🔬 How to find out if the microscope objective is dirty | Amateur Microscopy

What to do when you do not see anything with the microscope. Maybe the objective is dirty, but how can you find out?
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[@maha1maha777]

Hi, thank you for all work One method to see the front lens of a microscope objective is to check it with a stereo microscope if you have one Another way I find, is to illuminate the lens from the back with a laser and see the image projected on a white clean wall, work most with 60 and 100x lenses, rotate the lens to identify which patterns correspond to the microscope objective, laser lens will have also some dust on it and you will see it on wall. Do not look directly with your eyes thru the microscope lens with laser on, use projection on the wall, usually at 1.5m distance from the wall the laser will make approximate 2m diameter image of the lens. Shure the image will over impose all lenses surfaces of the objective (you will se even the phase ring).

[@The_Horizon]

This greenscreen is actually very good, I didn't even realize till half way through

[@rubenremus5649]

Another possibility to check out is to make sure that the focus limiter is not set to a position that prevents bringing the high power objectives to focus.

[@jamesaverill6946]

Checking the front lens of a 40x objective is easily accomplished by removing one of your oculars (10x) and inverting it (handheld) as you would a loupe. Looking through the front lens of your ocular you want to get in close to the front lens of the objective. Now you will now see any immersion oil that may need cleaning. We have also seen 40x objectives hitting the mounting media of freshly made teaching or clinical slides.....too soon before it dries! Big problem to clean but it can be done. Also we encounter objectives that have delaminated (inside) due to age as well as those that are dropped on the floor (front lens takes the blow)...get out your wallet for a new objective. They cannot be repaired economically. The responsible culprit will replace the objective on the nosepiece and wait for the next user to discover the mysterious problem.

[@BTNE_Videos]

Very good explanation, I agree that most common problem is contamination by immersion oil, I made this mistake yesterday, moving my 20x right into the oil!

[@neilthomas649]

Thank you very much for very informative videos.

[@dorsetdumpling5387]

Oliver, your videos are always top quality and you give yourself more work to do with a newsletter?! Really appreciate your efforts - many thanks.

[@MicrobehunterMicroscopy]

Thank you for the nice feedback. This is possible because I have now a certain routine in filming and video editing, so it has become manageable now. And I do enjoy it, so it does not feel like much work!

[@beblessed1030]

really some solid contents appreciate man

[@eddieraru7054]

Nice informative show mate...

[@macncheese8686]

I also had this problem. Thanks!

[@dr.udayanbhattacharya1107]

I have a question. Which objective of 45x is better spring loaded or non spring loaded (fixed)?

[@abdulkadirabdirasak3644]

Thanks, microbehunter for your helping me and got much better on my Qu.

[@Raghavendrasugandhi84]

Hi sir...i m using 10X eyepiece and 45X objective...i have tried all possible way by changing the distance between specimen and objective and the distance between eyepiece and objective...image is blur...plz help

[@Zonniezonnie_A]

In my 100 there are tiny little dots like almost looks like tiny little water dots but they're all in uniform like it's not sporadic. It's all together and it won't come off. I don't know what that is.

[@leoescamilla5497]

I once thought my 40x objective was not working/was damaged. It turns out, I just put the slide upside down. I couldn't see the sample with it because the glass was too thick.

[@ardaarya8499]

I had the same problem with 100x objective. Just replace the upper glass with a 0.1m thickness glass

[@wemcal]

Great information

[@Microbehunter]

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[@wackyvorlon]

I was wondering, how often do you need to clean your eyepieces?

[@FLODDI100]

He already made a video for that. I think it's called "How to clean eyepieces and objectives" or something like that. There is no actual "how often". If it gets dirty because of your eyelashes or something like that you should remove it carefully. Dont take it out or sth. like that except you see dust after the surface cleaning. If its clean you risk to get dust or other particles in your microscope which will make your life much harder :) Hopefully this was useful for you. If you want to know how to clean it, watch his video :)

[@eddieraru7054]

Every time you use your dick you must wash off the lubricants same to lens. Every one use clean it up.

[@eddieraru7054]

Common senses is the stand post when you come to what decision you have to make mate.

[@eddieraru7054]

Cleanliness is next to goodness, the better it is the best result your work done is best satisfied.

[@SushantMore]

sir i want to fit my NIKON to my stereo zoom microscope please help where I can get an adapter like you which is present in this video

[@MicrobehunterMicroscopy]

Watch this: kzbin.info/www/bejne/gKabo5qbf9dmmKs The adapter in the video is an authentic Olympus adapter, with projection ocular. Expensive, rare, good, but not necessary. On Amazon they sell a range of DSLR microscope adapters that are much smaller.

[@brandongilbert6488]

Thanks very usefull