Introduction to cell culture, splitting cells using trypsin and counting them using a hemocytometer

This video provides you with a general overview of the procedures typically used to "spit" a culture of immortalized adherent human cells maintained in tissue culture in a T75 flask.

[@ady12377]

This helped me alot! Thank you maam ! ❤ may you live a long and meaningful life ❤❤❤😊

[@bariatricbabe7438]

This professor was my favorite professor and made the greatest impact on my life. I really wouldn't be the scientist I am today without his guidance and teaching.

[@user-ur7gk8st3x]

Hello can you plss tell me application of immunohistochemistry

[@funny11744]

Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?

[@virgirma1328_PhD_scholar]

Very thorough tutorial !Thanks teacher !

[@Lunedai]

I am so greatful for this helpful video! Thank you so much

[@paolarosado8348]

Great video and explanations! Thank you for uploading it. I really enjoyed the camera technique, because is like you are doing it yourself.

[@pichej22]

TYSM for making this video. I'm currently applying for entry-level positions in biotech and this is one of the most sought-after techniques. This video is helping me identify the similarities between the cell culture I do now mammalian.

[@MonikaShailesh777]

Thank u Olga 💓...keep posting, really helpful video 😊

[@alessiadauria1490]

Great video, thanks! Very helpul for my first times in the lab

[@biologylover1565]

Amazing video, Thank you for your effort. 👍👍👍

[@Koonnuusana]

Thank you so much for this good video. It make me clearly understand about cell culture. 😊❤🍀👩‍🔬

[@mdselimahamed8617]

clear explanation indeed .

[@tseringsamkhar1198]

Very insightful . Thank u

[@alissaa.2287]

You are a hero to me, thank you.

[@jacqueonassismec3430]

Thank you very much for this teaching..

[@zuhayousuf9197]

Such a good video! Thank you :)

[@ko-fanta9829]

well prepared and clear

[@hallahadi5548]

braaaaaava you are the best one who was explanation cell culture to me >>> thanks a lot

Happy to hear that!

[@JYOtiRaNJanMANgaRaj]

Thank you so much 🙏🙏🙏❤❤❤❤❤❤❤❤❤❤

[@shutterismmn]

this video made my concept clear. thanks

You're welcome!

[@aydafathollahpour602]

Thank you, it was amazing

[@curiouscreature747]

thanks olga soto

[@fallseason5163]

Super super thank you for sharing it with us… do you have experience with BREAST CANCER STEM CELLS???

[@anoopyes5004]

Tysm for the video❤

[@ruqaiyatasneem8594]

What about renoving the neutralized trypsin and adding fresh media ?

[@niloofarkh4779]

THANK U SO MUCH

[@funny11744]

Please mention how to count and distinguish ADSC ( Adipos derived stem cell) from other cells from SVF , using HEMOCYTOMETER and microscope. What total magnification of microscope îs required ? Thanks

[@sainaboujatta3108]

helpful!

[@osmirod1]

Now that you have the number of cells per whatever volume (mL), how do you calculate the correct volume to seed a desired number of cells?

[@mxasf]

Nice video, my only recommendation is to wash the cells with dPBS (lacking Mg and Ca salts) rather than PBS prior to trypsin. Those divalent cations inhibit/slow down trypsin. I always wash at least two, sometimes three times, to speed up the trypsin step, which sometimes takes like 20min for my weird cells. Is It is a super cheap reagent, so not an issue to be wasteful.

Great point!

[@livinghope8039]

great job, I liked and sub.. it

[@SweetMangoMuncher]

More trypsin may be necessary depending on the potency of the trypsin as well

[@kosheeka]

Cell detachment with trypsin depends on its potency - more active enzymes per volume means less solution is needed. To minimize cell damage, use the lowest amount of a high potency trypsin solution that effectively detaches your specific cell line.

[@dhivyadharshiniboopalan2069]

What is the name of that aspirator used in discarding ?

[@monaelrefay2956]

Do you make other videos for MTT assay

[@samratpaul25]

Are any animals killed during the extraction of Trypsin from their pancreas ? Or can it be done without killing the animal ?

There are two main methods to produce the trypsin used in tissue culture. One is by purifying it from the pancreas of pigs; those pigs are killed by the thousands for human consumption, so in a way this uses a part of the animal usually considered of no value. The other method is by expressing it in recombinant expression systems. In this alternative method, no animal byproducts are used.

[@amitshivhare6690]

A̺m̺a̺z̺i̺n̺g̺ v̺i̺d̺e̺o̺ ❤

[@funny11744]

At the minute 0:47 it is written ,, Immortalized cells could ....indefinetely" . Somebody could explain ?

[@handydan6273]

See I'm wondering if it's yet possible to split two cells from two different species and combine them with success? There is a cell that eats away at plastic. Could it be possible to combine it with another cell or organism?

[@getoverit2800]

every time i see someone with bare skin (like the arm not covered by the lab coat or the gloves) inside the BSC i die a little bit inside. especially when they do it while talking about aseptic practice.