Making Mushroom Grain Spawn in the Lab with Agar Mycelium | Southwest Mushrooms

Mushroom Mike shows his technique for inoculating sterile grain with agar mycelium to create grain spawn in the lab.
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▶ Cultivating fresh gourmet mushrooms at our urban grow farm. Mushrooms with amazing flavor and full of beneficial nutrients. Our passion is to offer every customer the freshest product in the market. Owner "Mushroom Mike" enjoys sharing the science of mycology while serving top chefs, everyday cooks, and those who enjoy a healthy lifestyle with premium mushrooms.
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[@seancollins-cruz2134]

New Southwest video? Never clicked anything faster! 🙌🏻

[@LadyElaineLovegood]

Thanks for not editing out the fumble. You gave us useful information plus it's comforting to see that even professionals have those moments.

[@huntakilla1234]

Hot damn! I thought you guys went out of business or something happened, seeing how uploads came to a grinding halt. Glad to see you're going strong! 👍

[@Catanna1953]

So glad you're back! Because of your earlier videos I learned how to successfully grown mushrooms in my basement!!! Thank you!!

[@Crazyking-mf8qi]

Driving across the country and sitting in my hotel room scrolling through instagram then I see a post. After waiting the duration of a human pregnancy, life is good, a new Southwest Mushroom video has blessed us with its presence

[@shroomieshrooms6551]

Even though I know this and have done this about 100 times I still feel like I'm learning something watching your videos

[@eezybreezy414]

hey Mike, just purchased like eight bricks from you brotha,about to start this mycology journey....Wish me luck!

[@AliceDee025]

You’re an inspiration and well deserving of the noble peace prize for being the most peaceful person I’ve ever seen. I’ve dropped my plate stack in lab before and my reaction was much louder. Thanks for not editing that out, and thank you for these videos, they’ve been priceless.

[@vancegilmore245]

Mike would make a great Chewbacca for halloween. 😁

[@emilyji868]

glad to have you back!

[@andrewleatherman4931]

You really do care about mushrooms. I love your ever evolving lab setup and your continued standard to maintain a strict clean lab space. Love your enthusiasm and I hope Southwest Mushrooms continues to prosper.

[@azoutlaw7]

Yay! Nice to have a new video from you! Thank you!

[@plantagardenforever]

I can't wait to move and start growing my own mushrooms! Welcome back!

[@Vergazillion]

Was so surprised to see a new video from this channel and I am thankful. I love watching these and hope you guys keep uploading. Thanks again

[@VinegarAndSaltedFries]

So happy to see a video from you really brightened my day. Keep up the great work.

[@thehipbeekatherine9520]

I could watch this non stop. Years ago I trained as a medical lab tech and my favourite rotation was microbiology - this makes me happy - I need to do this again ❤️

[@theanalymous]

Glad to see you back 🤩

[@tonystewart7624]

Happy to see you putting out new videos brother!

[@Snufulufugus]

Welcome back! Love your guys content!

[@miguelrobles4907]

I'm so glad you are making videos again!!!

[@dirtdiggerjerry]

Just getting started. Thanks for your help.

[@widnmbodken]

heyyy you are back! glad you are okay.

[@paulareeveslmt]

Love your videos, glad you're back! Woo hoo! Just grew our first Lion's Mane and Chestnuts are fruiting now. Thanks for your excellent videos/info.!

[@Officialunpopularopinion]

Sooo happpy to see videos are up and going!!🙏🏽❤️❤️❤️❤️

[@fraq]

Best content on youtube! Love it. Relaxing and informative.

[@7115themike]

Love what you do man. I have watched every last one of your videos. And you personally inspired me to start growing flushes. I have done well. And will be hitting the farmers markets in less than 2 months now

[@markmac9515]

Respect for including the dropping of the petri dishes. I have really enjoyed your easy way that you show us how you work.

[@KaleidoKurt]

Video production is getting way better. Thanks for the information!

[@realfunguy4158]

So great to see more content from you guys, always super inspiring and informative. Awesome!

[@CriticoolHit]

Glad you're back. Was worried there for a while.

[@SPLITKOOKS]

Yeahhhhhh brah, educate the myco curious! Thanks for all your efforts, everyone gain by this knowledge being out there!

[@cornevermaak6334]

Good to see you back bro!

[@benji7972]

Absolutely loved that funky intro 🤌🏼

[@housearea]

Finally a new video!!! Long long waited!

[@SethxTricky]

Welcome back!

[@Davewheelz]

👀 spaceztrip

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@philstarantulatime4742]

Really good video! I really like how professional you do this job 👏

[@zuhawk15]

Got to make some purchases!

[@zaidaslaughter700]

i missed you guys so much!!!!!!

[@sekinafi29]

Love to see y’all again ❤️

[@mutulicaaa]

Thank you for being an inspiration.

[@Fog876]

Good seeing you again man. You remind me of my future self with a beard lol! Thank you for all the free knowledge.

[@threeiem]

YAY YAY YAY!!! Missed you guys!

[@miyagisan9971]

Welcome Back Master !

[@Davewheelz]

👀 spaceztrip

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@bonsaifungi7150]

New video! welcome back!

[@kakrot3]

It's about time!!

[@chudwickmt]

You rule. I’m hitting you up when I come back to AZ. I lived in E. Mesa for a few years

[@MooreDick]

Love this educational content :) it shows why mushrooms are worth buying instead of trying to grow

[@KeizerSinbad]

The intro was pretty darn good. You e stepped up your filming game.

[@zorg621]

New video, thanks Mike! More vids please!

[@zorg621]

That was great seeing your petris fall. Thank you for leaving in the errors. When watching you it's easy to think "wow mike has it all figured out" and then be discouraged by the mistakes we make. When we see you make mistakes it's a good reminder.

[@midnightkola]

OH MY GOSH THEY ARE BACK!!!! I just checked back on this channel to see if anything was posted and there are new vids!!!! Love you guys!

[@trip_lord1atinstagram890]

👆👆👆👆I recently started microdosing and using psilocybin coupled with therapy. It has helped opened my emotions up

[@trip_lord1atinstagram890]

Look him up 👆

[@johnkoury1116]

Really awesome videos my friend. You are an inspiration !!!!!

[@plantagardenforever]

Nice flow set up and nice autoclave!

[@megs4163]

Hot damn I’m obsessed. I’ve been working in the lab with agarose for gel electrophoresis but all I think about is doing this kind of stuff. Thanks for sharing!

[@donmandotron]

Thanks so much for making these videos. Can you do one on how you keep strains going?

[@censoredbyyoutube2639]

Beautiful work as always

[@Daniellobato69]

Your a beast bro! I really learn a lot from your videos. Thank you.

[@luketakahashi2003]

YOU'RE BACK! Heck yeah

[@Davewheelz]

👀 spaceztrip

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@R-Rpt]

Thank you Mike

[@connorholgate1715]

Hell yeah new video!

[@pablorojaz2343]

Great job 👏 I love your videos Saludos desde Mexico 🇲🇽

[@anonymousjustice4357]

BROTHER ONLY WISHING YOU ETERNAL PROSPERITY TO YOU AND YOUR BUSINESS ! 💪🍄💪🍄💪🍄💪👏

[@willmfg]

Love the content man. Truly grateful to have you as a resource of mycology information.

[@smartjared7203]

I get my from an online store his got shroom, psychedelic and microdosing products well refined also ships to anywhere discreetly, can also guide you on anything....?

[@smartjared7203]

His on Instagrams, with also telegrams with,,

[@smartjared7203]

*Mycopete%

[@e30techgaming22]

Glad to see another video. Mike you inspired me to start my own mushroom cultivating business

[@chicofromph33nix64]

I would love to do the same... just don't know where to start... wishing you all the success with your business... MUSH LOVE AND RESPECT

[@joshdobesh]

@chicofromph33nix64 start small. Still air box. Get a liquid culture from the internet. Get Paul Stamets book on how to grow. Research Research Research! What just keep learning

[@chicofromph33nix64]

@@joshdobesh I got a flow hood on the way and getting a still air box in the near future.... I have a few syringes of spores and LC already... I've inoculated 4 bags but 2 just stalled out on me.. the others look like they're coming along.. i got some books I'm reading from Terrance McKenna and learning from videos.... I'm definitely going to look into that book you recommend, thank you very much... I've been interested in growing mushrooms for about 6 months now,but I freaking love it already... plus the community is so loving and everyone enjoys helping others out... ever since I've watched southwest mushrooms videos I felt this is the road I wanna take... their warehouse is just a mile up the road from me.. I want to get on that level someday.. I need to take a trip over there to see their set up... i guess start with friends and family and grow from there... you're right,just gotta start small and take baby steps... I'm looking way ahead of myself... I'll make something of this, I know it... sorry for the novel,but REALLY APPRECIATE THE ENCOURAGEMENT...💯💪🏽🍄❤

[@tadeumagalhaes5468]

The videos are better and better... Thanks so much for so much info! Keep going guys! Love ya!

[@Davewheelz]

👀 spaceztrip

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@bryanjames5256]

Amazing content as always. So helpful to us newbies. What is that cylindrical thing I'm assuming your sterilizing your lab instruments in?

[@fewdz]

Hell yeah.

[@Davewheelz]

👀 spaceztrip

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@captaintoyota3171]

This guy like dedicated his life to this process and its awsome. Ive tried its not ez to perfect fungus growing

[@gastoniragui9389]

genial que dejaste tu error con las placas, hasta los mejores se equivocan, gracias por tus aportes bro increíble tu laboratorio abrazo grande desde Argentina!

[@wpchristie1]

I love the spicy new intro

[@johnbrzenksforearm8295]

Do you ship fresh mushrooms from online?

[@EggsForDayz]

Love the intro

[@ginz9r]

Welcome Back

[@Davewheelz]

Check spaceztrip

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@Eddy-nn7wj]

Hey @Southwest Mushrooms! Amazing videos, inspired me to spread the spores too! How long do you soak the wheat before sterilization? Do you preboil it?

[@markgarry7866]

I really enjoy your videos, thank you...I'm just starting out, would you suggest mixing different agar cultures of same species from different suppliers, or keeping each separate? Was just wanting to take best growth from each dish and mixing into my grainbags. What are your thoughts? Will be oysters and lions mane to start.

[@EggsForDayz]

Sound music for the intro and outro are great do this

[@priyanshuvmore1907]

Finally....

[@lowcountryfungi]

Appreciate you keeping it real with fumbling the plates ha! What do you use to incubate plates?

[@wowza3611]

Do you have any videos preparing your grain and making the bags?

[@BaltimoresBerzerker]

Anyone have experience with tryptone v peptone for making agar and LC? Can anyone recommend one over the other or is a mixture ideal? Thank you so much for any guidance.

[@Johnny-ur2st]

Finally I was a bit worried

[@Davewheelz]

👀 spaceztrip

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@charlesficherwcoffeebreaks7521]

#enjoyingwatermelonog thanks for the quality content #peace

[@chiliewhilie84]

I have a Lc of reishi that just passed qc on agar, but as I was agitating it, I can see a hair of mine? Is that going to be a problem?

[@eddie2551]

I have a question what is the difference between a 5lb block & a 10lb block do you get more yield from the 10lb block?

[@firemedic1648]

Yesss!

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@monkey7707]

i wish you lived in my area dude

[@stevensanborn4861]

what was that name of the music you had at the end ? got intense suddenly :) and i dig it

[@eastindiaV]

I've been making soma, which is just wild mushrooms, fermented with yeast, sugar, leaves, and distilled water. Looks like it's beer, but seems to be a semi active liquid culture in terms of growing them. The spores settle to the bottom, just like yeast. I don't have a sterile lab, I'd be curious if you could prove this would work as a viable spore syringe, without being steam sterilized, or isolated. I'd also be curious if that would work as is for sterile spawn, or pasteurized bulk sub. It would further explain how ancient people worked with mushrooms without sterile techniques. From Soma medicine, I think they would inoculate piles of logs, and once they would be colonized, they'd be moved into caves. I think they used to put the spawn in clay casks inside the cave, if I'm not mistaken. This would have been around and before the dark ages. I've also been studying Ectomycchorizae, and I think Soma can do similar things to mushrooms, as in integrate foreign DNA into their genetic code... seems like a labs job to prove tho lol

[@tonystewart7624]

That's interesting, I love reading about different processes and especially older ones.

[@BaltimoresBerzerker]

I'm a total history nerd so if you have any good reading material on what you're discussing I would very much appreciate it.

[@texasermd1]

Very cool place you have there. What is the reasoning behind liquid vs agar for inoculation? Thanks for the video. 👍🏼👍🏼

[@Fog876]

Liquid tends to be better and colonize quicker.

[@Johnny-ur2st]

Mike what kind of pen did you use on Petrie dishes. If I use permanent marker the alcohol washes it away instantly. Let me know my friend

[@C0okiesforfun]

What is that device called that you're using for heating up the blade?

[@KarlMiller]

Why is contamination bad? Is it an issue with yield or a health risk considering some people eat mushrooms grown on manure in the wild?

[@ianhowie3628]

What pressure cookers are they?

[@blairlaird]

Why use the wedges rather than inoculation with a syringe? I wasn't clear on the benefits of doing it that way

[@birdzy]

Is that the 27.3 Quart benchtop autoclave from All American?

[@HassanKhan-jx7nx]

I love each and everyone of the videos. Quick question though, what's that spray solution you're using on everything to sterilize it? Is it a mix of water and alcohol or ..?

[@TheLeah240]

It’s isopropyl alcohol

[@amnezjator]

Can You guys correct me, did You just sterilized OPENED filtered bags? am i right?

[@harshaa7079]

Welcome back

[@Davewheelz]

👀 spaceztrip

[@Davewheelz]

They are on Instagram

[@Davewheelz]

They got good psychedelic products such as mushroom spore lsd dmt chocolate bars and lots more they ship discretely worldwide

[@skiddysm]

Do you dry bag your spawn?

[@ivanangelototola2472]

Sou do Brasil. Gostei das dicas. A minha pergunta é: Quando uso grãos de geração 1 para ágar ou cultura líquida, tanto o ágar quanto a cultura líquida são considerados que geração? Obrigado.