How to make your own haploid monokaryons - Grab and Drag close-up.

[@BackToSpores]

In less than a year I have gone from never cultivated before, to having 5 monokaryons and currently 10 mon-mon pairings in their F1, with lots of growth and success and failure along the way. Nearly all success has been predominantly thanks to you Ed Grand, and all my breeding has been facilitated by the grab and drag specifically! Thank you for changing my life Ed!

[@edwardgrand]

That makes me very happy! You are the kind of person I make videos for. Mycology changed my life and I think for anyone with a little extra time, money, and interest, it can change theirs too! Thank you for the message. You have given me a much better outlook and motivation for the pile of work I've got to do in a bit. :)

[@PPP-on3vl]

​@@edwardgrandThank u ❤

[@juliansullivan102]

Good morning Dr Ed and thank you for all the excellent content you are creating. Another place to find tweezers is your local cook shop. I found some 7 inch ones that are used for delicate food prep.

[@edwardgrand]

Hell yeah. They got all kinds of cool stuff at the chef shop. Water baths, sonicators, fancy thermometers, forceps, weird chemicals, agar, gellan gum, baker's yeast, nitrous... ;) Good call!

[@dezmushroom6953]

Hey man you have given me the courage to start my Breeding journey ❤🚀 thanks for everything

[@edwardgrand]

Thank you. It is way more straightforward than it seems. Getting those monos becomes a little addictive and the plates multiply like tribbles :)

[@anawesomeperspective3212]

So I'm gonna do my own digging but figured it'd throw the question up here anyways. Once you have monos isolated, that's basically one half of the equation for a fruiting body? And then I suppose there's multiple ways to introduce two monos together...or a mono-dikarion (donation) process? What are the most effective/simplest ways to accomplish this?

[@edwardgrand]

I just throw them both in the same bag of grain.@@anawesomeperspective3212

[@anawesomeperspective3212]

@@edwardgrand easy enough. Thanks!

[@PPP-on3vl]

​@@edwardgrandI don't need a microscope?

[@RiaPsiloBina]

P.s. I love that we both are from Knoxvile and cuss like a sailor!

[@lenskimono2954]

Thanks for all you do Ed, I`m new to this and because of you this hobby has been smooth sailing so far.

[@SPORE_SPORTS]

So glad to see you back making no nonsense informative videos! They really help those of us newer to the hobby 🤙

[@MacCelium]

Thanks man...good stuff as always. Great timing on this vid. Today I'm doing my 1st grab and drag from the last swab I have from my 1st successful grow from years ago. It's kinda sentimental as I went through dozens of contaminated plates for months until I got a tiny clean colony, pulled it off, got for my 1st fruits and had my 1st cup of spicy tea. I was reluctant to use this last swab (sappy, I know) but this technique gave me confidence in getting some spores and saving a bit of clean swab if things don't work out. btw, since I had never ripped a swab apart, the OCD geek in me did a practice run with a clean swab dipped in paprika and a pad of butter. :) In appreciation.

[@jtheatom]

Just poured my own agar plates and watching your vids for guidance. Thanks for the video.

[@ronniereeljr4339]

Just poured more plates yesterday perfect timing. Thank you Mr Grand. I tried the scalpel drag last week.and the ghetto swab thing on a few plates.

[@texaspowerman]

Very nice coverage, if you could do another with prepping the culture to go under the scope to check the monos. I know it’s hard to setup but we could use a how to grab, prepare and plate so we have the correct process down. I end up looking at blobs, trying to dilute them and prep to inspect under the scope. It’s been 30 years since I did prep work, so another video on the new steps would be great. Thanks for the explanation and detailed show and tell. ✌️

[@edwardgrand]

I made videos on 'squash mount' and using the scope. Most of the things you need are there, just have to look for them. Sorry, I don't have time to put them in any order. I just do this for fun. kzbin.info/www/bejne/ZmW9pGSbpsSSq7csi=tkdL23IGxXBLAbXf kzbin.info/www/bejne/p5Wbg2mQdrSLpJIsi=1ufby3-8vyLxWpbb

[@baronsamedi7304]

Thanks Ed

[@phillypharmer]

The timing of these videos has been perfect lol

[@yeahbuddy57]

Thank you for sharing this knowledge. You’re awesome!

[@depraved420]

Tried my first ghetto swab for shits and giggles, kinda want to wait till I get a microscope but I just did it anyway. Had a ksss tub and a TedxTam tub dropping spores at the same time so I swabbed them both and sent it. Got two colonies that were really light and whispy and one that looks normal. So I subbed each colony and made new plates for them and I'm gonna let the grab and drag plate grow out and I'm gonna just spawn them all separate and see what happens lol. Thanks for the education Sir! Got a bunch of financial problems so I gotta wait on a scope for a while, you and DK definitely inspire me into wanting to breed. Thanks as always ED. 🍄❤️

[@Houseofmycology]

Great video Edward

[@minuteman603]

Bringin monos to the common folk, love it 😂😂 good work man I've been reading a lot about this and its great to see a video that puts it into perspective!! Getting a scope very soon to get deeper into the game 😅

[@RiaPsiloBina]

Ill come be your videographer any day! Just feed me authentic Pad Krapow Gai, teach me skills to be lab assistant, and show me some of Thailand ! Ill be trying the Grab and Drag in the next few days when I get back to Nashville. Thanks so mush! 🍄❤️

[@jeremytalbot8565]

Thank you Edward!!

[@PsychonautMarine]

Love the demonstration! 🖤

[@donniebrasco11]

❤❤

[@MycoAlan]

Great video as always Dr.Ed, do you have a video on making slides?

[@edwardgrand]

yes. Something about 'squash mount'.

[@BlackLaryBird]

Dr Ed . The they them took Yoshi down ? I don’t understand the rhyme or reason to this . I guess this is how you know you are doing well now a days . I love your videos thank you for them .

[@WarrenHill-tl9tf]

Great video man.

[@Mahalorider]

Aside from marking up plates, do you have a catalog/journaling system for all this?

[@edwardgrand]

No. I would spend more time writing records than I would doing myco stuff.

[@boi.7377]

Most induction heaters only heat up ferrous material (aka iron)- I believe syringes are non-ferrous

[@edwardgrand]

I thought so too, but I had a few leftover from another brand that heated up in the coil. The newer ones I have don't do anything though.

[@funghi-farm]

Thanks for this video. Can you do one how you check them on the scope 🧬?

[@edwardgrand]

Doing videos on my scope is excruciatingly difficult.

[@funghi-farm]

How you prepare your slide ? Thanks for this video. That helps really! Greets from Spain

[@edwardgrand]

kzbin.info/www/bejne/ZmW9pGSbpsSSq7csi=22K_jlLo--hg9hUJ@@funghi-farm

[@edwardgrand]

kzbin.info/www/bejne/harXZ4FvjtN0q7Msi=job0G0iEZCBpC7Ki@@funghi-farm

[@rudoduck]

I am extremely grateful for your responses. Would it be possible to get a promo code please? I am finalizing my order. Thank you so much

[@edwardgrand]

I don't do promo codes.

[@rudoduck]

ok

[@Houseofmycology]

How many hours a day should I keep my light on during fruiting?

[@edwardgrand]

I keep my LEDs on 24/7, all the time.

[@Houseofmycology]

@@edwardgrand I bought a light for mushrooms from Midwest grow! I keep it on a 12/12 cycle! Should I do 24

[@edwardgrand]

@Houseofmycology should be fine. The light cycle isn't important.

[@Houseofmycology]

@@edwardgrand ok cool thank you

[@Houseofmycology]

What’s the difference between a mono and a dia? What’s bg stand for on the plate?

[@edwardgrand]

Mono is haploid. One nucleus per cell. Dikaryon is two haploid nuclei in one cell. BG#2 is just an isolate name.

[@Houseofmycology]

@@edwardgrand I really need a microscope

[@edwardgrand]

Amscope B120C@@Houseofmycology

[@Houseofmycology]

@@edwardgrand thanks man I’ll look it up right now

[@Houseofmycology]

Which one do I want a mono or dia? Mono right? But why?

[@TeamFortressTwoGaming]

how much magnifaction do you need/recommend to check for clamps

[@edwardgrand]

400X. 1000X if you are new to it. But my scope uses oil at 1000X. So messy, so I stick to 400X.

[@MrHavNaught]

🤙😘

[@Fairy.Tail.Scalez]

Hey Ed how are you today? Do you have an email I can contact you on or some way we can communicate? I would be interested in a mentor session and have some ideas I want to try. I was hoping you could explain some of the science behind them and the chances it would or wouldn't work.

[@edwardgrand]

You can find me on FB and IG. I'd rather not put my personal email here.

[@HoboTech-xl3wg]

ordered a microscope after watching this.

[@Houseofmycology]

Do you have any truffle producing genetics on your site? I didn’t know how to spell the real word for the truffles😂

[@edwardgrand]

ATL #7 research syringe

[@Houseofmycology]

@@edwardgrand ahh ok what atl stand for?

[@edwardgrand]

Atlanta. It is a P. tampanensis isolate.@@Houseofmycology

[@Houseofmycology]

@@edwardgrand are truffles good? I heard it takes double what the mushies take and harder on the stomach? Is that true?

[@edwardgrand]

They are less potent by mass. But they are generally consumed fresh. I think they are easier on the stomach. You must be busy watching videos. LOL Glad to get the comments.@@Houseofmycology

[@rudoduck]

hello, I live in Canada. Do you deliver spores to my country? merci.

[@edwardgrand]

Yes. No problem. Very discreet packaging. Might ta 3-4 weeks for free shipping option, but it flies under the radar better.

[@rudoduck]

Living in a Nordic country, can freezing spores during delivery harm their viability?

[@edwardgrand]

I'm not too sure. Prints or swabs should be ok, but MSS might be problematic.@@rudoduck

[@rudoduck]

@rudoduck @rudoduck il y a 2 heures you have a lot of hybrid strains that I don't know, can you name me your 5 most powerful varieties, I love the visual effect ,thanks for your answers

[@edwardgrand]

They are all of comparable strength if grown correctly. @@rudoduck

[@Houseofmycology]

Do you ever grow in manure?

[@edwardgrand]

I added some manure to my recent TTBVI Pan grow. about 10% by volume with chopped straw.

[@Houseofmycology]

@@edwardgrand ttbvi what’s that stand for? I know it’s an isolate!

[@edwardgrand]

Tamarind Tree British Virgin Islands@@Houseofmycology

[@Houseofmycology]

@@edwardgrand ahhhh ok cool thanks man

[@Houseofmycology]

@@edwardgrand when growing in bags do you fan and mist ? What about shoeboxes do you fam or mist during the first flush? I’m growing in bags right now should I just leave the corner open or cut a slit below the filter patch? I have the bags in a clear tub.