How to pack a Tricorn chromatography column

Learn how to pack a Tricorn chromatography column. This video gives guidance and information for successful column packing.
Learn more on our website: www.cytivalifesciences.com/so...

[@jonathanridder]

0:07 Pair a meters?

[@guadalupeluna4095]

Hi I´m Guadalupe Luna. I just purchased the Superdex 75 10/300 column. I'm trying to run the column performance control. According to the instructions in the manual, I must maintain a flow rate of 0.75 ml / min at room temperature and inject 100 microliters of 0.5% acetone, however, the flow that comes out of the column is only 0.1 ml / min. min and not 0.75 ml / min. What could be the problem? I hope you can help me. Thanks.

[@Cytiva]

Hi Guadalupe, thank you for reaching out to us.I'm sorry to hear about the issue you are having. I would recommend that you reach out to our scientific support team here www.cytivalifesciences.com/support/scientific-support Please let me know if you have any problems or need any extra support. Best wishes Sophie

[@Cytiva]

Hi Guadalupe, Apologies that you are still waiting for someone to contact you back about this. I will reach out to scientific support to see if I can chase this up for you. Best wishes, Sophie

[@guadalupeluna4095]

@@Cytiva Thank you very much Miss Sophie. I appreciate her kindness. I hope she can help me. Regards!