Measuring Intensity on ImageJ

University of Portland Summer of 2017 N171-82Q research
This is the protocol the Pitzer lab used to measure tyrosine hydroxylase intensity in ImageJ Software.

[@mcammer]

1. There is much faster way to do the ROI using the ROI Manager. Also, for mean intensity values, the ROI does not need to be the exact same size in all images, so maybe you could draw a new one for each image. 2. Doesn't the intensity measurement only need to be done in the green channel, not all three channels? 3. What about accounting for the background fluorescence?

[@souheilasemache6142]

Excellent video, how can I use imagej to measures crack width and opening and distance between cracks a renforced concrete beam? Please help me, thanks you

[@blancadesalvador4201]

HI!! COULD YOU TELL ME THE UNITS THAT THE NUMBERS THAT APPEAR ARE? THANKSSS!! :)

[@muhammadsohial157]

Could you teach me on ZOOM as i am beginner and can not make a graph for immunoflourescence intesity

[@aathirahvalmaypriyono6954]

hey, thanks for sharing! do you know what is the maximum amount of the intensity? thank you!

[@turkdogt6251]

This video was not helpful to me at all, plus you have a lot of background noise that you have to clear up in that picture. You do not show a graph, a plot or anything. This video is useless. If anyone needs to see a on plotting intensity look at "Mrs Alicerita" video she goes into detail, shows you tips and tricks and shows you how to build the actual graph not this nonsense