How to enhance fluorescence intensity in imageJ | Confocal images not showing fluorescence in imageJ

[@simonyacoub8262]

Thank you! This is exactly what I needed for lab!!!!! life saver

[@AliceritaE]

You're very much welcome.

[@sunnetinternationalbusines9910]

vielen Danke

[@AliceritaE]

Gerne!

[@b.9744]

Thank you so much!

[@AliceritaE]

You're welcome 😊

[@turkdogt6251]

Could you post a video on how to properly threshold to separate a cluster of cells to individual cells

[@AliceritaE]

Hello, I will work on that and hopefully upload the video by the weekend.

[@snehasishnag1324]

Can i use these adjustments in imageJ for publications?

[@AliceritaE]

Yes, but you have to add the adjustment to your materials and method section. E.g microscope images were further adjusted I'm imageJ to increase the intensity signal. Although, the image I showed in this tutorial is bright under the microscope, when I opened it in imageJ, it looks darker. The only edit I normally apply would be to use the RESET on the contract/brightness window. Then I get a brighter image and I don't have to add that yo material and methods