]

@@iminco9844Wow, healthcare really rips you off!

[@Xolette]

Btw, what I meant by "identify as foreign" is that when your body recognizes something doesn't belong, be it virus, bacteria, and in this case, someone else's blood, your body will go into attack mode in which antibodies will attach to the foreign blood cells which results in massive clumping of the blood cells, which in turn, can lead to death due do blood not getting to where it needs to get to.

[@ljl787]

Good Show......

[@XoletteScience]

Thanks :)

[@ExplosiveFetus]

When you disinfect with alcohol swabs you should rub in circles around the area, not wipe straight across. Wiping straight across can reintroduce organisms back into the area.

[@Xolette]

Being "negative" means you also don't have another marker on your blood cells, which is called the "Rh antigen" or "Rh factor". This means that your blood can be accepted by both positive and negative people, whereas, someone with O+ blood can only donate to only positive people (like A+, B+, AB+, and of course O+). Definitely donate your blood as it most likely would save a life!

[@itsayskeds13]

I have the same blood type, A+!

[@Xolette]

Mr. Stevens just chuckled at your comment :)

[@Xolette]

Yay! :)

[@lindseymew]

I just got this blood typing kit today because I've been very curious. One of my parents is A- and the other is A+. I did my test, and it very clearly showed up as A-. The A circle was very obviously clotting, and the others just looked like red liquid. But after two or three minutes, I moved the card while the circles were still wet, and the D circle sample separating. Like, the liquid started running away from itself. What does that mean? It certainly wasn't clots.

[@Maevelikeschampagne]

Lindsey Marie wow, the exact same thing just happened to me. I think the D antigen takes longer to clot, but I am uncertain as to negative or positve because I am unsure if the blood for antigen-D would have done that to a degree anyway?

[@athletejmv]

My control group clumped on the test... so I'm wondering what that means?

[@XoletteLife]

athletejmv It means you have to redo the test...I had it happen once with one of the kids, but I think her blood type still came out correct.

[@Xolette]

It is not impossible for you to be O- with you having 2 O+ parents; however, it'd be impossible if your parents were both O- and you came out O+. If you understood genotypes, it'd make more sense. For example, you are Rh-. That means you have 2 Rh- alleles ( - - ). One of those alleles (form of a trait) came from mom, the other from dad. In order to be Rh+, you can either be + + or + - . The Rh + alleles is dominant over the Rh - allele; in essence it masks over the Rh - trait.,

[@j.j.springer1099]

Needed to this live so 1000 people could scream with him

[@Xolette]

Universal Blood Donor who can donate to all blood types, yet can only receive blood from one blood type, O-, which btw, consists of 6% of the American population, whereas O+ is the most common at 37%. (A+ is 2nd most common at 34%) This blood type is used in emergencies since the person needn't be blood typed beforehand. Why O- blood doesn't agglutinate (clump) with other bloodtypes is because O blood doesn't have any surface markers (antigens) that identify it as foreign in other people.

[@firebad0]

I got my blood tested when i was 4, I ended up being AB+

[@Mr2tails]

Anyone heard of blood chimeras? Rhesus - and + blood of same type in one body...

[@dariusthehedgehog180califo9]

I think I have this.

[@Xolette]

Yes, he said that slowly poking his finger with the lancet wasn't a good idea. :)

[@tropicalwave780]

Is the fingertip the only place you can stick it or can you do it like on your earlobe or arm?

[@Xolette]

The fingertip is the best place... it's easier to direct the blood out of your finger, as opposed to your earlobe or your arm. Watch this video to see how my husband pushes blood downward out of his finger. How to Use a Spring-Loaded Lancet (for Blood Typing) Best of luck!

[@Xolette]

Btw, don't forget to hydrate (drink plenty of water) well before getting bloodtyped. My students who came dehydrated had a tough time getting blood on their eldonsticks.

[@omegablast]

mmm this is interesting... I've made some blood test with doctors and three times got 0 Rh - my family had their doubts since they have 0 Rh + I'm the only one that is negative... I live in México, and don't know if I can buy one of those test you showed in this video to make sure myself I'm Negative... but well it will be foolish not to be sure since I did three times the test with them hahaha Anyway it was funny to watch you.

[@abdualkhaligynb]

oh shitt you are so hoot doctor

[@hanoszprime5431]

I forgot to add drop of water to circles before I added my blood. Will it still work? Looks like I'm A- as it is.

[@XoletteLife]

Not sure. When my students made mistakes I could only give them a new test if I had extra. But if they were able to read their blood type, I'd say, "well, you might be ___!" In any case, if you can go to a blood drive, they also will tell you your blood type-- from my experience.

[@hanoszprime5431]

@@XoletteLife I tried it again, this time correctly, and it worked properly. So, it won't work if you forget to use water.

[@Xolette]

Doctors nowadays keep in mind the blood type of both mom and dad, so this doesn't happen. And if it does, there is medicine to suppress the mother's immune system from attacking the child. And yes, for your Dad to be Rh- and mom to be Rh+, you had a 50% chance of being either Rh- or Rh+.

[@ElectricAngel19]

thats exactly what happened with my pregnancies.. causing the loss of 6 children before the Docs would do anything, I am ORH- so what i am wondering is this... if my father was AB- and my mom A+ would that seriously = me?

[@ElectricAngel19]

@Noob Saibot lol!

[@Xolette]

That is the best blood type to be-- you are the universal recipient meaning you can receive anyone's blood. Unfortunately, the only blood type you can donate to is other AB+ people (which is practically unnecessary :) )

[@Aliofthesteeze]

My blood type

[@sselemaNrM]

Aaaaand the two people in the video clearly had chemistry...

[@visionette225]

where do u get these

[@cometeloortega9014]

Sorano Rose Black online Here: www.amazon.com/Eldoncard-Blood-Type-Test-Complete/dp/B00JFTSPMW/ref=sr_1_1?ie=UTF8&qid=1433620343&sr=8-1&keywords=blood+type+test+kit

[@cfystalsarchive]

im nervous laughing so much with my blood phobia

[@FUBAR956]

What if you're O-?

[@XoletteScience]

My boyfriend :)

[@ethernicagape3163]

A+, just like me !

[@shaz-johnthreesixteen3331]

What about O group type?

[@XoletteLife]

There'd be no agglutination in the Anti-A and Anti-B circle four blood type O.

[@renderz8435]

Is there a way to test without poking urself

[@mannys9130]

If you can get blood out of your body without lancing the skin, sure. Not sure how you'd do that though. Ebola?

[@8moltovivace8]

the ear lobe is a cartilage isn't it? so no blood will come 😦

[@Xolette]

The top is cartilage. You can get blood from the bottom of your ear but it was difficult for me.

[@8moltovivace8]

Xolette oh okay :D

[@hessy222]

living with biochem Girl should be interesting:

[@trishabiddle5175]

I found out I am either B- or AB-. Its very interesting

[@primarymedicalhealthcarepr4063]

RH... Blood... be cautious with that one.

[@Lerious]

Really like the dialog Xolette and her boyfriend have in this video! Mr. Stevens says, 'I really didn't sign up for this.' made me Lol.

[@18rickman]

lol why so formal

[@babykathful]

if you give blood or get test at GP like i did it was better to know your type proberly than ordering of amazon and im A RH positive welcome to the club sorry if people dont agree with me just my own opinion i wont get offended

[@DeeZeeTayy]

that's true but not everyone is eligible to give blood and idk what GP stands for but if you go to your doctor's and take blood they won't typically test for the blood type; some will if you ask though, but then you have to remember! lol

[@babykathful]

+DeeZeeTayy gp stands for general practice

[@babykathful]

+DeeZeeTayy I totally understand what you mean I would be scared to prick myself to find out what I am but I'm glad I gave blood and know what my blood type is so amazing to go through

[@MaCherieMcAli]

How about if you ever do that be more careful and use gloves.

[@ThePunisherNWO]

wippee thanks xolette for your guide im rh negative so 100% god :)

[@kevinr4275]

lol, don't tilt it that far!

[@Xolette]

There is medicine to prevent that though.

[@noahbrestovansky7716]

That's what she said I just des infected

[@asiantalim]

Lol, Should have been an abrupt poke on the finger like the way Jon did on your ear. Nice demo. =)

[@No_name860]

What if you have blood type O or AB?

[@XoletteLife]

Blood type O would show no clotting (agglutination) in the A and B field. Blood type AB would show agglutination in the A and B field.

[@marlonfoster6246]

she is so cute

[@dreamqq]

you didn't do it right you should put larger blood drop and you should wait two minutes before you start flipping the card.

[@Xolette]

This was my first time using the EldonCards and I learned a lot from that experience which helped me when teaching my students how to do it. Read my tips in the "About" section if you want to learn more. So far, I've done this lab two years, with about 100 students. There's no need to wait two minutes-- it doesn't say in the instructions. The blood typing works without the wait. What does matter is if you use too much water, which is what happened after I used the bottle they provided.

[@lamont1659]

Gloves😷😷😷🙈🙈🙈🙈🙈

[@1sgbrewer]

You should have thrown the lancet at his finger. He could have stood in front of your tgt board with fingers stretched out, ohh and you're blindfolded and laughing. Muahahahahaha!

[@carlitoxwey69]

😂😂😂 It says tilt, not turn over. 😭😭

[@cifordayz4491]

Awesome lesson in how to do blood work... WITHOUT GLOVES???? WTF!?

[@estherong3417]

You are +A

[@somerandomretard8283]

NEVER THE FUCKING INDEX!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! AND ONLY ON THE FUCKING SIDE!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

[@dagansgamingbox6604]

I literally couldn't get blood from anywhere else, aside from my ring finger on my opposite hand the second time around. First time I didn't get anywhere near enough blood. :I And I was using spring loaded, so you can imagine the fun of only being able to stick once.

[@babyboysmooth1353]

Now I'm not a squeamish person and I accidentally cut my fingers all the time but video is just whack

[@IkechukwuIheanacho143]

You guys are doing the test without hand gloves, u gonna transmit some diseases to each other.

[@mannys9130]

Take note of aseptic technique.

[@PartiZan1992]

Bad Luck Brian. Tests Blood Type. HIV-Positive.

[@fifamaniac04]

Touching other people's blood without gloves is TIGHT!

[@marlonfoster6246]

you are cute

[@Celestial_here]

Im a little disappointed. From the title, I was expecting you and Jon typing on a keyboard with bloody fingers. Whatever! :)

[@siyabongaradebe2833]

They aint even wearing gloves and thats bad for a tutorial lesson unless an individual is self testing

[@dragodemo9112]

Dis Like!!!!!! Too Scary!!! Bye.

[@estherong3417]

You Rh factory is weak

[@noahbrestovansky7716]

Some Chinese lady our any lady our person who's not a doctor smart idea

[@IndiSimpsonRomero]

Why would you use your ear ? you don't seem very smart , and you are NOT suppose to tip the card that far !!!!!

[@XoletteLife]

The instructions said to prick your fingertip or ear. The tilting works fine when a pipette drop of water is used. Read the description if you need more information as to how this worked out in my classroom.

[@IamAkoma]

This kit sucks... Bought 2 and followed all instructions and neither told me shit... Waste of time and money. Thumbs down.

[@XoletteLife]

No reaction (clotting)in all the fields, means you're O - .

[@snschink]

Who is he?