How to separate DAB staining in a IHC with color Deconvolution using ImageJ

#DAB #IHC #imagej #color #deconvolution #science

[@subhratasarma2781]

How to install colour deconvolutation ? Its not present in the image j applications

[@bioinfotips]

Do you have images Fiji? I'm pretty sure I did not had to install anything in mine.

[@subhratasarma2781]

@@bioinfotips ok. Actually I had image j. Would you kindly explain how to calculate the staining intensity not the percentage area.

[@bioinfotips]

Sure. Basically, you do the same thing but on the "measurements" menu, go to "set measurements" and make sure the "intesnsity" ones are checked