How to set Scale Bar using ImageJ software | Microscope | Imaging

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I am a 2nd Year PhD student working in the field of Muscle Biology. Visit www.akashmitra.in to know more about me.
If you would like to talk about the video or anything in general, just drop a word on Instagram or if you have a really long question then shoot me an email at [e-mail]. I would surely respond 😃

[@somishrivastava5477]

Thank you. It will be really helpful.

[@ITGIRL922]

after hours trying to find about imagej, finally. thank you for this video and the scale bar reference, sir. your audio is so clear, i can immediately understand what you say.

[@akashmitrabiology]

Really appreciate that. Glad I could help

[@shrinathmb]

Hello sir , how to rorate the scale bar , which is horizontally place ?

[@easyhack5859]

What about those pictures which we take through camera so how can we know their magnification level ? Please guide

[@thesunshine3804]

Thank you so much sir, your video really help me out🌼

[@akashmitrabiology]

You are most welcome

[@bugisrantau77]

Big thanks brother, from Indonesia😊

[@akashmitrabiology]

Glad I could help

[@hediyeh_savari]

Thank you!🤩

[@akashmitrabiology]

You’re welcome 😊

[@easyhack5859]

How we can know the level of magnification of pictures especially those pictures which are taken through high speed cameras ? Please someone guide me

[@ziqilim558]

May I have the imagej reference?

[@deadpool4859]

Sir i have mailed you regarding the measurement image kindly send me the image. As the link provided isnt working .

[@ScintillatingmomentswithViv]

Thank you Mr Akash, I am new here and have a question Does the resolution of my photomicrograph affect the magnification I would choose on the reference image

[@akashmitrabiology]

I don't think the resolution will be a factor because the magnification won't be affected

[@user-jr9tx9sy1m]

Thank you for your kind assistance, sensei. However, I tried making use of the reference scale bar image but it is doubling the actual length of each line I measure.

[@akashmitrabiology]

Thats not possible actually

[@IranianVibess]

Hello Can we use this reference image (all scales) for all of microscopic images including confocal microscope images?. Thank you

[@akashmitrabiology]

Yes. You can use this for any microscopic images including confocal

[@abugomana2648]

how to draw a square out of a specific area of a picture i want to magnify? thnk you

[@akashmitrabiology]

Use the square tool and crop it using cmd+shift+X

[@premasingaravel6014]

sir, can you say how to draw line for 60x image

[@akashmitrabiology]

Right now I don't have a scale reference for 60x.

[@Regimented]

Does one have to save a scale on every image. Even when you have many pictures

[@akashmitrabiology]

Not necessary. You can show in one image on the panel and the rest you let it be

[@serayzoratarhan2590]

Thank you

[@akashmitrabiology]

You're welcome

[@ayushmegotia501]

Sir,in my thesis i have to measure retinal vessels diameter,,,sir how i get scale bar for it

[@akashmitrabiology]

In what magnification were the images taken?

[@purushottampawar375]

I want to add a scale bar on microscopic images with 200X magnification. These images are scanned from the lab report, how can I add a scale bar to them

[@akashmitrabiology]

You need to have a reference scale of 200X and you can follow this video. Unfortunately I don't have a reference scale of 200X

[@Am-yo4hx]

thanks

[@akashmitrabiology]

You're welcome!

[@thatsmeJR]

Pls Use Hinglish or Put English Subtitles Your Channel is far ahead of time . Current Gen is not ready for this amazing content. Thank You 💞🤗 for providing it.

[@akashmitrabiology]

Fundamentally, my hindi is rusty and I use English so that everyone around the globe can use the videos. I will try and put subtitles for sure. Thanks for the suggestion.

[@cathrinejensen9993]

Hi, i can't access the reference image unfortunately

[@akashmitrabiology]

@@cathrinejensen9993 Hi Cathrine. The site is acting weird. Apologies for that. But, if you can drop an email at [e-mail], I can send the image to you.

[@paolacarrion4170]

@@akashmitrabiology Hi! Can I email you too for the reference image?

[@akashmitrabiology]

@@paolacarrion4170 Ya sure!

[@AneeqaMunawar-ts6lg]

hi sir! kindly send me the reference image. I am greatly in need of it..

[@manelialavioun552]

Thank you for the video. Can I use the refrence image for inverted microscope images which I took with my mobile camera?

[@akashmitrabiology]

Yes! You can use it.

[@manelialavioun552]

Thank you. The last question, can I crop my images before adding the scale bar? Or it have effects on the measurements

[@akashmitrabiology]

@@manelialavioun552 you can set the scale according to the uncropped image. After that when you crop it it will adjust according to the aspect ratio

[@manelialavioun552]

@@akashmitrabiology I've read somewhere that you can crop the image before setting the scale bar, you mean it is wrong? So I have to redo all again.

[@user-us8tt3xg1w]

Do you have a scale reference for 2X?

[@akashmitrabiology]

Unfortunately I don't

[@justinxu9593]

Thank you. Just wondering, why does this reference image work for any type of microscope?

[@akashmitrabiology]

Because whichever microscope you use, 20x or 10x will be universal magnifications. The magnification of 20X in microscope 1 would be same as that of 20X in microscope 2. I hope this answers your question

[@onimisibethel2802]

​@@akashmitrabiology please send me the reference image

[@khoinguyenlehoang6000]

hello sir, how can i get your reference image ?

[@akashmitrabiology]

Drop me an email at [e-mail]

[@shabbirsain7470]

Dear sir I am not able to understand! Why did u change 75 um to 100 um at 20x magnification. Kindly if u got my msg plz help me to sort out

[@akashmitrabiology]

We are not changing the magnification. We are just changing the length of the scale bar. You can set it to whatever you want once you have the measurements down for any magnification.

[@haileydeckers]

Thank you for the video. When I insert the scale bar from tools it shows in inches instead of microns, how can I adjust this?

[@akashmitrabiology]

Hi. All you need to do is change the units to um

[@haileydeckers]

@@akashmitrabiology I changed in the "set scale" settings, but I don't believe the conversion is correct as it would set scale bars of equal distances for 2 nm and 2um... My image is 1920x1440 pixels.

[@akashmitrabiology]

@@haileydeckers I am unable to understand over this conversation. Could you drop me an email and share your image?

[@srimathiramesh5799]

Hello! Could you please share the link to the reference image. I have already mailed you.

[@akashmitrabiology]

I think I had replied to your email

[@poorviasthana1587]

after dragging the main image, i.e., fluorescent image into the imageJ , no dialogue box as such @7:45 appeared for me

[@akashmitrabiology]

It will appear only if you set the scale bar at global in the previous step

[@poorviasthana1587]

@@akashmitrabiology Ya I had set up the scale bar at the prior step but still it is coming like that. Can you please also provide the method of how to count the number of live and dead cells in a image of Live/Dead assay, using ImageJ and that also of a specific RoI (Region of interest) ?

[@dewipuspita5917]

can i have this image scale bar 1.000x ?

[@akashmitrabiology]

HI. Could you email me at [e-mail]? I will send the image

[@ronalduscaesarianoekaputra6873]

Dear Akash Mitra Sir, I have sent request email for image scale reference. Please kindly need your help. Thank yo Sir

[@flasardela]

Hi Akash, thank you for the helpful video! I sent you an email to get the reference image, an you let me know if you received it please? Thank you!

[@akashmitrabiology]

Hi. Did I reply back to you on your email. If not, I am very sorry. Please send me another email.

[@flasardela]

@@akashmitrabiology Hi! I don't think I received your reply, so I just sent you a new email now. Thank you so much!

[@NMMEDIXITHEGDE]

sir i have sent you mail requesting imagej reference image ,i request you to look in to it.

[@akashmitrabiology]

I have emailed you the link. Sorry for the delay.

[@yousratouami1900]

Hello, can you please send me the reference image he sent you? I would be so grateful

[@mbitsakitha3790]

Hello i find your video very interest and address the challange i am having with scaliing please send me the reference image. Thanks@@akashmitrabiology

[@basmazohair3241]

Could you please send me the reference image. I've already sent you an email. Thank you very much.

[@LarissaMayraSilvaRibeiro]

did you get the image?

[@anishak.c.5442]

Hello. I mailed you for the reference image. You haven't replied me. Please address this. I have my thesis defense next month.

[@akashmitrabiology]

Hi. I have sent the image to you

[@jenicaashleypantollana6104]

Hello! I've sent you an email regarding the reference image, sir. Hope that you could address this. Thank you!

[@akashmitrabiology]

I think I emailed you back. Please let me know if I missed it

[@flasardela]

Hi @jenicaashleypantollana6104, if you received the image, could you also forward it to me please?

[@athulkrishnanr1223]

Hey, Akash... I have also mailed you. Can you pls check

[@akashmitrabiology]

Hi. Did I reply back to you?