How to Make Ethanol (Fermentation)

This is the first of two videos on how to ferment and distill ethanol at home. Ethanol is used as a chemical solvent and is very useful in many laboratory reactions. As well, I will be using it to extract fragile oils and act as a carrier for my perfumes. This video shows how to set up for a sugar fermentation of ethanol and the next video will show me distilling off and further purifying the ethanol with molecular seives.

[@knuckle47]

I’m looking forward to your next video...great production and great information

[@thebiomajor5750]

luthier47 Thank you! I started the channel for myself and so i don’t have a lot of material or ideas of what people would like to see but its nice to hear that other people enjoy what Ive put together so far. Thanks for your comment. :)

[@said.skopal]

That what you make its not pure etanol.How to extract etanol from that?

[@axelvauthier6823]

​@@thebiomajor5750esque ont peut l utiliser dans les voitures essence bmw e46 330i touring 2001 sans modif et 406 break

[@wannabefarmgirl9595]

Great information Bio Major! Keep em coming! 💕

[@abdellahgogop5653]

Can i the same yeast that is used for bread elevation??? It is Saccharomyces serevicieae.

[@aymanelrafee1]

Thanks dear forthat video - i did that at home but the alchol has a yeast odour - how can i get rid of that odour ?

[@thebiomajor5750]

انا احب الفيزياء the more times it is distilled, the less of that there will be.

[@SensiStarToaster]

Also filtration through activated carbon works well.

[@aminilabja]

Can we use it for preparing hand sanitizer???

[@sorena.pn.s4690]

Hello. What are the exact amounts of sugar and yeast per liter of water to produce ethanol alcohol? 《 Thank you 》 Please answer my question immediately. Thanks

[@sreehari9208]

What will happen when we add 500g sugar in 1lit water and yeast

[@thebiomajor5750]

Sree Hari Haven’t tried that. Why don’t you try and report back how well it works?

[@Adamczuk335]

too much sugar. the yeast would probably die by 8%, and there may also be no fermentation

[@2gr_t95]

hello, doesn't heating the solution before adding the yeasts help with the reaction, as we need the yeasts to perform anaerobic respiration ?

[@joshwilson9393]

I‘m not sure what good that would do, but I‘d be worried about killing the yeast with heat.

[@2gr_t95]

@@joshwilson9393 let me explain here : by heating beforehand the water, there's less O2 dissolved in the water so, if the yeast is already activated, by heating water and letting it cool down (with no contact with the outside air) and then add the yeast, i think you can shorten your reaction time as the yeast will only need to perform the synthesis of ethanol, and not "eat" all the O2 in the water and the jar

[@joshwilson9393]

@@2gr_t95 that’s smart! It seems like it would work theoretically.

[@SensiStarToaster]

I dont think that is the reason why first booling a highly concentrated sucrose solution speeds up fermentation. There are 2 benefits to first boiling a saturated sucrose syrup solution prior to dilution and addition of yeast. 1. It sterilizes the sugar so yeast will not compete with bacteria while fermenting). 2. Yeast has a strong preference for glucose over fructose as a substrate. By boiling for an extended period (particularly if the solution is firstly slightly acidified e.g with citric acid) hydrolysis of sucrose into glucose and fructose monomers occurs. This greatly speads up the rate of fermentation since the yeast can directly metabolize the glucose preferentially. There will still be a significant quantity of fructose in solution by the time ethanol concentrations rises to a level toxic to the yeast itself. If the end goal is ethanol production this saves a lot of time. It's true that fermentation requires an anaerobic environment (otherwise you get lactic acid instead of ethanol, the amount of 02 in the water is negligible if fermentation occurs under an air lock and is consumed very rapidly very early on. So this factor can be safely ignored without any noticeable effect on yield.

[@naionkabir1603]

Please name some of the equipments used to manufacture alcohol here

[@Anjalikargeti]

Hello sir I'm a bachelor's research student. Sir i will be grateful if you help me. can you please tell me how separate ethanol from fermented mesh (agroindustrial pretreated). Pls sir reply 🙏🏻🙇‍♀️

[@Vizmaximoff]

Won't filtration solve this??

[@taliagreenberg5228]

Thank you .is this the ethanol alchohol that is used in art projects?

[@thebiomajor5750]

Ethyl alcohol or Ethanol is the alcohol found in beer, wine, etc. But it's also used in hand sanitizers such as 3M's Avagard. What kind of art projects call for alcohol?

[@taliagreenberg5228]

@@thebiomajor5750 ethanol or alchohol 99% is used to create different effects with acrylic or alchohol inks...check out Gerda Lipski's work with ethanol/alchohol ..

[@MoneyForFun255]

2 questions! Can i use bread yeast and can i drink this if i mix it with water?

[@carl3693]

I'm pretty sure you can use cooking yeast, but you'll prolly just need more

[@JagexAnarchy]

Thanks for video, what is the alcohol % after 21 day sugar wash fermentation?

[@thebiomajor5750]

Dillon thanks for the comment. :) Directly after fermentation the ethanol isn’t particularly high. I think it was around 60-70% The link in the description shows me distilling it though and you can see what the hydrometer’s at in that one.

[@JeremyChung]

@@thebiomajor5750 Hey man, I'm doing fermentation/distillation for a science project. Isn't it only about 15% after fermentation?

[@zapid6733]

@@JeremyChung Yeah 60-70% is impossible from just fermenting, the yeasts would die way before they hit that %.

[@kartikchoudhary4342]

What is that airlock? Please help

[@martinbisschoff988]

To assist with carbon dioxide escape from the glass container and prevent oxygen from entering.

[@shayanshokoohi3337]

thats great- i want produce ethanol with glucose, for example glucose with berix 37, but i dont know how much water to this solution and how much yeast. can help me anyone?

[@johnsmack5253]

How mnay lb of sugar per gallon of water ?

[@mgmadani1]

Hi, the is the composition of the yeast available in my market, (_Saccharomyces cerevisiae , hydrating agent sorbitan monostereate E491_), will it work to make alcohol ...thanks for your help

[@thebiomajor5750]

M G yes that organism will produce ethanol and is often used in wine and beer making. :)

[@ranjanty]

Can this alcohol be used for extracting mushrooms?

[@JohnKnowles-fm3sq]

Yes

[@ellyme928]

Good day @Bio Major ! I am interested about this video and I am looking forward to have contant with you. Hope you read this.

[@ygwy87]

Good invention

[@fake-chemistry]

can we drink it.

[@trongdung1306]

Are you using sucrose or glucose?

[@lokeshdarling1766]

Ingredients 10l water, 5 kg sugar, and 25 grams active yeast. This is good ?, fermentation 3days.

[@sorena.pn.s4690]

Hello. I use ethanol alcohol for disinfection. And according to your order, I used 50 kg of sugar and 250 g of yeast (of course baking yeast) in 100 liters of distilled water. Now the question is: 1_At the end of the fermentation period, what is the percentage of fermented alcohol? 2. How many days does the fermentation period last? Thank you very much. Please guide me.👍🙏❤💜🌹

[@kevincosmicomusic]

How sterile does this set up have to be?

[@joshwilson9393]

The more, the better but a simple diluted bleach solution did the trick before I bottled everything up.

[@xdgaming952]

seems cool

[@White.Kaiser]

What type of yeast do you use

[@thebiomajor5750]

Bjørn I used Lavin K8 brewers yeast.

[@White.Kaiser]

kemisola Ehindero how is this even relevant to the subject?

[@CatDaddySteve]

Kilograms , are You from Europe, you sound American

[@Southghost5997]

Warming is not necessary Yeast also need nutrients, but most brewing yeast sachets include this. It's good enough for 4 gallons. You dont need 21 days, more like 2 days. If it takes longer, its too cold or you've done something else wrong.

[@lokeshdarling1766]

How much come from 10 l sugar wash to alcohol. Vodka

[@unvaccinatedAndPureBlood]

How to make ethanol from algae?

[@Yoki_Hansi]

I need reagents

[@فرجاللهحنفيحمودة]

هل يصلح للشرب

[@私の叱責ムハンマド]

المعلم فرج فرج اذا شربته بتموت انتبه 😂🍻

[@MrBugman2525]

I want to use it for hand sanitizer

[@thebiomajor5750]

Mike Burns it should work for that. The sanitizer we use at the hospital is around 65% ethanol.

[@私の叱責ムハンマド]

well done

[@thebiomajor5750]

thanks!

[@bertsquared2631]

how much concentration?

[@thebiomajor5750]

Robert John Evangelista using repeated distillations preferably with a fractionating column, and afterwards soaking up excess water with molecular sieves, it is possible to get the concentration to 100%

[@EdwardTriesToScience]

18% then the yeast dies.

[@sai678678]

Can we drink. mix with coca cola or etc...

[@thebiomajor5750]

sai Not unless you have a very good way to determine purity.

[@berrycodec2216]

Why u want drink with coca cola??

[@wormhole331]

Don't use distilled water. Yeast need some minerals and will do better if they have some other nutrients.

[@thebiomajor5750]

But if you don't, how do you prevent other microbes from getting in and spoiling the batch?

[@bartek6100]

@@thebiomajor5750 you can boil water before

[@extraryc7316]

How to make ethanol using orange peel?

[@wumbology3109]

You cant

[@wumbology3109]

I'm pretty sure you can turn it into oil called limonene which is flamable just like ethanol

[@thebiomajor5750]

@@wumbology3109 you can indeed extract limonene from the peels. I actually have a video doing that, well at least extracting the essential oil which contains limonene.

[@wloffy]

we are not the same u making ethanol for experiments i make ethanol for drinking

[@ابنسوريةالثورة]

ممكن الترجمة بلعربي مع المقادير 👍🌷

[@thebiomajor5750]

ابن سورية الثورة wish i spoke whatever language this is. :) Arabic

[@ابنسوريةالثورة]

@@thebiomajor5750 😢

[@mohammeda.raqeeb3110]

٢ كيلوغرام سكر قارورة تخمير كبيرة بمغلق غازي عبوتين من خميرة KA برو حوض للخلط ومقياس للسوائل وميزان اهم خطوة تحدث عنها هو التاكد من ان الخميرة جيدة وفعالة وذلك باضافة الخميرة الى كوب من السكر وتركها ل ١٥ دقيقة اذا ظهر ثاني اوكسيد الكربون(على هيئة رغوة كثيرة كما هو بالفيديو" فيعني انه فعال. باقي الفيديو واضح

[@mohammeda.raqeeb3110]

@@thebiomajor5750 Yes, it is Arabic. He asked for a translation for the ingredients and volume "proportions".

[@ابنسوريةالثورة]

@@mohammeda.raqeeb3110 مشكور اكتير 👍😍

[@yemenigcse1]

re edition your video needs

[@thebiomajor5750]

I need to make more videos. Just haven’t had much time with school.

[@yemenigcse1]

@@thebiomajor5750 how i can help

[@私の叱責ムハンマド]

انتبهو هذه كحول طبيه ✋🏻

[@wumbology3109]

English pls

[@funtimes9196]

So do you get drunk drinking this?

[@yayayayya4731]

No. It can have methanol which can kill you.

[@lokeshdarling1766]

Please give me reply

[@thebiomajor5750]

I added a link to another video I made which shows the amounts in the description.

[@nateramsbacher992]

Copied nile red