How to Make Liquid Culture | Liquid Culture Lids | Take a Live Culture | Inoculate a Spawn Bag

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In this video you will learn how to make liquid culture, liquid culture lids, take a live culture, test your liquid culture for contamination, and inoculate a spawn bag with liquid culture.
0:00 Intro
0:19 Liquid Culture Lids
1:38 How to Make Liquid Culture
3:53 How to a Take a Live Culture
7:44 How to Test Liquid Culture for Contamination
10:28 How to Inoculate a Spawn Bag with Liquid Culture
13:01 Check out my new Channel Ashley Shroomer!
Liquid Culture Media - www.shroomsupply.com/agar-cul...
Rubber Gasket for Jar Lids (90mm Widemouth) (You will need this in order to get a tight seal on the lid after you sterilize. I did not use this in my videos but I should have. I will make an updated video when my gaskets get delivered) - www.shroomsupply.com/lab-supp...
PP5 Plastic Lids (90 mm - Wide Mouth) - www.shroomsupply.com/myco-jar...
3 Inch Liquid Culture Needle - www.shroomsupply.com/syringes...
12 cc Syringe - www.shroomsupply.com/syringes...
Red Self-healing Injection Port - www.shroomsupply.com/lab-supp...
Use a 1/2” Forstner Drill Bit - amzn.to/39haXI5
Gas Exchange Filter (13mm Diameter) - www.shroomsupply.com/air-filt...
Use a 5/32” Drill Bit - amzn.to/3ruKSeZ
FREE Mushroom Growing Guide!
boomershroomer.com/beginner-m...
Monotub Dry Bulk Substrate Ingredients Kit
www.etsy.com/listing/94207271...
Inflatable Monotubs for Sale!
boomershroomer.com/monotub/
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32 Oz Wide Mouth Mason Jars - amzn.to/3d37UnT (They are cheaper at Wal-Mart or Target but just wanted to provide a link. Also I read a lot of the reviews and they often break in shipping, better to pick them up at your local store.)
Hamilton Beach Food Dehydrator - amzn.to/2YigCHI
Coco Coir - amzn.to/2YHX3cn
Vermiculite - amzn.to/3hIPtH4
Gypsum - amzn.to/2XdbmEV
Petri dishes - amzn.to/3niKLRw
Presto Pressure cooker - amzn.to/3hMngPv
Humidifier - amzn.to/3hNdM6H
Humidity Controller - amzn.to/3hSjfcn
Timer - amzn.to/2Xfl5L2
Fan with speed controller - amzn.to/3blqicn
Heating Pad with Thermostat - amzn.to/39rgGKu
Spray Bottle - amzn.to/3s3pAGD
Black Plastic Liner Material - amzn.to/39qrJn4
Sterile Disposable Scalpes - amzn.to/3qmcwKN
Isopropyl Alcohol, 70% - amzn.to/3pop6JB
Alcohol Prep Pads - amzn.to/2YieAr4
Indoor Grow Lights - amzn.to/3qXuFyR
Polyfil - amzn.to/2MaydPp
My Camera Equipment
GoPro 7 (Timelapse) - amzn.to/3pt6aco
Nikon D5600 (Timelapse) - amzn.to/3t7KhSf
Canon 80D (Main Front Camera) - amzn.to/31M2WGE
Venus Optics Laowa (Macro Probe Lens) - amzn.to/3sMMtxP
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[@jesush.christ5978]

You don't need an injection port. put two layers of foil on the top of your jar. Lift up the first layer and inject culture or spore into the jar. It's been working fine for me for years.

[@trump45and2zig-zags]

Any other tips for anewbie? Spores n all in 1 grow bag should be here this week

[@jesush.christ5978]

@@trump45and2zig-zags make a still air box. Its hard to explain, you get a tote and make two holes for your hand and get some pvc pipe or something your hand can fit through. Glue some of those big rubber cleaning glove to the inside and seal everything up. I put glade wrap over the top of mine so I can see better. Put everything you need in, and spray some rubbing alcohol in, then put the wrap over it and let it sit a while.

[@trump45and2zig-zags]

@@jesush.christ5978 like a sandblaster, I gotcha, thanks. Excited to grow my first batch when I get everything this week

[@trump45and2zig-zags]

N you pretty much just make a foil pouch with the jar, right?

[@jesush.christ5978]

@@trump45and2zig-zags are you using jars and doing the brown rice flour tek?

[@donivanhummel3106]

Thank you, as a person who is new to mushroom cultivation, I appreciate how step by step you are with your videos, you're an excellent and thorough teacher.

[@BoomerShroomer]

thank you!

[@zoghunter82318]

Pro tip for anyone doing this for the first time, always run your drill in reverse when drilling plastics to prevent cracking.

[@zoghunter82318]

@Valerian Roots Yup, if you're drilling plastic, no matter the size of the hole, run the drill in reverse and don't push to hard. Works every time.

[@seanweber8993]

My guy you are my hero. I’m starting up my company and trying to get all aspects for sale, including culture lids. Went to mass produce them and about had a stroke from how frustrated I was getting from the lids cracking. You are a god.

[@FLORIDAMANIAM-he2lz]

Pro tip. Use a one of those triangle stone bits and drill through like you would a step bit. Its just sand through so there is no risk of craking or breaking at all

[@madmaxofspokane1691]

Or use a drill & brad point bits...

[@fun_ghoul]

I don't understand this comment. With no special effort, I've never cracked a plastic mason jar by drilling. Frankly, if I was doing a shotload (ahem) of these, I'd probably pop for a drill press and do 10 at a time.

[@jasonnascimento9174]

I never comment but I want to thank you for the way you instruct. There are many out there but I keep coming back to your info because it is basic in the best way possible. Sometimes it gets so confusing. Your instructions are very linear. Thanks.

[@TutorialesdeNaraCarolina]

Gracias Ashley. Muy clara y didáctica tu explicación. Me diste mucha información útil!

[@rattlesnakenate8732]

This is probably the best instructional video I've ever seen!!!! Thank you!!

[@stevenscrafting2467]

If you forgot the aluminum foil also I found a really good way of keeping the coffee filters over the jars by using the original ball rim the The jar came with Nice deep and snug

[@semmett22]

Where do you store your liquid culture jars, A cool dark place, a Fridge…? Also how long will a jar last before it’s no longer good to use? Btw, Thanks Ashley for another perfectly narrated video!!🫶🏼

[@joey_btw4718]

Wow! Thank you! This was by far the most thorough step by step video I've found so far, and I've watched probably a dozen or more

[@Bootysmoothie]

I love the way you hold the jar to get good angles on the pieces, you can see the growth off tot the side of the first little lump of mush

[@guymontag2948]

I bought the HaBeuniver 20mm injection ports, the shorter grey ones that seem to be very common, and they'll fall right out of a 1/2" hole but a 7/16" is a perfect fit. Thought this might save someone buying the wrong bit. A brad point bit works great on the standard metal lids, btw. Great video, Ashley, as always.

[@addisondraper3574]

The best video on the subject that I've seen. Thank you.

[@BoomerShroomer]

Wow, thank you! I appreciate it. 🍄❤️🍄

[@naftalibendavid]

Always grateful for the info as well as your optimistic attitude

[@BoomerShroomer]

Thank you for your kind words! :-)

[@robinhoode9364]

Very informative and well-made video. Top Quality! Thank you.

[@naturallydope247]

Great video! Loved it. This was one of the better explained ones.

[@BoomerShroomer]

Glad it was helpful!

[@MrEiht]

Thank you so much for the footage of the development. I put Lion's mane LC in such a jar to let it grow. After a week I only see a tiny bit. But I asked smart people and they said it is alive. But way slower than the life you showed there. Good luck with your work!!!

[@tokkichainsaw2371]

this is the best tutorial so far! thank you so much!!!

[@soccer2themax]

Love your technique and explaining style. Very good

[@BoomerShroomer]

Thank you so much!

[@ducpham2007]

Even your voice, so good videos lady !keep them up !

[@JedF2010]

It's great that you put links to everything used. Also nice to see info on your camera kit. Great vid!

[@BoomerShroomer]

Thank you!

[@DanaTS]

Great video. Thank you! It’s very helpful.

[@BoomerShroomer]

Glad it was helpful! Thank you!

[@motorheadmaximus]

As useful and fun to watch as always, thank you !

[@BoomerShroomer]

Thank you.

[@jameskreiter]

Ashley, you are AWESOME!!! I have asked you two questions, and you have answered them both. Unlike Cameron the Grain Man where I bought some grain, he did not answer me on YT or via email. And guess what, he sold me bad grain, did not spawn a bloody thing. So big huge thanks to you, what your are sharing, and doing. What you are sharing is invaluable to all of us Mycology Students.

[@BoomerShroomer]

Thank you. Did Cameron replace your bags? How many were bad?

[@jameskreiter]

@@BoomerShroomer I ordered five, the first was bad. I lost one bag, and the spores. I have four left, and there is no way I will try them. That is $50 down the tubes.

[@BoomerShroomer]

@@jameskreiter how do the other ones look? As long as they smell good and you can’t see contamination they should be good to use. I use his bags lot and rarely have issues.

[@BoomerShroomer]

@@jameskreiter I’d be happy to refund you your order from them.

[@jameskreiter]

@@BoomerShroomer They all look the same. and smelled just fine. I am afraid to even try them. Moving forward I am doing the whole process in house. At least I only have me to blame.

[@markrawson1435]

thanks for the links, makes it so easy, love it

[@nexusnostalgia]

Love that you use unbleached filters!

[@BoomerShroomer]

Thank you!

[@sonotuttotuo7782]

Thank you immensely. I like your video. As a beginner. I have to watch it over and over again.

[@icy7136]

THANKS ASHLEY! AWESOME VID!

[@mealbla7097]

So thorough, thank you

[@joepangit6938]

Amazon has those lids in colors, with silicone gaskets. They are sweet. I use 1/4 drill grey injection ports, and 1/2" stick on air exchange glued down with red high temp RTV sealer. They work great!

[@ohiosu55]

What’s the lids called?

[@TheShoottheradio]

Is that an injection Port filter? I have a question about injection ports when you tip the jar over doesn't the filter get wet?

[@tyeejoseph3358]

Thank you for the educational content. Love from Canada :)

[@BoomerShroomer]

Thank you!! 😊

[@doinksinthePM]

10:45 it's also the perfect time to 'noc up a few more jars with that LC so you can save some time on turnaround. Instead of having to go from the tissue biopsy stage next time. Lovely video BTW! Makes me want to get growing again. So much faster than cannabis with zero electricity required and basically one brief moment of light just to start pinning? You can't beat that. I'm just super weary about trying to go myco-psycho again because the last go around I got arrested for unrelated reasons and wasn't able to return home for a few weeks. By that time I had 8 Rubbermaid tubs that were just an orgiastic death feast of aspergillus and black mold plus some contams I'd never even seen before. Ever since then I can't even keep brand new bread in this freaking apartment for 2 days without it molding. I'm terrified if I put new tubs in they'd have the same exact fate.

[@rartucio]

By doing the tissue biopsy you select the best genetics from the mycelium. A pity to hear what happend. Anyways if you esterilize properly Im sure your not going to have any problem.

[@mcfats7652]

You're going to need fungicide spray. Look it up, set up more than one in your case. Perhaps everywhere you put tubs in the past as well as one for the common areas. Set the sprayer up and leave the apartment for the day. Come back and you should be in better shape.

[@chrisgrassi4161]

Thank you so much for sharing your knowledge. 🙌

[@efvenergyfrequencyvibratio5231]

Thanks Ashley!

[@BoomerShroomer]

Thank you!

[@gnarmarmilla]

Hey Ashley, check this out, this is from a fellow named Marc R. Keith who has been growing since the 70’s, “Throwing spores into honey or karo has to be the worst possible way to make a liquid culture and I'll be glad when this current fad passes and folks can get back to established techniques. Just inoculate some grains or brf in the standard way and you'll be miles ahead. For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores. Upon full colonization, shake the jar to loosen each individual kernel. Be sure to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc. In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains. 3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out. You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes. Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this. Remember, mycelium grows poorly in water unless under constant stir which oxygenates it. With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels. LIQUID CULTURE - A glovebox is not advanced equipment. One can be made free with stuff around the house, so there's no need for people to work in open air or fecal and mold infested bathrooms and kitchens. There is also no way to generate 100 full syringes of liquid mycelium in very small pieces within two weeks from injecting spores into honey or karo. I'm sure if people blindly used the grain method I described above, many would still have problems too, because they're inserting unproved and possibly contaminated spore solution into grains, which also isn't proper mycological technique. However, agar can easily be poured in a glovebox, and the Petri dishes used to isolate healthy mushroom mycelium away from any contaminants in less time than it takes to even know if a karo LC is contaminated or not. That agar wedge can then be used to inoculate the quart(or pint) jar of grains, that can then be turned into at least 100 syringes, or used for g2g transfer to get ten jars the old standard way. In addition, after the water is poured out of the grain jar, it can be placed back on the shelf to re-colonize, and the process can be repeated again. I just strongly feel new growers are doing themselves a disservice when they simply inject spores into honey water and then sit there waiting for something to happen. At the very least, inject directly to grains so you can visually inspect the process. Perhaps Agar's lids with injection ports will help those who have never used grains to get off to a good start.” From, “All of RR’s notes.”

[@TheJavirich]

Such a complete video thank you so much

[@BoomerShroomer]

Thank you!

[@slocoast5]

so easy everyone can do this :) thanks!

[@amanglazo9283]

easy to follow thank you.

[@erndog64]

Great video!!! Thank you!!

[@BoomerShroomer]

You are so welcome!

[@theleopardsociety4845]

So professional!

[@eileeniawphniaw8981]

Thank u for your kind of educate culture.

[@BoomerShroomer]

Thank you!

[@poppyjones1892]

Very nice personality , something feels relaxing and refreshing they way she teaches 😊

[@alum798]

hi , really like your videos just wondering how and how long can you store your bottle of liquid culture

[@ericweaver1693]

Thanks!

[@NikuKashi]

05:05 Is this method better than agar? (raw/fresh shroom into agar)

[@johnwinger7504]

Great teacher!

[@panda-gy4ni]

What size needle is that?

[@williamclarke2245]

Thank you !

[@hhyy3173]

Great video thank you ❤️

[@BoomerShroomer]

Thanks for watching! ❤️

[@donkulasmushroomsandagri3123]

Love it big thanks Lods 🍄🍄👍

[@ivanangelototola2472]

Bem detalhado. Excelente.

[@harryfare4396]

I bought the half inch Forester drill bit and it works great it doesn't leave those nasty burrs

[@Chimmers69]

Informative vid, thanks!

[@BoomerShroomer]

Glad it was helpful! Thank you!

[@yosig6659]

love your vids keep it up!!!

[@BoomerShroomer]

Thanks, will do!

[@BoomerShroomer]

@@grandpamosses1224 thank you!

[@jorgerusso2117]

Hi there.. great video, very detailed.. One quick question tho, what needle gauge are you using. I used regular needles and the mycellium doesnt go through the needle, it stuck to the tip. Thank you!!!

[@karlkramer7687]

Any tips for making a flow hood? Where do you get supplies? Thanks!

[@Trenislife]

Another fantastic video! Very well presented and easy to learn from. I will definitely be giving this a go in the near future.

[@BoomerShroomer]

Awesome, thank you!

[@chuckcharlebois4199]

Ashley. You’re the best. Love your channel. What gauge and length of syringes are you using. Mine seem small and these look best for liquid culture

[@Trenislife]

@@chuckcharlebois4199 use draw up needles

[@MerrillBrown]

Hi Ashley great video I’m about to do my first LC project. Please tell me where you got your syringe and looks like a biopsy needle. Thank you

[@hoseaahadi4889]

Hi madam Ashley,thanks for your video I have lent especially the way you find messenger and then innoculate to dishes

[@kiya1104]

It was Great, Thanks

[@CodeCancerLab]

Brilliant video well explained 😁

[@BoomerShroomer]

Glad you liked it!

[@depravedregime5288]

Thanks Ash ✌️

[@heroesrevival1164]

great video great channel thanks

[@masacatior]

Knowing you can grow LC from biopsy instead of spores helped me a lot! Thank you.

[@dougreynolds2813]

what temp for LC and dishes? good video, i learned a lot; it gave me confidence, Thank You!

[@heywoodjahblomie325]

Hey Ashley what gauge needle do you use to extract the LC then of course, inoculate the fresh grain jar please? I see blunt and Sharpe needles Thanks.

[@nikoventuracorleone]

Thnx my Darling

[@SchnellgelerntDE]

Really good work i had many troubles with my spawn bags... because it was always too hot, i think ur doint this since a while xD

[@heatherrichart-bishop5412]

This is really good sister. I like all the comments as well. Truly- I've been looking for the right videos.

[@BoomerShroomer]

I'm so glad! Thank you!

[@Chris-dy5fy]

Liquid culture is definitely the way to go.

[@Bootysmoothie]

I took morel culture possibly correctly, the matter that has formed is the same shade of blue Grey and the bases I plucked from the ground. I put the container outside with some holes in it bc it's suppose to be cool and moist today

[@LtJAS]

Nice! Thank-you! In what environment/temperature do you store the bag, please?

[@BoomerShroomer]

I store them in my office. It is about 70-75F in there most of the time.

[@tomatobrush3283]

Very advanced technique

[@BoomerShroomer]

Thank you!

[@kristenmamulski3808]

Are the lids you made reusable? How do you care for them in between uses? Do you need to clean them or will the sterilization happening when they are used be good enough?

[@Zack-ul9js]

Hey thanks for the video! I have a question though. Say I buy a oyster mushroom liquid culture online. Can I inject that into my own liquid culture mix and have it duplicate? Thank you!

[@pacificcoasthiking]

Is that a 5” blunt tip needle? How does that needle work in the rubber injection port? If I am getting contaminated LC jars is it more likely from the fae tape or the gasket material port when I am inoculating and getting the liquid all over the inside port area? Thank you for your work and experience

[@deiondyson512]

You are the shit. Thank you

[@joshuatucker7939]

I was wondering can you run this unit without the optional stand? Thank you for your time

[@barbarossalynn7271]

Is it the brown rice that you inoculated? Thank you for beautiful video again.

[@mr.m.o.g.o.m.]

Have you used peptone When you make your liquid culture it’s introduced 1 g a very beginning it has amino acids in proteins that will make a forest of mycelium in liquid culture it’s pretty amazing. Thanks for your previous help it worked out really good for me thank you.

[@BoomerShroomer]

No, I haven’t. Thank you!

[@richardembry9792]

Do you tighten lids down or tighten down and back off a quarter turn I’ve been having a heck of a time getting no contam in my LC’s

[@lalgerielibre9519]

❤❤❤❤❤

[@queerjay1335]

Are they placed in 33-36 f or fridge temp? Are they stored at room temp alongside monotubs? Does putting it in the fridge make it go dormant like it would a grain cake?

[@ClownWhisper]

Here's a question what gauge and length are those needles where do you get those I've never seen those super long needles I've only ever used regular ones or long blunt ones but if you're going through a self healing port it's best to have something sharp or you're not getting through it

[@martinhill8331]

If you're using hydrophobic GE filters you don't need to use foil for your lids. Leaves you plenty for spore prints! Mush love Boomer Shroomer!

[@BoomerShroomer]

Cool thanks!

[@Springfield-eo8jl]

What is hydrophobic GE filters? Is that the filter she puts onto the white lid?

[@poppyjones1892]

Great Tips 😊 coffee filter the LC

[@sugaslate14]

I’m brand new to this. Built a still air box, I have a colonized spawn bag coming. I’ll be making my own substrate. I have everything for agar plates, slants, and am wondering would it be asinine to also have LC? I’m growing lions mane as my first go.

[@Filmafamily93]

Hello. Excuse me can you make champignon mushroom seeds ?? i want to learn to make it

[@almcm8120]

Thanks for your Time Ashley. I am using Raw Honey from a local Bee Farmer, to do LC ..Great Video, and Thanks again for your Help with the Hepa Filter Math!..BtW Did I Mention Theres a Fungus umung us?! Lol! Have a Great Weekend Ashley ! PEACE!

[@BoomerShroomer]

Thank you!

[@jasonpelton7252]

R u using 4% honey and distilled water for ur median?

[@almcm8120]

@@jasonpelton7252 good luck it worked very well 4 me!

[@mrbojangles155]

Could you use capri sun drink pouches and put the live culture directly Into it and paper tape it back ?

[@markking7290]

Do you use the silicone gaskets that come with the plastic lids? If you use traditional metal lids, do you use them and screw on the lid cap????

[@justicemccann7916]

I have a question! If your pressure cooker only reaches 12 psi will that be good enough for LC? I've heard it's good enough for brf jars

[@kenrain404]

I love your videos

[@helpingwonthelp4575]

Are these golden teachers?

[@kumarsanjaysingh]

Thanks for your guidance can we use liquid culture on substrate to grow paddy straw ( Volveriella .Volvecea) mushrooms ?

[@BoomerShroomer]

The liquid culture might take the the paddy straw but I am not 100% sure.

[@sandeepsparks]

Hi. Should I change the syringe filter in lid each time for a new culture. Or how long can I reuse it ?

[@austinmoreno8486]

First video I’ve seen that shows the initial growth, I looked for hours finding a video that didn’t show a week later when it was all cloudy and chunky and I thought my white clumps was mold

[@BoomerShroomer]

Nice! It is hard to see contamination in LC. It is always best to test on agar.

[@faksmakinesi]

Is it mandatory to get the chunk while we spawning in the grain or Is just the liquid enough to spawn a grain bag?

[@Kragoblin]

When making the LC solution, can you prep and sterilize the solution a while prior to adding your culture or does the solution only last so long after sterilization? If I wanted to mix a few of these up and set aside for the future cultures, is that possible, or does it have to be made on the spot? I hope I worded that correctly lol

[@bassboosthq2673]

Literally everyone is using a pre mix I just wanna learn man 🤮🤮🤮on that

[@mkearney98]

“a lot harder said than done” cracked me up 😂

[@naturesADHD]

hey!!! hope your good ! cons and pros vs honey tek??