How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus

In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media such as mTeSR™1 into mTeSR™ Plus. This video will cover the generation of cell aggregates and passaging protocol.
0:00 - Intro
1:23 - ReLeSR™ Passaging
3:14 - GCDR Passaging
5:56 - Plate Setup for mTeSR Plus Transition
Watch the other videos in the series below:
Video 1: Plate Coating - • How to Coat Plates for...
Video 3: Maintenance and Morphological Assessment - • How to Maintain and As...
Learn more about mTeSR™ Plus hPSC maintenance media: bit.ly/3l2qJKt
View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus: bit.ly/3etrGJm
#PluripotentStemCells #CellCulture #mTeSRPlus
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[@Ca-yj8wj]

Hello, is it possible to use PBS instead of D-PBS?

[@STEMCELLTechnologies]

Hi, there is no significant difference between PBS and DPBS. Both of them contain sodium phosphate, sodium chloride, and, when required, potassium phosphate and potassium chloride. Other preparations, such as DPBS, may or may not contain calcium and magnesium. PBS and DPBS have numerous applications because they are not noxious to cells. Please make sure however that you are using a formulation without calcium and magnesium when generating cell aggregates and passaging hPSC in mTeSR™ Plus. If you have any more questions, feel free to email us at [e-mail].

[@alberpajares4792]

Yeah you can increase the power of your cells getting used to do difficult things,..

[@alberpajares4792]

Each time you solve a logical problem you create new brain connections,.. same when you complete a physical challenge for your own achievement,..